Zinc oxide nanoparticle reduced biofilm formation and antigen 43 expressions in uropathogenic Escherichia coli | ||
| Iranian Journal of Basic Medical Sciences | ||
| مقاله 15، دوره 20، شماره 4، تیر 2017، صفحه 451-456 اصل مقاله (1016.09 K) | ||
| شناسه دیجیتال (DOI): 10.22038/ijbms.2017.8589 | ||
| نویسندگان | ||
| Ali Shakerimoghaddam1؛ Ezzat A Ghaemi1؛ Ailar Jamalli* 2 | ||
| 1Department of Microbiology, Faculty of Medical Sciences, Golestan University of Medical Sciences, Gorgan, Iran | ||
| 2Laboratory Sciences Research Center, Golestan University of Medical Sciences, Gorgan, Iran | ||
| چکیده | ||
| Objective(s): This study aimed to investigate the effect of zinc oxide nanoparticles (ZnO-np) on biofilm formation and expression of the flu gene in uropathogenic Escherichia coli (UPEC) strains. Materials and Methods: Minimum inhibitory concentration (MIC) of ZnO-np was determined by agar dilution method. The effect of MIC and sub-MIC concentrations of ZnO-np on biofilm formation were determined by microtiter plate assay. The expression level of the flu gene was assessed by Real-Time PCR assay. Results: MIC and sub-MIC ZnO-np concentrations reduced biofilm formation by 50% and 33.4%, respectively. Sub-MIC ZnO-np concentration significantly reduced the flu gene expression in the UPEC isolates (P<0.0001). Conclusion: The sub-MIC concentration of ZnO-np reduces biofilm formation and flu gene expression in UPEC isolates. It is suggested to use nanoparticles for coating medical devices to prevent bacterial colonization. | ||
| کلیدواژهها | ||
| Biofilm؛ Urinary tract infection؛ Uropathogenic Escherichia coli؛ Zinc oxide nanoparticle | ||
| مراجع | ||
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