Zhao, Hong, Liu, Fei, Jia, Ruichun, Chang, Huiying, Li, Haixia, Miao, Meijuan, Wang, Hui, Yang, Zhiping. (1396). MiR-570 inhibits cell proliferation and glucose metabolism by targeting IRS1 and IRS2 in human chronic myelogenous leukemia. , 20(5), 481-488. doi: 10.22038/ijbms.2017.8671
Hong Zhao; Fei Liu; Ruichun Jia; Huiying Chang; Haixia Li; Meijuan Miao; Hui Wang; Zhiping Yang. "MiR-570 inhibits cell proliferation and glucose metabolism by targeting IRS1 and IRS2 in human chronic myelogenous leukemia". , 20, 5, 1396, 481-488. doi: 10.22038/ijbms.2017.8671
Zhao, Hong, Liu, Fei, Jia, Ruichun, Chang, Huiying, Li, Haixia, Miao, Meijuan, Wang, Hui, Yang, Zhiping. (1396). 'MiR-570 inhibits cell proliferation and glucose metabolism by targeting IRS1 and IRS2 in human chronic myelogenous leukemia', , 20(5), pp. 481-488. doi: 10.22038/ijbms.2017.8671
Zhao, Hong, Liu, Fei, Jia, Ruichun, Chang, Huiying, Li, Haixia, Miao, Meijuan, Wang, Hui, Yang, Zhiping. MiR-570 inhibits cell proliferation and glucose metabolism by targeting IRS1 and IRS2 in human chronic myelogenous leukemia. , 1396; 20(5): 481-488. doi: 10.22038/ijbms.2017.8671

MiR-570 inhibits cell proliferation and glucose metabolism by targeting IRS1 and IRS2 in human chronic myelogenous leukemia

Iranian Journal of Basic Medical Sciences
مقاله 5، دوره 20، شماره 5، 2017، صفحه 481-488    اصل مقاله (1.28 M)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2017.8671
نویسندگان
Hong Zhao1؛ Fei Liu1؛ Ruichun Jia1؛ Huiying Chang2؛ Haixia Li3؛ Meijuan Miao1؛ Hui Wang1؛ Zhiping Yang* 1
1Department of Blood Transfusion, the Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, China
2Department of Cardiology, the Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, China
3Department of Ultrasonography, Harbin medical university cancer hospital, Harbin, Heilongjiang 150081, China
چکیده
Objective(s): Chronic myelogenous leukemia (CML) is a chronic myeloproliferative disorder characterized by the accumulation of myeloid cells with a chromosomal translocation known as the Philadelphia chromosome. In this study, we investigated the roles of miR-570 in CML development.
Materials and Methods: Expression of miR-570 in CML samples and cell lines was determined by qRT-PCR. Glucose uptake and ATP concentration detection assays were used to analyze cell glucose metabolism. MTT and western blot assays were performed for cell proliferation and apoptosis, respectively. The targets of miR-570 were predicted by bioinformatics and confirmed using luciferase activity, qRT-PCR and western blot assays.
Results: The expression levels of miR-570 were significantly reduced in CML clinical samples and cells. Overexpression of miR-570 inhibited cell proliferation, promoted apoptosis, and suppressed glucose metabolism in CML cells. Insulin receptor substrates (IRS) 1 and IRS2 were identified as direct targets of miR-570. IRS1 or IRS2 were knocked down in K562 cells.Loss of IRS1/2 expression led to suppressed cell proliferation, elevated apoptosis, and decreased glucose metabolism in CML cells, which is consistent with their roles as miR-570 targets.
Conclusion: MiR-570 directly targeted IRS1 and IRS2 in CML, suppressing cell proliferation and glucose metabolism. MiR-570 may provide a strategy for CML therapy.
کلیدواژه‌ها
Chronic myelogenous leukemia؛ IRS1؛ IRS2؛ miR-570
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