miR-451 Up-regulation, Induce Erythroid Differentiation of CD133+cells Independent of Cytokine Cocktails | ||
| Iranian Journal of Basic Medical Sciences | ||
| مقاله 4، دوره 16، شماره 6، شهریور 2013، صفحه 756-762 اصل مقاله (594.27 K) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22038/ijbms.2013.991 | ||
| نویسندگان | ||
| Fatemeh Kouhkan1؛ Masoud Soleimani* 2؛ Morteza Daliri3؛ Majid Mossahebi-mohammadi4؛ Shahin Mohammadi5؛ Mehdi Mokhtari6؛ Reyhaneh Lahmy1؛ naser Mobarra4؛ Mehrdad Behmanesh1 | ||
| 1Department of Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran | ||
| 2Department of Hematology, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran | ||
| 3National institute of genetic engineering and biotechnology, Tehran, Iran | ||
| 4Department of Clinical biochemistry, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran | ||
| 5Department of Hematology, Allied Medical School, Tehran University of Medical Sciences, Tehran, Iran | ||
| 6Departments of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran | ||
| چکیده | ||
| Objective(s): Erythropoiesis is regulated by some extrinsic and intrinsic factors as microRNAs (miRNAs). miRNAs are endogenously small non-coding regulatory RNAs which play vital roles in the variety of cellular fate, critical processes; growth, apoptosis, metabolism, survival of the cells and specially differentiation. Several miRNAs such as miR-16 and miR-451 have been shown to be correlated with erythroid differentiation. Taking into account the importance of miRNAs in cellular differentiation, the goal of the present study was to examine the role of miRNAs in hematopoietic stem cells (HSC) differentiation into the erythroid cells in the absence of growth factors and stimulatory cytokines. Materials and Methods: CD133+ stem cells were infected with lentiviruses containing miR-451/miR-16 precursor sequence, erythroid differentiation was evaluated using RT-PCR for hemoglobin chains and surface antigens, also by banzidine staining. Results: MiR-451up-regulation, but not miR-16, could induce α, β and γ-globin expression in CD133+ cells and have strong correlation with appearance of CD71 and CD235a markers in these cells. Moreover, miR-451 up-regulation increases the banzidine positive cells to ~ %40. Conclusion: Our results provide strong evidence that miR-451 up-regulation strongly induces erythroid differentiation and maturation of CD133+ stem cells. Hence, this method may provide a useful technique for the production of artificial blood RBC and be used as a new strategy for gene therapy of hemoglobinopathies, such as β-thalassemias and sickle cell anemia. | ||
| کلیدواژهها | ||
| Erythropoiesis CD133+ microRNA miR-451 miR-16 | ||
| مراجع | ||
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