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Li, Wei, Zhao, Xue, Yu, Ting-Ting, Hao, Wei, Wang, Guo-Guang. (1400). Knockout of PKC θ gene attenuates oleic acid-induced acute lung injury via reduction of inflammation and oxidative stress. , 24(7), 986-991. doi: 10.22038/ijbms.2021.56908.12695
Wei Li; Xue Zhao; Ting-Ting Yu; Wei Hao; Guo-Guang Wang. "Knockout of PKC θ gene attenuates oleic acid-induced acute lung injury via reduction of inflammation and oxidative stress". , 24, 7, 1400, 986-991. doi: 10.22038/ijbms.2021.56908.12695
Li, Wei, Zhao, Xue, Yu, Ting-Ting, Hao, Wei, Wang, Guo-Guang. (1400). 'Knockout of PKC θ gene attenuates oleic acid-induced acute lung injury via reduction of inflammation and oxidative stress', , 24(7), pp. 986-991. doi: 10.22038/ijbms.2021.56908.12695
Li, Wei, Zhao, Xue, Yu, Ting-Ting, Hao, Wei, Wang, Guo-Guang. Knockout of PKC θ gene attenuates oleic acid-induced acute lung injury via reduction of inflammation and oxidative stress. , 1400; 24(7): 986-991. doi: 10.22038/ijbms.2021.56908.12695

Knockout of PKC θ gene attenuates oleic acid-induced acute lung injury via reduction of inflammation and oxidative stress

Iranian Journal of Basic Medical Sciences
مقاله 16، دوره 24، شماره 7، مهر 2021، صفحه 986-991    اصل مقاله (709.52 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2021.56908.12695
نویسندگان
Wei Li1؛ Xue Zhao1؛ Ting-Ting Yu2؛ Wei Hao2؛ Guo-Guang Wang* 1
1Department of Pathophysiology, Wannan Medical College, Wuhu, China
2Experimental Center for Function Subjects, Wannan Medical College, Wuhu, China
چکیده
Objective(s): Acute respiratory distress syndrome resulting from acute lung injury has become a momentous clinical concern because of high morbidity and mortality in discharged patients with pulmonary and nonpulmonary diseases. This study aimed to explore the effect of protein kinase C (PKC) θ gene knockout on acute lung injury.
Materials and Methods: Wt and PKC θ gene knockout mice were intravenously injected with oleic acid to induce acute lung injury. Pulmonary capillary permeability was assessed via measuring lung wet/dry weight ratio and level of protein in bronchoalveolar lavage fluid (BALF). Histological changes were used to examine acute lung injury. Malondialdehyde (MDA) level, superoxide dismutase (SOD) activity in serum, together with inflammatory cytokines including interleukin (IL)-6 and tumor necrosis factor-alpha (TNF-α), were determined. Furthermore, the expressions of heme oxygenase (HO)-1, nuclear factor kappa B (NF κB), and inhibitor of NF-κB alpha (IκB α) were detected in the lungs.
Results: PKC θ gene knockout decreased lung wet/dry weight ratio, reduced levels of MDA, IL-6, and TNF-α in serum together with level of protein in BALF. Furthermore, PKC θ gene knockout increased the activities of SOD.  Knockout of PKC θ was also observed to increase expression of HO-1 and reduce levels of p-NF κB and p-IKB α in the lungs.
Conclusion: These results suggest that PKC θ gene knockout attenuates oleic acid-induced acute lung injury via improving oxidative stress and inflammation.
کلیدواژه‌ها
Acute lung injury؛ Gene knockout؛ Inflammation؛ Oleic acid؛ Oxidative stress؛ Protein kinase C θ
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