The effects of ethanol extract of Berberis vulgaris fruit on histopathological changes and biochemical markers of the liver damage in diabetic rats | ||
| Iranian Journal of Basic Medical Sciences | ||
| مقاله 13، دوره 20، شماره 5، مرداد 2017، صفحه 552-556 اصل مقاله (971.38 K) | ||
| شناسه دیجیتال (DOI): 10.22038/ijbms.2017.8681 | ||
| نویسندگان | ||
| Mohammad Rahimi-Madiseh1؛ Paridokht Karimian2؛ Marzieh Kafeshani3؛ Mahmoud Rafieian-Kopaei* 1 | ||
| 1Medical Plants Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran | ||
| 2Department of Pathology, Shahrekord University of Medical Sciences, Shahrekord, Iran | ||
| 3Department of Clinical Nutrition/Community Nutrition/Food Science & Technology, Food Security Research Center, School of Nutrition & Food Science, Isfahan, Iran | ||
| چکیده | ||
| Objective(s): Various studies have shown that the diabetes is associated with liver failure. The objective of this study was determining the effects of Berberis vulgaris fruit on histopathological and biochemical markers of liver in diabetic rats. Materials and Methods: In this experimental study, 60 male Wistar rats weighing 200-250 g with free access to water and ad libitum were randomly divided to five twelve-membered groups including healthy control (group 1), diabetic control (group 2, this two groups received distilled water), treated diabetic positive control (group 3) using dose 150 mg/kg/day metformin, and two groups treated with doses 200 (group 4) and 600 (group 5) mg/kg/BW of B. vulgaris extracts via gavage feeding for 8 weeks. Diabetes mellitus was experimentally induced by one dose injection of alloxan 120 mg/kg. This pre-clinical study was performed on 120 mg/kg alloxan induced diabetic rats. Results: The hepatic steatosis status, liver cholestasis and fibrosis were not changed in group 4 and 5. Glycogen deposition changed mildly and polymorphonuclear neutrophils infiltration changed moderately at group 5. Liver hepatitis changed mildly and severely at group 3 as well as group 5, respectively. Glucose, serum glutamic-oxaloacetic transaminase, serum glutamic pyruvic transaminase, and alkaline phosphatase were lower in high dose group compared to other groups. Conclusion: Results suggested that B. vulgaris extract can decrease liver damage by influencing hepatic histopathological and biochemical markers in diabetic rats. | ||
| کلیدواژهها | ||
| Berberine؛ Biochemical markers؛ Diabetes؛ Histopathology؛ Herbal Medicine؛ Liver diseases | ||
| مراجع | ||
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1. Nasri H, Rafieian-Kopaei M. Metformin and diabetic kidney disease: a mini-review on recent findings. Iran J Pediatr 2014; 24:565.
2. Nasri H, Rafieian-Kopaei M. Effect of vitamin D on insulin resistance and nephropathy in type 2 diabetes. J Res Med Sci 2014; 19:581-582.
3. Rafieian-Kopaei M, Behradmanesh S, Kheiri S, Nasri H. Association of serum uric acid with level of blood pressure in type 2 diabetic patients. Iran J kid Dis 2014; 8:152.
4. Baradaran A, Madihi Y, Merrikhi A, Rafieian-Kopaei M, Nasri H. Serum lipoprotein (a) in diabetic patients with various renal function not yet on dialysis. Pak J Med Sci 2013; 29: 354-357.
5. Nasri H, Rafieian-Kopaei M. Comment on: The effects of green tea consumption on metabolic and anthropometric indices in patients with type 2 diabetes. J Res Med Sci 2014; 19:1203-1204.
6. Nasri H, Shirzad H, Baradaran A, Rafieian-kopaei M. Antioxidant plants and diabetes mellitus. J Res Med Sci 2015; 20:491.
7. Bahmani M, Zargaran A, Rafieian-Kopaei M, Saki K. Ethnobotanical study of medicinal plants used in the management of diabetes mellitus in the Urmia, Northwest Iran. Asian Pac J Trop Med 2014; 7:S348-S354.
8. Bahmani M, Sarrafchi A, Shirzad H, Rafieian-Kopaei M. Autism: pathophysiology and promising herbal remedies. Curr Pharm Des 2016; 22:277-285.
9. Sarrafchi A, Bahmani M, Shirzad H, Rafieian-Kopaei M. Oxidative stress and parkinson’s disease: new hopes in treatment with herbal antioxidants. Curr Pharm Des 2016; 22:238-246.
10. Shayganni E, Bahmani M, Asgary S, Rafieian-Kopaei M. Inflammaging and cardiovascular disease: Management by medicinal plants. Phytomedicine 2015; 23:1119-1126.
11. Nasri H, Ardalan MR, Rafieian-Kopaei M. Mechanistic impacts of medicinal plants in diabetic kidney disease. Iran J Public Health 2014; 43:1311–1313.
12. Nasri H, Rafieian-Kopaei M. Protective effects of herbal antioxidants on diabetic kidney disease. J Res Med Sci 2014; 19; 19:82-83.
13. Kafeshani M. Ginger, micro-inflammation and kidney disease. J Renal Endocrinol 2015;1:e04.
14. al AE-We. In vitro biological assessment of berberis vulgaris and its active constituent, berberine: antioxidants, anti-acetylcholinesterase, anti-diabetic and anticancer effects. BMC Complement Altern Med 2013; 13:218.
15. Iloon Kashkooli R, Najafi SS, Sharif F, Hamedi A, Hoseini Asl MK, Najafi Kalyani M, et al. The effect of Berberis vulgaris extract on transaminase activities in non-alcoholic fatty liver disease. Hepat Mont 2015; 15:e25067.
16. Shidfar F, Seyed Ebrahimi S, Hosseini S, Heydari I, Shidfar S, Hajhassani G. The effects of Berberis vulgaris fruit extract on serum lipoproteins, apoB, apoA-I, homocysteine, glycemic control and total antioxidant capacity in type 2 diabetic patients. Iran J Pharm Res 2012; 11:643-652.
17. Girish C, Koner BC, Jayanthi S, Rao KR, Rajesh B, Pradhan SC. Hepatoprotective activity of six polyherbal formulations in CCl4-induced liver toxicity in mice. Indian J Exp Biol 2009; 47:257.
18. Upwar N, Patel R, Waseem N, Mahobia NK. Hypoglycemic effect of methanolic extract of Berberis aristata DC stem on normal and streptozotocin induced diabetic rats. Int J Pharm Pharm Sci 2011; 3:222-224.
19. Meliani N, Dib MEA, Allali H, Tabti B. Hypoglycaemic effect of Berberis vulgaris L. in normal and streptozotocin-induced diabetic rats. Asian Pac J Trop Biomed 2011; 1:468-471.
20. Rafieian-Kopaei M, Nasri H. The ameliorative effect of Zingiber officinale in diabetic nephropathy. Iran Red Crescent Med J 2014;16: e11324.
21. Rafieian-Kopaei M, Nasri H, Baradaran A. Teucrium polium: Liver and kidney effects. J Res Med Sci 2014; 19:478-479.
22. Rafieian-Kopaei M, Setorki M, Heidarian E, Shahinfard N, Ansari R. Effect of Anethum graveolens on hypelipidemia induced hepatotoxicity. Toxicol Lett 2012; 211:S167.
23. Heidarian E, Rafieian-Kopaei M. Amelioration of lead toxicity from rat liver with artichoke leaf extract. Toxicol Lett 2012; 211:S142.
24. Namjoo A, Nasri H, Talebi-Juneghani A, Baradaran A, Rafieian-Kopaei M. Safety profile of Carthamus tinctorius L. in lactation: brain, renal and hepatotoxicity 2013;29:378-383.
25. Othman MS, Safwat G, Aboulkhair M, Abdel Moneim AE. The potential effect of berberine in mercury-induced hepatorenal toxicity in albino rats. Food Chem Toxicol 2014; 69:175-181.
26. Hermenean A, Popescu C, Ardelean A, Stan M, Hadaruga N, Mihali CV, et al. Hepatoprotective effects of Berberis vulgaris L. extract/beta cyclodextrin on carbon tetrachloride-induced acute toxicity in mice. Int J Mol Sci 2012; 13:9014-9034.
27. Ashraf H, Zare S. Preventive effects of aqueous extract of Berberis integerrima Bge. Root on liver injury induced by diabetes mellitus (type 1) in rats. Iran J Pharm Res 2015; 14:335-343.
28. Murugeh S K, Murugeh K S, Veerendra C Y, Bhim C M, Tapan K M. Hepato protective and antioxidant role of Berberis tinctoria lesch leaves on paracetamol induced hepatic damage in rats. Iran J Pharm Ther 2005; 4:64-69.
29. DuPont RL, Rice DP, Miller LS, Shiraki SS, Rowland CR, Harwood HJ. Economic costs of anxiety disorders. Anxiety 1996; 2:167-172.
30. Nasri H, Rafieian-Kopaei M. Tubular kidney protection by antioxidants. Iran J Public Health 2013; 42:1194-1196.
31. Baradaran A, Nasri H, Rafieian-Kopaei M. Protection of renal tubular cells by antioxidants: current knowledge and new trends. Cell J 2015; 16:568-571.
32. Rafieian-Kopaei M, Nasri H. Re: Erythropoietin ameliorates oxidative stress and tissue injury following renal ischemia/reperfusion in rat kidney and lung. Med Princ Pract 2014;23:95.
33. Baradaran A, Rafieian-kopaei M. Oxidative stress and hypertension: Possibility of hypertension therapy with antioxidants. J Res Med Sci 2014; 19:358-367.
34. Shirzad H, Taji F, Rafieian-Kopaei M. Correlation between antioxidant activity of garlic extracts and WEHI-164 fibrosarcoma tumor growth in BALB/c mice. J Med Food 2011; 14:969-974.
35. Nasri H, Rafieian-Kopaei M. Medicinal plants and antioxidants: why they are not always beneficial? Iran J Public Health 2014; 43:255.
36. Sharafati Chaleshtori R, Rokni N, Rafieian-Kopaei M, Drees F, Salehi E. Antioxidant and antibacterial activity of Basil (Ocimum basilicum L.) Essential oil in beef burger. J Agric Sci Technol 2015; 17:817-826.
37. Rafieian-Kopaei M. In vitro evaluation of antioxidant properties of ten Iranian medicinal plants. Iran Red Crescent Med J 2014; 16:e10264.
38. Cicero AF, Baggioni A. Berberine and its role in chronic disease. Adv Exp Med Biol 2016; 928:27-45.
39. Shidfar F, Ebrahimi SS, Hosseini S, Heydari I, Shidfar S, Hajhassani G. The effects of Berberis vulgaris fruit extract on serum lipoproteins, apoB, apoA-I, homocysteine, glycemic control and total antioxidant capacity in type 2 diabetic patients. Iran J Pharm Res 2012; 11:643-652. | ||
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