سامانه مدیریت نشریات علمی دانشگاه علوم پزشکی مشهد

Dehghan Esmatabadi, Mohammad Javad, Farhangi, Baharak, Montazeri, Maryam, Monfared, Hamideh, Nakhaei Sistani, Roohollah, Sadeghizadeh, Majid. (1396). Up-regulation of miR-21 decreases chemotherapeutic effect of dendrosomal curcumin in breast cancer cells. , 20(4), 350-359. doi: 10.22038/ijbms.2017.8574
Mohammad Javad Dehghan Esmatabadi; Baharak Farhangi; Maryam Montazeri; Hamideh Monfared; Roohollah Nakhaei Sistani; Majid Sadeghizadeh. "Up-regulation of miR-21 decreases chemotherapeutic effect of dendrosomal curcumin in breast cancer cells". , 20, 4, 1396, 350-359. doi: 10.22038/ijbms.2017.8574
Dehghan Esmatabadi, Mohammad Javad, Farhangi, Baharak, Montazeri, Maryam, Monfared, Hamideh, Nakhaei Sistani, Roohollah, Sadeghizadeh, Majid. (1396). 'Up-regulation of miR-21 decreases chemotherapeutic effect of dendrosomal curcumin in breast cancer cells', , 20(4), pp. 350-359. doi: 10.22038/ijbms.2017.8574
Dehghan Esmatabadi, Mohammad Javad, Farhangi, Baharak, Montazeri, Maryam, Monfared, Hamideh, Nakhaei Sistani, Roohollah, Sadeghizadeh, Majid. Up-regulation of miR-21 decreases chemotherapeutic effect of dendrosomal curcumin in breast cancer cells. , 1396; 20(4): 350-359. doi: 10.22038/ijbms.2017.8574

Up-regulation of miR-21 decreases chemotherapeutic effect of dendrosomal curcumin in breast cancer cells

Iranian Journal of Basic Medical Sciences
مقاله 2، دوره 20، شماره 4، تیر 2017، صفحه 350-359    اصل مقاله (1.24 M)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2017.8574
نویسندگان
Mohammad Javad Dehghan Esmatabadi1؛ Baharak Farhangi1؛ Maryam Montazeri2؛ Hamideh Monfared1؛ Roohollah Nakhaei Sistani3؛ Majid Sadeghizadeh* 1
1Department of Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran
2Department of Medical Biotechnology, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran
3Department of Cellular Biotechnology, Faculty of Chemistry, Kashan University, Kashan, Iran
چکیده
Objective(s): Despite the good results of anticancer activities by curcumin, there are some hurdles that limit the use of curcumin as an anticancer agent. Many methods were examined to overcome this defect like the use of the dendrosomal curcumin (DNC). There is increasing evidence that miRNAs play important roles in biological processes. In this study, we focus on the roles of microRNA-21 in the anti-cancer effects of DNC in breast cancer.
Materials and Methods: Also, we have used different methods such as MTT, apoptosis, cell cycle analysis, transwell migration assay and RT-PCR to find out more.
Results: We observed that miR-21 decreased apoptotic cells in both cells (from 6.35% to 0.34 % and from 7.72% to 1.32% orderly) and DNC increased it. As well as, our findings indicated that cell migration capacity was increased by miR-21 over expression and was decreased by DNC. The combination of miR-21 vector transfection and DNC treatment showed lower percentage of apoptotic cells or a higher level of penetration through the membrane compared with DNC treatment alone. Furthermore, DNC induced a marked increase in the number of cells in sub G1/G1 phase and a decrease in G2/M phase of the cell cycle in both; but, we observed reverse results compared it, after transfection with miR-21 vector.
Conclusion: We observed that miR-21 suppress many aspects of anti-cancer effects of DNC in breast cancer cells, it seems that co-treatment with DNC and mir-21 down-regulation may provide a clinically useful tool for drug-resistance breast cancer cells.
کلیدواژه‌ها
Breast Cancer؛ Cell cycle؛ Cell Proliferation؛ Curcumin؛ Dendrosomal curcumin؛ MicroRNA-21
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