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Bahreyni-Toossi, Mohammad Taghi, vosoughi, habibeh, Azimian, Hosein, Rezaei, Abdul Rahim, Momennezhad, Mahdi. (1396). In vivo Exposure Effects of 99mTc-methoxyisobutylisonitrile on the FDXR and XPA Genes Expression in Human Peripheral Blood Lymphocytes. , 6(1), 32-40. doi: 10.22038/aojnmb.2017.9678
Mohammad Taghi Bahreyni-Toossi; habibeh vosoughi; Hosein Azimian; Abdul Rahim Rezaei; Mahdi Momennezhad. "In vivo Exposure Effects of 99mTc-methoxyisobutylisonitrile on the FDXR and XPA Genes Expression in Human Peripheral Blood Lymphocytes". , 6, 1, 1396, 32-40. doi: 10.22038/aojnmb.2017.9678
Bahreyni-Toossi, Mohammad Taghi, vosoughi, habibeh, Azimian, Hosein, Rezaei, Abdul Rahim, Momennezhad, Mahdi. (1396). 'In vivo Exposure Effects of 99mTc-methoxyisobutylisonitrile on the FDXR and XPA Genes Expression in Human Peripheral Blood Lymphocytes', , 6(1), pp. 32-40. doi: 10.22038/aojnmb.2017.9678
Bahreyni-Toossi, Mohammad Taghi, vosoughi, habibeh, Azimian, Hosein, Rezaei, Abdul Rahim, Momennezhad, Mahdi. In vivo Exposure Effects of 99mTc-methoxyisobutylisonitrile on the FDXR and XPA Genes Expression in Human Peripheral Blood Lymphocytes. , 1396; 6(1): 32-40. doi: 10.22038/aojnmb.2017.9678

In vivo Exposure Effects of 99mTc-methoxyisobutylisonitrile on the FDXR and XPA Genes Expression in Human Peripheral Blood Lymphocytes

Asia Oceania Journal of Nuclear Medicine and Biology
مقاله 5، دوره 6، شماره 1، فروردین 2018، صفحه 32-40    اصل مقاله (775.43 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/aojnmb.2017.9678
نویسندگان
Mohammad Taghi Bahreyni-Toossi1؛ habibeh vosoughi* 2؛ Hosein Azimian3؛ Abdul Rahim Rezaei4؛ Mahdi Momennezhad5
1Medical Physics Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
2Department of Medical Physics, Faculty of Medicine, Azadi Square, Pardis Campus, Mashhad-Iran.
3Medical Physics Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
4Immunology Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
5Nuclear Medicine Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
چکیده
Objective(s): In recent years, the application of radiopharmaceuticals in nuclear medicine has increased substantially. Following the diagnostic procedures performed in nuclear medicine departments, such as myocardial perfusion imaging, patients generally receive considerable doses of radiation. Normally, radiation-induced DNA damages are expected following exposure to a low-dose ionizing radiation. In order to detect molecular changes, high-sensitivity techniques must be utilized. The aim of this study was to assess the effect of a low-dose (below 10 mSv) gamma ray on gene expression using quantitative real-time polymerase chain reaction (qRT-PCR). Methods: Blood samples were obtained from 20 volunteer patients who underwent myocardial perfusion imaging. They were given various doses of Technetium99-m methoxyisobutylisonitrile (99mTc-MIBI). After that, peripheral blood mononuclear cells (PBMNs) were derived, and then total RNA was extracted and reverse-transcribed to cDNA. Finally, the expression levels of xeroderma pigmentosum complementation group-A (XPA) and ferredoxin reductase (FDXR) genes were determinded through qRT-PCR technique using SYBR Green. Results: XPA and FDXR expression levels were obtained following a very low-dose ionizing radiation. A significant up-regulation of both genes was observed, and the gene expression level of each individual patient was different. If differences in the administered activity and radiosensitivity are taken into account, the observed differences could be justified. Furthermore, gender and age did not play a significant role in the expression levels of the genes under study. Conclusion: The up-regulation of FDXR after irradiation revealed the high-sensitivity level of this gene; therefore, it could be used as an appropriate biomarker for biological dosimetry. On the other hand, the up-regulation of XPA is an indication of DNA repair following radiation exposure. According to linear no-threshold model (LNT) and the results obtained from this study, a very low dose of ionizing radiation could bring about adverse biological effects at molecular level in the irradiated person.
کلیدواژه‌ها
Gene expression؛ Biodosimetry؛ DNA damage؛ human peripheral blood lymphocytes؛ Ionizing radiation
مراجع
 

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