Neuroprotection of a sesamin derivative, 1, 2-bis [(3-methoxy- phenyl) methyl] ethane-1, 2-dicaroxylic acid (MMEDA) against ischemic and hypoxic neuronal injury | ||
| Iranian Journal of Basic Medical Sciences | ||
| مقاله 6، دوره 20، شماره 12، اسفند 2017، صفحه 1324-1330 اصل مقاله (1.17 M) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22038/ijbms.2017.9543 | ||
| نویسندگان | ||
| Chang-Tsen Hung1؛ Li-Dian Chen2؛ Chien-Wei Hou* 3 | ||
| 1Department of Health and Leisure Management, Yuanpei University of Medical Technology, Hsinchu, Taiwan | ||
| 2Department of Convalescence Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou China | ||
| 3Department of Biotechnology and Pharmaceutical Technology, Yuanpei University of Medical Technology, Hsinchu, Taiwan | ||
| چکیده | ||
| Objective(s): Stroke may cause severe neuronal damage. The sesamin have been demonstrated to possess neuroprotection by its antioxidant and anti-inflammatory properties. One sesamin derivative was artificially composited, 1, 2-bis [(3-methoxyphenyl) methyl] ethane-1, 2-dicaroxylic acid (MMEDA) had been developed to study its antioxidative activity and neuroprotection. Materials and Methods: The infaction of Sprague Dawley (SD) rats and hypoxia models of BV-2 microglia or PC12 cells were investigated for in vivo and in vitro test respectively. Lipid peroxidation and reactive oxygen species (ROS), prostaglandin E2 (PGE2) and related signaling pathways from hypoxic cells were analyzed by ELISA or Western blot assay, respectively. Results: MMEDA showed a protective effect when given 90 min after the focal cerebral ischemia. The neuroprotection of MMEDA was further confirmed by attenuating ROS and PGE2 release from hypoxic BV-2 or PC12 cells. MMEDA significantly reduced hypoxia-induced JNK and caspase-3 (survival and apoptotic pathways) in PC12 cells. Conclusion: The neuroprotective effect of MMEDA on ischemia/hypoxia models was involved with its antioxidative activity and anti-inflammatory effects. These results suggest that MMEDA exert effective neuroprotection against ischemia/hypoxia injury. | ||
| کلیدواژهها | ||
| Anti-inflammatory؛ Cerebral ischemia؛ Hypoxia؛ Neuroprotection؛ Reactive Oxygen Species؛ Sesamin derivative | ||
| مراجع | ||
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1. Ishida I, Kubo H, Suzuki S, Suzuki T, Akashi S, Inoue K, et al. Hypoxia diminishes toll-like receptor 4 expression through reactive oxygen species generated by mitochondria in endothelial cells. J Immunol 2002; 15:2069-2075.
2. Koga Y, Fujita M, Tsuruta R, Koda Y, Nakahara T, Yagi T, et al. Urinary trypsin inhibitor suppresses excessive superoxide anion radical generation in blood, oxidative stress, early inflammation, and endothelial injury in forebrain ischemia/reperfusion rats. Neurol Res 2010; 32:925-932.
3. Tsuruta R, Fujita M, Ono T, Koda Y, Koga Y, Yamamoto T, et al. Hyperglycemia enhances excessive superoxide anion radical generation, oxidative stress, early inflammation, and endothelial injury in forebrain ischemia/reperfusion rats. Brain Res 2010; 1309:155-163.
4. Hou CW, Wu CC, Yang CH, Jeng KC. Protective effects of sesamin and sesamolin on murine BV-2 microglia cell line under hypoxia. Neuroscience Letters 2004; 367:10–13.
5. Hou CW, Huang HM, Tzen JT, Jeng KC. Protective effects of sesamin and sesamolin on hypoxic neuronal and PC12 cells. J Neurosci Res 2003; 74:123-133.
6. Khan MM, Ishrat T, Ahmad A, Hoda MN, Khan MB, Khuwaja G, et al. Sesaminattenuates behavioral, biochemical and histological alterations induced by reversible middle cerebral artery occlusion in the rats. Chem Biol Interact. 2010; 183:255-263.
7. Bush ML, Miyashiro JS, Ingram VM. Activation of a neurofilament kinase, a tau kinase, and a tau phosphatase by decreased ATP levels in nerve growth factor-differentiated PC-12 cells. Proc Natl Acad Sci USA 1995; 92:1861-1865.
8. Lu QR, Yuk D, Alberta JA, Zhu Z, Pawlitzky I, Chan J, et al. Sonic hedgehog--regulated oligodendrocyte lineage genes encoding bHLH proteins in the mammalian central nervous system. Neuron 2000; 25:317-329.
9. Chao CC, Hu S, Tsang M, Weatherbee J, Molitor TW, Anderson WR, et al. Effects of transforming growth factor-beta on murine astrocyte glutamine synthetase activity. Implications in neuronal injury. J Clin Invest 1992; 90:1786-1793.
10. Sun AY, Cheng JS. Neuroprotective effects of poly (ADP-ribose) polymerase inhibitors in transient focal cerebral ischemia of rats. Zhongguo Yao Li Xue Bao 1998; 19:104-108.
11. Hou RCW, Chen HL, Tzen JTC, Jeng KC. Effect of sesame antioxidants on LPS-induced NO production by BV2 microglial cells. Neuroreport 2003; 14:1815-1819.
12. Hou RCW, Chen YS, Chen CH, Chen YH, Jeng JC. Protective effect of 1,2,4-benzenetriol on LPS-induced NO production by BV2 microglial cells. J Biomed Sci 2006; 13:89–99.
13. Hamada N, Tanaka A, Fujita Y, Itoh T, Ono Y, Kitagawa Y, et al. Involvement of heme oxygenase-1 induction via Nrf2/ARE activation inprotectionagainst H2O2-induced PC12 cell death by a metabolite ofsesamincontained in sesame seeds. Bioorg Med Chem 2011; 19:1959-1965.
14. Barone FC, Irving EA, Ray AM, Lee JC, Kassis S, Kumar S, et al. a second-generation p38 mitogen-activated protein kinase inhibitor, reduces brain injury and neurological deficits in cerebral focal ischemia. J Pharmacol Exp Ther 2001; 296:312-321.
15. Kaminska B, Gozdz A, Zawadzka M, Ellert-Miklaszewska A, Lipko M. MAPK signal transduction underlying brain inflammation and gliosis as therapeutic target. Anat Rec(Hoboken) 2009; 292:1902-1913.
16. Zeng KW, Fu H, Liu GX, Wang XM. Icariin attenuates lipopolysaccharide-induced microglial activation and resultant death of neurons by inhibiting TAK1/IKK/NF-kappaB and JNK/p38 MAPK pathways. Int Immunopharmacol 2010; 10: 668-678.
17. Tabakman R, Jiang H, Levine RA, Kohen R, Lazarovici P. Apoptotic characteristics of cell death and the neuroprotective effect of homocarnosine on pheochromocytoma PC12 cells exposed to ischemia. J Neurosci Res 2004; 75: 499-507.
18. Ha SK, Moon E, Kim SY. Chrysin suppresses LPS-stimulated proinflammatory responses by blocking NF-κB and JNK activations in microglia cells. Neurosci Lett 2010; 485:143-147.
19. Liou SF, Hsu JH, Liang JC, Ke HJ, Chen IJ, Wu JR, et al. San-Huang-Xie-Xin-Tang protects cardiomyocytes against hypoxia/reoxygenation injury via inhibition of oxidative stress-induced apoptosis. J Nat Med 2012; 66:311-320.
20. Chiu PY, Chen N, Leong PK, Leung HY, Ko KM. Schisandrin B elicits a glutathione antioxidant response and protects against apoptosis via the redox-sensitive ERK/Nrf2 pathway in H9c2 cells. Mol Cell Biochem 2011; 350: 237-250.
21. Jamarkattel-Pandit N, Pandit NR, Kim MY, Park SH, Kim KS, Choi H, et al. Neuroprotective effect of defatted sesame seeds extract against in vitro and in vivo ischemicneuronal damage. Planta Med 2010; 76:20-26.
22. Fujimura N, Sumita S, Narimatsu E. Alteration in diaphragmatic contractility during septic peritonitis in rats: effect of polyethylene glycol-absorbed superoxide dismutase. Crit Care Med 2000; 28:2406-2414.
23. Shin MJ, Kim DW, Lee YP, Ahn EH, Jo HS, Kim DS, et al. Tat-glyoxalase protein inhibits against ischemic neuronal cell damage and ameliorates ischemic injury. Free Radic Biol Med 2013; 67C:195-210.
24. Haddad JJ, Land SC. Redox/ROS regulation of lipopolysaccharide-induced mitogen-activated protein kinase (MAPK) activation and MAPK-mediated TNF-alpha biosynthesis. Br J Pharmacol 2002; 135:520-536. | ||
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