Detection of tetracycline resistance genes, aminoglycoside modifying enzymes, and coagulase gene typing of clinical isolates of Staphylococcus aureus in the Southwest of Iran | ||
| Iranian Journal of Basic Medical Sciences | ||
| مقاله 10، دوره 20، شماره 8، آبان 2017، صفحه 912-919 اصل مقاله (868.5 K) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22038/ijbms.2017.9114 | ||
| نویسندگان | ||
| Seyed Sajjad Khoramrooz* 1؛ Samin Alipoor Dolatabad2؛ Fatemeh Mostafapour Dolatabad3؛ Masoud Marashifard4؛ Mehdi Mirzaii5؛ Hossein Dabiri6؛ Azam Haddadi2؛ Seyed Mohammadreza Rabani7؛ Hamid Reza Ghaffarian Shirazi1؛ Davood Darban-Sarokhalil8 | ||
| 1Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran | ||
| 2Department of Biology, Faculty of Science, Karaj Branch, Islamic Azad University, Karaj, Iran | ||
| 3Department of Basic Sciences, Islamic Azad University, Yasooj Branch, Yasooj, Iran | ||
| 4Student Research Committee, Yasuj University of Medical Sciences, Yasuj, Iran | ||
| 5School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran | ||
| 6Department of Medical Microbiology, Shahid Beheshti University of Medical Sciences, Tehran, Iran | ||
| 7Beheshti Teaching Hospital, Yasuj University of Medical Sciences, Yasuj, Iran | ||
| 8Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran | ||
| چکیده | ||
| Objective(s): The aim of the present study was to determine the aminoglycoside modifying enzymes (AMEs) encoded genes, tetracycline resistance genes, and the coa based typing of Staphylococcus aureus isolates in the Southwest of Iran. Materials and Methods: Antimicrobial susceptibility of isolates was carried out by agar disk diffusion methods. Two sets of multiplex PCR mixture were used for detection of AME genes and tet genes. All of the isolates were typed with the coagulase gene typing method. Of the 121 isolates, 29.75% and 47.93% were resistant to at least one aminoglycosides and tetracyclines, respectively. Results: The aac(6')-Ie-aph(2'') was the most frequent gene (97.22%), and aph (3')-IIIa and ant (4')-Ia genes were detected in 61.11% and 11.11% of aminoglycoside resistant isolates, respectively. The tetK and tetM genes were detected in 82.75% and 56.9% of tetracycline resistant isolates, respectively. Overall 31.4% of isolates were MRSA. Totally 17 distinct coa gene RFLP patterns, numbered C1 to C17, were observed. The C5 was the most frequent coa type with 31 isolates. Conclusion: The aac(6')-Ie-aph(2'') and aph (3')-IIIa genes were the most important genes contributing to aminoglycosides resistance, while resistance to tetracyclines was mediated by tetK and tetM genes. Interestingly all S. aureus with C5 as the most prevalent coa-type were resistant to at least one of the aminoglycoside antibiotics and tetracycline simultaneously. Moreover, 30 out of 31 isolates with this coa type were MRSA, indicating the importance of the C5 coa-type in MRSA strains and also in isolates that were resistant to aminoglycosides and tetracycline. | ||
| کلیدواژهها | ||
| Aminoglycoside resistance؛ Aminoglycoside modifying enzymes؛ Coagulase gene typing؛ Staphylococcus aureus؛ Tetracycline resistance | ||
| مراجع | ||
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