A multiple-antigen detection assay for tuberculosis diagnosis based on broadly reactive polyclonal antibodies | ||
| Iranian Journal of Basic Medical Sciences | ||
| مقاله 3، دوره 20، شماره 4، تیر 2017، صفحه 360-367 اصل مقاله (1.12 M) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22038/ijbms.2017.8575 | ||
| نویسندگان | ||
| Zhenhua Dai1؛ Zhiqiang Liu1؛ Bingshui Xiu1؛ Xiqin Yang1؛ Ping Zhao2؛ Xuhui Zhang1؛ Cuimi Duan1؛ Haiping Que1؛ Heqiu Zhang1؛ Xiaoyan Feng* 1 | ||
| 1Department of Bio-diagnosis, Beijing Institute of Basic Medical Sciences, 27 Taiping Road, Beijing 100850, China | ||
| 2Chaoyang District Centre for Disease Control and Prevention, 25 Panjiayuan Huaweili, Beijing 100029, China | ||
| چکیده | ||
| Objective(s): Detection of circulating Mycobacterium tuberculosis (M. tuberculosis) antigens is promising in Tuberculosis (TB) diagnosis. However, not a single antigen marker has been found to be widely expressed in all TB patients. This study is aimed to prepare broadly reactive polyclonal antibodies targeting multiple antigen markers (multi-target antibodies) and evaluate their efficacies in TB diagnosis. Materials and Methods: A fusion gene consisting of 38kD, ESAT6, and CFP10 was constructed and overexpressed. The fusion polyprotein was used as an immunogen to elicit production of multi-target antibodies. Their reactivities were tested. Then, the multi-target antibodies and three corresponding antibodies elicited by each single antigen (mono-target antibodies) were evaluated with sandwich ELISA for detecting M. tuberculosis antigens. Their diagnostic efficacies for TB were also compared. Results: The polyprotein successfully elicited production of multi-target antibodies targeting 38kD, ESAT6, and CFP10 as analyzed by Western blotting. When used as coating antibodies, the multi-target antibodies were more efficient in capturing the three antigens than the corresponding mono-target antibodies. By testing clinical serum, the multi-target antibodies demonstrated significantly higher sensitivity for clinical TB diagnosis than all three mono-target antibodies. Conclusion: The multi-target antibodies allowed detecting multiple antigens simultaneously and significantly enhanced TB detection compared to routine mono-target antibodies. Our study may provide a promising strategy for TB diagnosis. | ||
| کلیدواژهها | ||
| Antibodies؛ Antigen؛ Diagnosis؛ Tuberculosis؛ ELISA | ||
| مراجع | ||
|
1. Cheng Y, Xie H, Sule P, Hassounah H, Graviss EA, Kong Y, et al. Fluorogenic Probes with Substitutions at the 2 and 7 Positions of Cephalosporin are Highly BlaC-Specific for Rapid Mycobacterium Tuberculosis Detection. Angew Chem Int Ed Engl2014; 53:9360-9364.
2. Raja A, Ranganathan UD, Bethunaickan R. Improved diagnosis of pulmonary tuberculosis by detection of antibodies against multiple Mycobacterium tuberculosis antigens. Diagn Microbiol Infect Dis2008; 60:361-368.
3. Zhang X, Su Z, Hu C, Yu J, Gao Q, Wang H. Generation of Mycobacterium tuberculosis-specific recombinant antigens and evaluation of the clinical value of antibody detection for serological diagnosis of pulmonary tuberculosis. Int J Mol Med2013, 31:751-757.
4. Ireton GC, Greenwald R, Liang H, Esfandiari J, Lyashchenko KP, Reed SG. Identification of Mycobacterium tuberculosis antigens of high serodiagnostic value. Clin Vaccine Immunol2010, 17:1539-1547.
5. Zhang MM, Zhao JW, Sun ZQ, Liu J, Guo XK, Liu WD et al. Identification of RD5-encoded Mycobacterium tuberculosis proteins as B-cell antigens used for serodiagnosis of tuberculosis. Clin Dev Immunol2012; 2012:738043.
6. Tanimura T, Jaramillo E, Weil D, Raviglione M, Lonnroth K. Financial burden for tuberculosis patients in low- and middle-income countries: a systematic review. Eur Respir J2014; 43:1763-1775.
7. Brodwall K. [Diagnosis of tuberculosis in low and middle income countries]. Tidsskr Nor Laegeforen2012; 132:521.
8. Qi Y, Cui L, Ge Y, Shi Z, Zhao K, Guo X et al. Altered serum microRNAs as biomarkers for the early diagnosis of pulmonary tuberculosis infection. BMC Infect Dis2012; 12:384.
9. Adelman MW, Kurbatova E, Wang YF, Leonard MK, White N, McFarland DA et al. Cost analysis of a nucleic acid amplification test in the diagnosis of pulmonary tuberculosis at an urban hospital with a high prevalence of TB/HIV. PLoS One 2014, 9:e100649.
10. Belard S, Andronikou S, Pillay T, Grobusch MP, Zar HJ. New imaging approaches for improving diagnosis of childhood tuberculosis. S Afr Med J2014; 104:181-182.
11. Trajman A, da Silva Santos Kleiz de Oliveira EF, Bastos ML, Belo Neto E, Silva EM, da Silva Lourenco MC et al. Accuracy of polimerase chain reaction for the diagnosis of pleural tuberculosis. Respir Med2014; 108:918-923.
12. Baumann R, Kaempfer S, Chegou NN, Oehlmann W, Loxton AG, Kaufmann SH et al. Serologic diagnosis of tuberculosis by combining Ig classes against selected mycobacterial targets. J Infect2014; 69:581-589.
13. Harinath BC, Kumar S, Roy SS, Hirudkar S, Upadhye V, Shende N. A cocktail of affinity-purified antibodies reactive with diagnostically useful mycobacterial antigens ES-31, ES-43, and EST-6 for detecting the presence of Mycobacterium tuberculosis. Diagn Microbiol Infect Dis2006; 55:65-68.
14. Liu Z, Qie S, Li L, Xiu B, Yang X, Dai Z et al. Identification of Novel RD1 Antigens and Their combinations for diagnosis of sputum smear-/culture+ TB patients. Biomed Res Int2016; 2016:7486425.
15. Legesse M, Ameni G, Medhin G, Mamo G, Franken KL, Ottenhoff TH et al. IgA response to ESAT-6/CFP-10 and Rv2031 antigens varies in patients with culture-confirmed pulmonary tuberculosis, healthy Mycobacterium tuberculosis-infected and non-infected individuals in a tuberculosis endemic setting, Ethiopia. Scand J Immunol2013; 78:266-274.
16. Majumdar A, Kamble PD, Harinath BC. Detection of circulating free and immune-complexed antigen in pulmonary tuberculosis using cocktail of antibodies to mycobacterium tuberculosis excretory secretory antigens by peroxidase enzyme immunoassay. Indian J Tuberc2010; 57:67-74.
17. van Pinxteren LA, Ravn P, Agger EM, Pollock J, Andersen P. Diagnosis of tuberculosis based on the two specific antigens ESAT-6 and CFP10. Clin Diagn Lab Immunol2000; 7:155-160.
18. Feng X, Yang X, Xiu B, Qie S, Dai Z, Chen K et al. IgG, IgM and IgA antibodies against the novel polyprotein in active tuberculosis. BMC Infect Dis2014; 14:336.
19. Feng X, Xiu B, Chen K, Yang X, Zhang H, Yue J et al. Enhanced serodiagnostic utility of novel Mycobacterium tuberculosis polyproteins. J Infect2013; 66:366-375.
20. Cho OH, Park KH, Park SJ, Kim SM, Park SY, Moon SM et al. Rapid diagnosis of tuberculous peritonitis by T cell-based assays on peripheral blood and peritoneal fluid mononuclear cells. J Infect2011; 62:462-471.
21. Yan L, Xiao H, Zhang Q. Systematic review: Comparison of Xpert MTB/RIF, LAMP and SAT methods for the diagnosis of pulmonary tuberculosis. Tuberculosis (Edinb)2016; 96:75-86.
22. Sehgal IS, Dhooria S, Aggarwal AN, Behera D, Agarwal R. Diagnostic Performance of Xpert MTB/RIF in Tuberculous Pleural Effusion: Systematic Review and Meta-analysis. J Clin Microbiol2016; 54:1133-1136.
23. Lawn SD, Zumla AI. Tuberculosis. Lancet2011; 378:57-72.
24. Anderson BL, Welch RJ, Litwin CM. Assessment of three commercially available serologic assays for detection of antibodies to Mycobacterium tuberculosis and identification of active tuberculosis. Clin Vaccine Immunol2008; 15:1644-1649.
25. Kalra M, Khuller GK, Grover A, Behera D, Wanchu A, Verma I. Utility of a combination of RD1 and RD2 antigens as a diagnostic marker for tuberculosis. Diagn Microbiol Infect Dis2010; 66:153-161.
26. Flores LL, Steingart KR, Dendukuri N, Schiller I, Minion J, Pai M et al. Systematic review and meta-analysis of antigen detection tests for the diagnosis of tuberculosis. Clin Vaccine Immunol2011; 18:1616-1627.
27. Demkow U, Filewska M, Michalowska-Mitczuk D, Kus J, Jagodzinski J, Zielonka T et al. Heterogeneity of antibody response to myobacterial antigens in different clinical manifestations of pulmonary tuberculosis. J Physiol Pharmacol2007; 58:117-127.
28. Deng S, Yuan T, Xia J, Huang H, Cheng X, Chen M. Clinical utility of a combination of lipoarabinomannan, 38-kDa, and 16-kDa antigens as a diagnosis tool for tuberculosis. Diagn Microbiol Infect Dis2011; 71:46-50. 29. Bekmurzayeva A, Sypabekova M, Kanayeva D. Tuberculosis diagnosis using immunodominant, secreted antigens of Mycobacterium tuberculosis. Tuberculosis (Edinb)2013; 93:381-388.
30. Vordermeier HM, Whelan A, Cockle PJ, Farrant L, Palmer N, Hewinson RG. Use of synthetic peptides derived from the antigens ESAT-6 and CFP-10 for differential diagnosis of bovine tuberculosis in cattle. Clin Diagn Lab Immunol2001; 8:571-578.
31. Parkash O, Singh BP, Pai M. Regions of differences encoded antigens as targets for immunodiagnosis of tuberculosis in humans. Scand J Immunol2009; 70:345-357.
32. Kanekiyo M, Wei CJ, Yassine HM, McTamney PM, Boyington JC, Whittle JR et al. Self-assembling influenza nanoparticle vaccines elicit broadly neutralizing H1N1 antibodies. Nature2013; 499:102-106. | ||
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