Mohammadi, Saeed, Seyedhoseini, Fakhri Sadat, Asadi, Jahanbakhsh, Yazdani, Yaghoub. (1396). Effects of berberine on the secretion of cytokines and expression of genes involved in cell cycle regulation in THP-1 monocytic cell line. , 20(5), 530-537. doi: 10.22038/ijbms.2017.8677
Saeed Mohammadi; Fakhri Sadat Seyedhoseini; Jahanbakhsh Asadi; Yaghoub Yazdani. "Effects of berberine on the secretion of cytokines and expression of genes involved in cell cycle regulation in THP-1 monocytic cell line". , 20, 5, 1396, 530-537. doi: 10.22038/ijbms.2017.8677
Mohammadi, Saeed, Seyedhoseini, Fakhri Sadat, Asadi, Jahanbakhsh, Yazdani, Yaghoub. (1396). 'Effects of berberine on the secretion of cytokines and expression of genes involved in cell cycle regulation in THP-1 monocytic cell line', , 20(5), pp. 530-537. doi: 10.22038/ijbms.2017.8677
Mohammadi, Saeed, Seyedhoseini, Fakhri Sadat, Asadi, Jahanbakhsh, Yazdani, Yaghoub. Effects of berberine on the secretion of cytokines and expression of genes involved in cell cycle regulation in THP-1 monocytic cell line. , 1396; 20(5): 530-537. doi: 10.22038/ijbms.2017.8677

Effects of berberine on the secretion of cytokines and expression of genes involved in cell cycle regulation in THP-1 monocytic cell line

Iranian Journal of Basic Medical Sciences
مقاله 11، دوره 20، شماره 5، 2017، صفحه 530-537    اصل مقاله (1.18 M)
شناسه دیجیتال (DOI): 10.22038/ijbms.2017.8677
نویسندگان
Saeed Mohammadi1؛ Fakhri Sadat Seyedhoseini2؛ Jahanbakhsh Asadi3؛ Yaghoub Yazdani* 2
1Student Research Committee and Department of Molecular Medicine, School of Advanced Technologies in Medicine, Golestan University of Medical Sciences, Gorgan, Iran
2Infectious Diseases Research Center and Laboratory Science Research Center, Golestan University of Medical Sciences, Gorgan, Iran
3Department of Clinical Biochemistry, Faculty of Medicine, Golestan University of Medical Sciences, Gorgan, Iran
چکیده
Objective(s): Current acute myeloid leukemia (AML) therapeutic strategies have irreversible side-effects. Berberine (BBR) is an isoquinoline alkaloid, which has been known as an aryl hydrocarbon receptor (AhR) ligand. AhR is a cytoplasmic receptor, which is involved in the regulation of cellular and immune responses. Here, we investigated the expression profile of genes involved in the cell cycle and different cytokines upon BBR-mediated AhR activation on AML THP-1 cell line.
Materials and Methods: THP-1 cells and normal monocytes were treated with different concentrations of BBR (10 μM, 25 μM, 50 μM, and 100 μM) for 24 and 48 hr. The cell viability was measured by MTT assay. Real-time RT-PCR was conducted to evaluate the expression of AhR, cytochrome P450 1A1 (CYP1A1), interleukin 1 beta (IL1β), p21, p27, cyclin-dependent kinase 2 (CDK2) and p53. Cellular expression of AhR was also assessed using immunofluorescence method. ELISA was used to determine the level of IL-10 and IL-12 cytokines.
Results: BBR inhibits the proliferation of THP-1 cells in a dose- and time-dependent manner with minimal toxicity on normal monocytes. Phorbol 12-myristate 13-acetate (PMA) treatment increased the cellular expression of AhR. The AhR target genes (CYP1A1, IL1β) were overexpressed upon BBR treatment. BBR downregulated Cdk2 and upregulated p21, p27 and p53 genes in THP-1 cells. IL-10 was significantly increased upon BBR treatment, while IL-12 was not significantly changed in all combinations.
Conclusion: BBR could be introduced as an effective chemotherapeutic agent against AML by giving rise to the expression of CDK inhibitors and anti-inflammatory cytokines and downregulation of CDK2.
کلیدواژه‌ها
Acute myeloid leukemia؛ Aryl hydrocarbon receptor؛ Berberine؛ Cell cycle regulation؛ THP-1
مراجع
آمار
تعداد مشاهده مقاله: 1,984
تعداد دریافت فایل اصل مقاله: 1,600
دانشگاه علوم پزشکی مشهد
آمار
تعداد نشریات29
تعداد شماره‌ها2,325
تعداد مقالات24,885
تعداد مشاهده مقاله83,068,839
تعداد دریافت فایل اصل مقاله46,060,690
اخبار نشریات