Extract of mouse embryonic stem cells induces the expression of pluripotency genes in human adipose tissue-derived stem cells | ||
| Iranian Journal of Basic Medical Sciences | ||
| مقاله 4، دوره 20، شماره 11، بهمن 2017، صفحه 1200-1206 اصل مقاله (1.08 M) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22038/ijbms.2017.9464 | ||
| نویسندگان | ||
| Paria Motamen Salehi1، 2؛ Tahereh Foroutan2؛ Arash Javeri1؛ Masoumeh Fakhr Taha* 1 | ||
| 1Department of Stem Cells and Regenerative Medicine, Institute for Medical Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran | ||
| 2Department of Biology, Faculty of Basic Science, Kharazmi University, Tehran, Iran | ||
| چکیده | ||
| Objective(s): In some previous studies, the extract of embryonic carcinoma cells (ECCs) and embryonic stem cells (ESCs) have been used to reprogram somatic cells to more dedifferentiated state. The aim of this study was to investigate the effect of mouse ESCs extract on the expression of some pluripotency markers in human adipose tissue-derived stem cells (ADSCs). Materials and Methods: Human ADSCs were isolated from subcutaneous abdominal adipose tissue and characterized by flow cytometric analysis for the expression of some mesenchymal stem cell markers and adipogenic and osteogenic differentiation. Frequent freeze-thaw technique was used to prepare cytoplasmic extract of ESCs. Plasma membranes of the ADSCs were reversibly permeabilized by streptolysin-O (SLO). Then the permeabilized ADSCs were incubated with the ESC extract and cultured in resealing medium.After reprogramming, the expression of some pluripotency genes was evaluated by RT-PCR and quantitative real-time PCR (qPCR) analyses. Results: Third-passaged ADSCs showed a fibroblast-like morphology and expressed mesenchymal stem cell markers. They also showed adipogenic and osteogenic differentiation potential. QPCR analysis revealed a significant upregulation in the expression of some pluripotency genes including OCT4, SOX2, NANOG, REX1 and ESG1 in the reprogrammed ADSCs compared to the control group. Conclusion: These findings showed that mouse ESC extract can be used to induce reprogramming of human ADSCs. In fact, this method is applicable for reprogramming of human adult stem cells to a more pluripotent sate and may have a potential in regenerative medicine. | ||
| کلیدواژهها | ||
| Adipose tissue-derived stem cells؛ Dedifferentiation؛ Embryonic stem cells extract؛ Reprogramming | ||
| مراجع | ||
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