Hydro-alcoholic extract of Matricaria recutita exhibited dual anti-spasmodic effect via modulation of Ca2+ channels, NO and PKA2-kinase pathway in rabbit jejunum | ||
| Avicenna Journal of Phytomedicine | ||
| مقاله 5، دوره 7، شماره 4، مهر و آبان 2017، صفحه 334-344 اصل مقاله (790.89 K) | ||
| نوع مقاله: Original Research Article | ||
| شناسه دیجیتال (DOI): 10.22038/ajp.2017.8607 | ||
| نویسندگان | ||
| Hamidreza yazdi1؛ Akhtar Seifi1؛ Shima Changizi1؛ Vahid Khori* 2؛ Fatemeh Hossini1؛ Ali Davarian1؛ Yahya Jand1؛ Ayesheh Enayati1؛ Masumeh Mazandarani3؛ Fateme Nanvabashi1 | ||
| 1Ischemic Disorders Research Center, Golestan University of Medical Sciences, Gorgan, Iran | ||
| 2Ischemic Disorders Research Center, Golestan University of Medical Sciences, Gorgan, Iran. | ||
| 3Department of Botany, Islamic Azad University, Gorgan, Iran | ||
| چکیده | ||
| Objective: Several studies have shown the antispasmodic activity of Matricariarecutita without detailing the underlying mechanism(s). The present study was designed to determine whether the antispasmodic mechanisms of M. recutita extract mediated via histaminergic/cholinergic receptors, Ca2+channels, activation of PKA2 and NO release in isolated rabbit jejunum. Methods and Materials: The concentration- dependent (3 × 10-3–1.3 × 10-2 mg/ml) antispasmodic effect of the hydro-alcoholic extract of M. recutita flowers was studied in isolated rabbit jejunum. The isolated jejunum preparations were divided into seven groups, including the pharmacological probes that modulate cholinergic, histaminergic, and nitrergic receptors, as well as PKA2. Results: M. recutita inhibited spontaneous smooth muscle contractility of the jejunum in a concentration-dependent manner (3 × 10-3–1.3 × 10-2 mg/ml) and reduced both K+- and Ca2+-induced contractions, which is similar to the effect of verapamil. The antispasmodic effect of M. recutita wasinhibited by H89 (a PKA2 inhibitor). The myorelaxant effect of M. recutita increased in the presence of ACh/His and H89. Conclusion: M. recutita evoked antispasmodic and spasmolytic effects mediated through different signaling pathways. Our results have shown this dual inhibitory effect is mediated by blocking Ca2+ channels, activating His and ACh receptors, releasing NO, and activating PKA2. | ||
| کلیدواژهها | ||
| Matricaria؛ Cholinergic Agents؛ Histamine Agents؛ Jejunum؛ Rabbit؛ Nitric oxide | ||
| مراجع | ||
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