Effects of pomegranate seed oil on oxidant/antioxidant balance in heart and kidney homogenates and mitochondria of diabetic rats and high glucose-treated H9c2 cell line | ||
| Avicenna Journal of Phytomedicine | ||
| مقاله 4، دوره 7، شماره 4، مهر و آبان 2017، صفحه 317-333 اصل مقاله (1.69 M) | ||
| نوع مقاله: Original Research Article | ||
| شناسه دیجیتال (DOI): 10.22038/ajp.2017.20252.1772 | ||
| نویسندگان | ||
| Hamid Mollazadeh1؛ Mohammad Taher Boroushaki2؛ Mohammad Soukhtanloo3؛ Amir Reza Afshari2؛ Mohammad Mahdi Vahedi* 4 | ||
| 1Department of physiology and pharmacology, School of Medicine, North Khorasan University of Medical Sciences, Bojnurd, Iran | ||
| 2Department of Pharmacology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran | ||
| 3Department of Biochemistry, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran. | ||
| 4department of pharmacology faculty of medicine, Mashhad university of medical sciences,Mashhad,Iran | ||
| چکیده | ||
| Objective: Oxidative stress is a major cause of diabetes complications. The present study aimed to investigate the beneficial effects of Pomegranate Seed Oil (PSO) on diabetes-induced changes in oxidant/antioxidant balance of the kidney, heart and mitochondria from rats and H9c2 cell line. Materials and Methods: In these in vivo and in vitro studies, male rats were divided into four groups (twelve each): group 1 served as control, group 2-4 received a single dose of streptozotocin (60 mg/kg, i. p), groups 3 and 4 received PSO (0.36 and 0.72 mg/kg/daily, gavage), respectively. After three weeks, six rats of each group and one week later the remaining animals were anaesthetized and the hearts and kidneys were removed and homogenized. Mitochondrial fractions were separated and enzyme activities were measured in each sample. H9c2 cells were pretreated with high levels of glucose (35 mM), and then, incubated with PSO. Finally, cell viability test, reactive oxygen species production and lipid peroxidation were evaluated. Results: Significant reduction in enzymes activity (Superoxide dismutase, Glutathione S-transferase and Paraoxonase 1), compensatory elevation in Glutathione Reductase, Glutathione Peroxidase and Catalase activity followed by reduction after one week and significant elevation in Oxidative Stress Index (OSI) were observed in diabetic group. PSO treatment resulted in a significant increase in enzymes activity and decreased OSI values compared to diabetic group in both tissue and mitochondrial fractions. PSO remarkably decreased glucose-induced toxicity, ROS level and lipid peroxidation in H9c2 cells. Conclusion: Results suggested that PSO has a protective effect against diabetes-induced alterations in oxidant/antioxidant balance in tissues, mitochondrial and H9c2 cell line. | ||
| کلیدواژهها | ||
| Pomegranate seed oil؛ Diabetes Mellitus؛ Oxidant؛ Antioxidant؛ Oxidative stress | ||
| مراجع | ||
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