Liu, Zhenzhen, Pan, Ning, Lv, Xiangwei, Li, Song, Wang, Liming, Liu, Qinlong. (1396). Orthotopic liver transplantation from cardiac death donors in the mouse: a new model and evaluation of cardiac death time. , 20(6), 683-689. doi: 10.22038/ijbms.2017.8838
Zhenzhen Liu; Ning Pan; Xiangwei Lv; Song Li; Liming Wang; Qinlong Liu. "Orthotopic liver transplantation from cardiac death donors in the mouse: a new model and evaluation of cardiac death time". , 20, 6, 1396, 683-689. doi: 10.22038/ijbms.2017.8838
Liu, Zhenzhen, Pan, Ning, Lv, Xiangwei, Li, Song, Wang, Liming, Liu, Qinlong. (1396). 'Orthotopic liver transplantation from cardiac death donors in the mouse: a new model and evaluation of cardiac death time', , 20(6), pp. 683-689. doi: 10.22038/ijbms.2017.8838
Liu, Zhenzhen, Pan, Ning, Lv, Xiangwei, Li, Song, Wang, Liming, Liu, Qinlong. Orthotopic liver transplantation from cardiac death donors in the mouse: a new model and evaluation of cardiac death time. , 1396; 20(6): 683-689. doi: 10.22038/ijbms.2017.8838

Orthotopic liver transplantation from cardiac death donors in the mouse: a new model and evaluation of cardiac death time

Iranian Journal of Basic Medical Sciences
مقاله 10، دوره 20، شماره 6، 2017، صفحه 683-689    اصل مقاله (1.34 M)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2017.8838
نویسندگان
Zhenzhen Liu1، 2؛ Ning Pan3؛ Xiangwei Lv4؛ Song Li1؛ Liming Wang1؛ Qinlong Liu* 1
1Department of General Surgery, The Second Affiliated Hospital of Dalian Medical University, Dalian, Liaoning Province, China
2Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), Peking University Cancer Hospital and Institute, Beijing, China
3Department of Anesthesiology, The Second Affiliated Hospital of Dalian Medical University, Dalian, Liaoning Province, China
4Department of General Surgery, Anhui Provincial Hospital, Hefei, Anhui, China
چکیده
Objective(s): The goal of this research was to develop a mouse orthotopic liver transplantation (LTx) model from donor-after-cardiac-death (DCD) grafts.
Materials and Methods: Mice were randomly assigned to the experimental group or the sham group. The mice in the experimental group were divided into three groups according to the warm ischemia time (WIT) of liver graft: normal LTx, WIT 30 minute (min) +LTx and WIT 45 min +LTx. The descending aorta was clamped using a miniature aortic clamp to simulate cardiac arrest in the DCD grafts. Subsequently, the grafts were orthotopically transplanted into C57BL/6 mice. The 7-day survival rate, serum alanine aminotransferase (ALT), inducible nitric oxide synthase (iNOS), interleukin-6 (IL-6) mRNA level, tumor necrosis factor-alpha (TNF-α) mRNA level, as well as hepatic pathologic alterations were observed.
Results: The 7-day survival rate was markedly lower in the WIT 45 min+LTx group than that in the normal LTx group (25% versus 100%, P-value<0.05), with no significant difference between the WIT 30 min +LTx and normal LTx group (75% versus 100%, P-value>0.05). Serum ALT level of WIT 45 min+LTx group was markedly higher than that of normal LTx and WIT 30 min+LTx group (P-value<0.01). There were significant differences in necrosis and apoptosis among the three groups (P-value<0.05). The expression of iNOS, IL-6 mRNA and TNF-α mRNA in WIT 45 min +LTx group all increased significantly compared with the normal LTx and WIT 30 min+LTx group.
Conclusion: The DCD LTx model is feasible in the mouse and would provide many advantages for biomedical research on LTx from DCD grafts.
کلیدواژه‌ها
Animal model؛ Liver transplantation؛ Primary graft dysfunction؛ Reperfusion injury؛ Warm ischemia time
مراجع
آمار
تعداد مشاهده مقاله: 1,379
تعداد دریافت فایل اصل مقاله: 1,207
دانشگاه علوم پزشکی مشهد
آمار
تعداد نشریات29
تعداد شماره‌ها2,318
تعداد مقالات24,803
تعداد مشاهده مقاله82,156,996
تعداد دریافت فایل اصل مقاله45,359,744