Frequency of bap and cpaA virulence genes in drug resistant clinical isolates of Acinetobacter baumannii and their role in biofilm formation | ||
| Iranian Journal of Basic Medical Sciences | ||
| مقاله 2، دوره 20، شماره 8، آبان 2017، صفحه 849-855 اصل مقاله (1.07 M) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22038/ijbms.2017.9105 | ||
| نویسندگان | ||
| Arezoo Fallah1، 2؛ Mohammad Ahangarzadeh Rezaee* 1، 3؛ Alka Hasani3؛ Mohammad Hossein Soroush Barhaghi4؛ Hossein Samadi Kafil5 | ||
| 1Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran | ||
| 2Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran | ||
| 3Infectious and Tropical Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran | ||
| 4Department of Microbiology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran | ||
| 5Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran | ||
| چکیده | ||
| Objective(s): Acinetobacter baumannii has a high propensity to form biofilm and frequently causes medical device-related infections with multiple-drug-resistance in hospitals. The aim of this work is to study antimicrobial resistance and the role of bap and cpaA genes in biofilm formation by A. baumannii to understand how this pathogen persists in the hospital environment. Materials and Methods: Theantibiotic resistance profile and in vitro biofilm-forming ability of one hundred clinical isolates of A. baumannii was evaluated by disc diffusion and crystal-violet staining methods, respectively. Isolates were tested for the presence of bap and cpaA genes. Results: The isolates were highly resistant to cefepime, third-generation cephalosporins, ciprofloxacin, cotrimoxazole, aminoglycosides and carbapenems. Moreover, four isolates were resistant to colistin. Quantification of biofilm showed that 43% of the isolates were strong biofilm-producer. Furthermore, 32% of the isolates exhibited moderate biofilm-formation and showed initial binding activity. Frequency of bap and cpaA were determined 92% and 36%, respectively. Conclusion: There was strong association between the presence of bap gene and biofilm formation by A. baumannii isolates (P=0.003). In addition, multidrug resistant isolates produced stronger biofilm than other isolates (P=0.0001). These results indicate importance of biofilm in resistance of isolates and effect of presence of bap gene in biofilm formation by A. baumannii strains. | ||
| کلیدواژهها | ||
| Acinetobacter baumannii؛ Antibiotic resistance؛ Biofilm؛ Bap؛ CpaA | ||
| مراجع | ||
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