Anti-nociceptive and anti-inflammatory effects of the methanolic extract of Opuntia humifusa stem | ||
| Avicenna Journal of Phytomedicine | ||
| مقاله 8، دوره 7، شماره 4، مهر و آبان 2017، صفحه 366-375 اصل مقاله (569.53 K) | ||
| نوع مقاله: Original Research Article | ||
| شناسه دیجیتال (DOI): 10.22038/ajp.2017.19155.1740 | ||
| نویسندگان | ||
| Bhesh Raj Sharma1؛ Chul Min Park1؛ Jong Won Choi2؛ Dong Young Rhyu* 1 | ||
| 1Department of Oriental Medicine Resources Mokpo National University, Muan-gun, Jeonnam 58554, Republic of Korea | ||
| 2College of Pharmacy, Kyungsung University, Busan 48434, Korea | ||
| چکیده | ||
| Objective: Opuntia humifusa (O. humifusa) Raf. has been used for the prevention and treatment of rheumatoid arthritis, inflammation, and cancer. Our study was designed to unveil the anti-nociceptive and anti-inflammatory effects of the methanolic extract of O. humifusa Raf stem (OHS). Material and Methods: The anti-nociceptive effect was measured by hot plate, acetic acid-induced writhing, and tail flick assays in mice and rats. Moreover, the anti-inflammatory effect was measured by vascular permeability and carrageenan and serotonin-induced paw edema tests in rats. Furthermore, anti-inflammatory effect was also measured using macrophage-like LPS-induced RAW 264.7 cells. Results: OHS extract inhibited acetic acid-induced writhing (p<0.0001), and delayed the reaction time of mice to the hot plate-induced thermal stimulation (p<0.0001) and tail flick tests (p<0.05). OHS extract attenuated the carrageenan and serotonin-induced paw edema in rats (p<0.001). Similarly, OHS extract significantly decreased Evans blue concentration in acetic acid induced vascular permeability test (p<0.0001), revealing its strong anti-inflammatory effect. Finally, among four different fractions of OHS extract, n-butanol fraction strongly decreased NO production (p<0.0001) and iNOS expression in LPS-induced RAW 264.7 cells. Conclusion: Our results suggest that the methanolic extract of O. humifusa stem can be used to develop a therapeutic or supportive drug and/or functional food against pain and inflammation related diseases. | ||
| کلیدواژهها | ||
| Opuntia humifusa؛ Anti-nociceptive؛ Anti-inflammatory؛ RAW 264.7 cells | ||
| مراجع | ||
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