Investigation of the relationship between the Th17/IL-23 pathway and innate-adaptive immune system in TNBS-induced colitis in rats | ||
| Iranian Journal of Basic Medical Sciences | ||
| مقاله 5، دوره 20، شماره 8، آبان 2017، صفحه 870-879 اصل مقاله (1.76 M) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22038/ijbms.2017.9108 | ||
| نویسندگان | ||
| İhsan Karaboga1؛ Selim Demirtas2؛ Turan Karaca* 2 | ||
| 1Namik Kemal University, School of Health, 59030, Tekirdag, Turkey | ||
| 2University of Trakya, Faculty of Medicine, Department of Histology- Embryology, Balkan Campus, 22030, Edirne, Turkey | ||
| چکیده | ||
| Objective(s): This study was aimed at investigating immune activations of the 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced colitis model in colonic mucosa by immunohistochemical and Western blot methods. Materials and Methods: For this purpose, 16 female Wistar albino rats were divided into two random groups of control (n=8) and colitis (n=8). The experimental colitis model was induced by intracolonic administration of TNBS (25 mg/rat). Control animals received only rectal saline for the same time. The animals were sacrificed on the 15th day after TNBS administration, and colon tissue was removed and examined morphologically. Colon samples were stained immunohistochemically with anti-CD3, anti-CD4, anti-CD5, anti-CD8, anti-CD11b, anti-CD45, anti-TNF-α, anti-IL-17, anti-IL-22 and anti-IL-23 antibodies. Additionally, the colonic tissue IL-17 and IL-22 expressions were examined by the Western blot method. Results: In the experimental results, it was determined that there was a significant decrease in body weight and an increase in colon weight in the colitis group when comparing initial experiments. The colon tissue ulcerations, inflammation, crypt loss and Goblet cell loss were observed in the colitis group in microscopic examinations. The immunohistochemical positive cell numbers significantly increased in the colitis group. The immunoreactive lymphocytes in the propria, intracryptal and submucosal layers were found to be increased in the colitis group of rats. In addition, IL-17 and IL-23 expressions were increased in colitis colon mucosa found by Western blot analysis. Conclusion: The Th17/IL-23 pathway and IL-22 serve important roles in the pathogenesis of ulcerative colitis, and will be further examined by study. | ||
| کلیدواژهها | ||
| immunohistochemistry؛ Rat؛ 2,4,6-trinitrobenzene sulfonic acid؛ Ulcerative colitis؛ Western blot | ||
| مراجع | ||
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