Tabasi, Akramsadat, Ghafari, Soraya, Mehdizadeh, Mehdi, Asadi Shekari, Majid, Golalipour, Mohammad Jafar. (1395). Gestational diabetes influences retinal Muller cells in rat's offspring. , 20(2), 216-221. doi: 10.22038/ijbms.2017.8251
Akramsadat Tabasi; Soraya Ghafari; Mehdi Mehdizadeh; Majid Asadi Shekari; Mohammad Jafar Golalipour. "Gestational diabetes influences retinal Muller cells in rat's offspring". , 20, 2, 1395, 216-221. doi: 10.22038/ijbms.2017.8251
Tabasi, Akramsadat, Ghafari, Soraya, Mehdizadeh, Mehdi, Asadi Shekari, Majid, Golalipour, Mohammad Jafar. (1395). 'Gestational diabetes influences retinal Muller cells in rat's offspring', , 20(2), pp. 216-221. doi: 10.22038/ijbms.2017.8251
Tabasi, Akramsadat, Ghafari, Soraya, Mehdizadeh, Mehdi, Asadi Shekari, Majid, Golalipour, Mohammad Jafar. Gestational diabetes influences retinal Muller cells in rat's offspring. , 1395; 20(2): 216-221. doi: 10.22038/ijbms.2017.8251

Gestational diabetes influences retinal Muller cells in rat's offspring

Iranian Journal of Basic Medical Sciences
مقاله 16، دوره 20، شماره 2، 2017، صفحه 216-221    اصل مقاله (1.09 M)
نوع مقاله: Short Communication
شناسه دیجیتال (DOI): 10.22038/ijbms.2017.8251
نویسندگان
Akramsadat Tabasi1؛ Soraya Ghafari2؛ Mehdi Mehdizadeh3؛ Majid Asadi Shekari4؛ Mohammad Jafar Golalipour* 2
1Department of Anatomical Sciences, Golestan University of Medical Sciences, Gorgan, Iran
2Gorgan Congenital Malformations Research Center, Department of Anatomical Sciences, Golestan University of Medical Sciences, Gorgan, Iran
3Department of Anatomical Sciences, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
4Neuroscience Research Center, Neuropharmacology Institute. Kerman University of Medical Sciences, Kerman, Iran
چکیده
Objective(s): The Muller cell is the principal glial cell of the vertebrate retina. The expression of Glial fibrillary acidic protein (GFAP) in the Muller cells was used as a cellular marker for retinal damage. This study was done to evaluate the effect of gestational diabetes on retinal Muller cells in rat's offspring.
Materials and Methods: In this experimental study, 12 Wistar rat dams were randomly allocated in control and diabetic groups. Gestational diabetes was induced by 40 mg/kg/body weight of streptozotocin at the first day of gestation, intraperitoneally. Dams in control group received an equivalent volume normal saline. Eye of six offspring of each group were removed at postnatal day 28 (P28). The histopathological changes in retina were examined through H&E staining and ultrastructure transmission electron microscopy (TEM). The expression of GFAP was examined using Immunohisto-chemical staining of GFAP in Muller cells. Photographs of retina were taken using Olympus BX51 microscope and a digital camera DP12 and EM LEO906; Zeiss, Germany.
Results: In the control rat's offspring, GFAP expression was not significant in Muller cells. According to the optical microscope images, GFAP expression was observed in the processes of the Muller cell in the inner plexiform layer of retina in offspring of diabetic mothers. In TEM technique, nuclear fragmentation and apoptotic bodies were observed in Muller cell of diabetic offspring.
Conclusion: This study showed that the uncontrolled gestational diabetes can increase GFAP expression in Muller cells and retinal thickness of retinal layer in rat offspring's, therefore uncontrolled gestational can damage the Muller cells.
کلیدواژه‌ها
Gestational diabetes؛ GFAP؛ Mellitus؛ Muller cell؛ Retina
مراجع
آمار
تعداد مشاهده مقاله: 1,881
تعداد دریافت فایل اصل مقاله: 1,392
دانشگاه علوم پزشکی مشهد
آمار
تعداد نشریات29
تعداد شماره‌ها2,324
تعداد مقالات24,868
تعداد مشاهده مقاله82,849,766
تعداد دریافت فایل اصل مقاله45,911,093
اخبار نشریات