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Miryounesi, Mohammad, Nayernia, Karim, Dianatpour, Mahdi, Mansouri, Fatemeh, Modarressi*, Mohammad Hossein. (1392). Co-culture of Mouse Embryonic Stem Cells with Sertoli Cells Promote in vitro Generation of Germ Cells. , 16(6), 779-783. doi: 10.22038/ijbms.2013.994
Mohammad Miryounesi; Karim Nayernia; Mahdi Dianatpour; Fatemeh Mansouri; Mohammad Hossein Modarressi*. "Co-culture of Mouse Embryonic Stem Cells with Sertoli Cells Promote in vitro Generation of Germ Cells". , 16, 6, 1392, 779-783. doi: 10.22038/ijbms.2013.994
Miryounesi, Mohammad, Nayernia, Karim, Dianatpour, Mahdi, Mansouri, Fatemeh, Modarressi*, Mohammad Hossein. (1392). 'Co-culture of Mouse Embryonic Stem Cells with Sertoli Cells Promote in vitro Generation of Germ Cells', , 16(6), pp. 779-783. doi: 10.22038/ijbms.2013.994
Miryounesi, Mohammad, Nayernia, Karim, Dianatpour, Mahdi, Mansouri, Fatemeh, Modarressi*, Mohammad Hossein. Co-culture of Mouse Embryonic Stem Cells with Sertoli Cells Promote in vitro Generation of Germ Cells. , 1392; 16(6): 779-783. doi: 10.22038/ijbms.2013.994

Co-culture of Mouse Embryonic Stem Cells with Sertoli Cells Promote in vitro Generation of Germ Cells

Iranian Journal of Basic Medical Sciences
مقاله 7، دوره 16، شماره 6، شهریور 2013، صفحه 779-783    اصل مقاله (516.27 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2013.994
نویسندگان
Mohammad Miryounesi1؛ Karim Nayernia2؛ Mahdi Dianatpour3؛ Fatemeh Mansouri1؛ Mohammad Hossein Modarressi*1
1Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
2GENEOCELL, Advanced Molecular & Cellular Technologies, Montreal, Canada
3Department of Medical Genetics, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
چکیده
 

Objective(s):
Sertoli cells support in vivo germ cell production; but, its exact mechanism has not been well understood. The present study was designed to analyze the effect of Sertoli cells in differentiation of mouse embryonic stem cells (mESCs) to germ cells.
 
Materials and Methods:
A fusion construct composed of a Stra8 gene promoter and the coding region of enhanced green fluorescence protein was produced to select differentiated mESCs. To analyze sertoli cells’ effect in differentiation process, mESCs were separated into two groups: the first group was cultured on gelatin with retinoic acid treatment and the second group was co-cultured with sertoli cell feeder without retinoic acid induction. Expressions of pre-meiotic (Stra8), meiotic (Dazl and Sycp3) and post-meiotic (Prm1) genes were evaluated at different differentiation stages (+7, +12 and +18 days of culture).
Results:
In the first group, expressions of meiotic and post-meiotic genes started 12 and 18 days after induction with retinoic acid, respectively. In the second group, 7 days after co-culturing with Sertoli cells, expression of meiotic and post-meiotic genes was observed.
Conclusion:
These results show that differentiation process to germ cells is supported by Sertoli cells. Our findings provide a novel effective approach for generation of germ cell in vitro and studying the interaction of germ cells with their niche.
کلیدواژه‌ها
Co-culture Differentiation Embryonic stem cell In vitro derived germ cells Sertoli cell
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