Kowsari, Reza, Yazdian-Robati, Rezvan, Razavi, Bibi Marjan, Pourtaji, Atena, Ghorbani, Maryam, Moghadam-Omranipour, Hediye, Hosseinzadeh, Hossein, Lari, Parisa, Abnous, Khalil. (1395). Recognition and characterization of Erythropoietin binding-proteins in the brain of mice. , 19(9), 946-952. doi: 10.22038/ijbms.2016.7594
Reza Kowsari; Rezvan Yazdian-Robati; Bibi Marjan Razavi; Atena Pourtaji; Maryam Ghorbani; Hediye Moghadam-Omranipour; Hossein Hosseinzadeh; Parisa Lari; Khalil Abnous. "Recognition and characterization of Erythropoietin binding-proteins in the brain of mice". , 19, 9, 1395, 946-952. doi: 10.22038/ijbms.2016.7594
Kowsari, Reza, Yazdian-Robati, Rezvan, Razavi, Bibi Marjan, Pourtaji, Atena, Ghorbani, Maryam, Moghadam-Omranipour, Hediye, Hosseinzadeh, Hossein, Lari, Parisa, Abnous, Khalil. (1395). 'Recognition and characterization of Erythropoietin binding-proteins in the brain of mice', , 19(9), pp. 946-952. doi: 10.22038/ijbms.2016.7594
Kowsari, Reza, Yazdian-Robati, Rezvan, Razavi, Bibi Marjan, Pourtaji, Atena, Ghorbani, Maryam, Moghadam-Omranipour, Hediye, Hosseinzadeh, Hossein, Lari, Parisa, Abnous, Khalil. Recognition and characterization of Erythropoietin binding-proteins in the brain of mice. , 1395; 19(9): 946-952. doi: 10.22038/ijbms.2016.7594

Recognition and characterization of Erythropoietin binding-proteins in the brain of mice

Iranian Journal of Basic Medical Sciences
مقاله 5، دوره 19، شماره 9، 2016، صفحه 946-952    اصل مقاله (1.03 M)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2016.7594
نویسندگان
Reza Kowsari1؛ Rezvan Yazdian-Robati1؛ Bibi Marjan Razavi2؛ Atena Pourtaji3؛ Maryam Ghorbani4؛ Hediye Moghadam-Omranipour1؛ Hossein Hosseinzadeh3؛ Parisa Lari3؛ Khalil Abnous5، 6
1Department of Biotechnology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
2Targeted Drug Delivery Research Center, Department of Pharmacodinamy and Toxicology, Mashhad University of Medical Sciences, Mashhad, Iran
3Pharmaceutical Research Center, Department of Pharmacodynamy and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
4Department of Pharmacology and Toxicology, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran
5Pharmaceutical Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
6Department of Medicinal Chemistry, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
چکیده
Objective(s): Erythropoietin (EPO), is a 34KDa glycoprotein hormone, which belongs to type 1 cytokine superfamily. EPO involves in erythrocyte maturation through inhibition of apoptosis in erythroid cells. Besides its main function, protective effects of EPO in heart and brain tissues have been reported. EPO has a critical role in development, growth, and homeostasis of brain. Furthermore EPO has great potential in the recovery of different brain diseases which are still under studying. In this research, EPO binding pattern to brain proteins in animal model was studied.
Materials and Methods:EPO antibody was covalently crosslinked to protein A/G agarose. in order to interact between EPO  and its target in brain,  about 5µg EPO added to brain homogenates(500ul of 1 mg/ml) and incubate at 4ο C for 30 min. brain tissue lysate were added to agarose beads, After isolation of target proteins(EPO - protein) both one and two-dimensional gel electrophoresis were performed. Proteins were identified utilizing MALDI-TOF/TOF and MASCOT software.
Results: This research showed that EPO could physically interact with eightproteins including  Tubulin beta, Actin cytoplasmic 2, T-complex protein 1, TPR and ankyrin repeat-containing protein 1, Centromere-associated protein E, Kinesin-like protein KIF7, Growth arrest-specific protein 2 and  Pleckstrin homology-like domain family B member 2.
Conclusion: Since EPO is a promising therapeutic drug for the treatment of neurological diseases, identified proteins may help us to have a better understanding about the mechanism of protective effects of EPO in the brain. Our data needs to be validated by complementary bioassays.
کلیدواژه‌ها
Brain؛ Erythropoietin؛ Immunoprecipitation؛ Proteomic screening؛ Target deconvolution؛ Neuroprotective effect
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