Baghani, Ali Asghar, Soleimanpour, Saman, Farsiani, Hadi, Mosavat, Arman, Yousefi, Masoud, Meshkat, Zahra, Rezaee, Seyed Abdolrahim, Amel Jamehdar, Saeid, Akbari Eydgahi, Mohammad Reza, Sadeghian, Hamid, Ghazvini, Kiarash. (1395). CFP10: mFcγ2 as a novel tuberculosis vaccine candidate increases immune response in mouse. , 20(2), 122-130. doi: 10.22038/ijbms.2017.8231
Ali Asghar Baghani; Saman Soleimanpour; Hadi Farsiani; Arman Mosavat; Masoud Yousefi; Zahra Meshkat; Seyed Abdolrahim Rezaee; Saeid Amel Jamehdar; Mohammad Reza Akbari Eydgahi; Hamid Sadeghian; Kiarash Ghazvini. "CFP10: mFcγ2 as a novel tuberculosis vaccine candidate increases immune response in mouse". , 20, 2, 1395, 122-130. doi: 10.22038/ijbms.2017.8231
Baghani, Ali Asghar, Soleimanpour, Saman, Farsiani, Hadi, Mosavat, Arman, Yousefi, Masoud, Meshkat, Zahra, Rezaee, Seyed Abdolrahim, Amel Jamehdar, Saeid, Akbari Eydgahi, Mohammad Reza, Sadeghian, Hamid, Ghazvini, Kiarash. (1395). 'CFP10: mFcγ2 as a novel tuberculosis vaccine candidate increases immune response in mouse', , 20(2), pp. 122-130. doi: 10.22038/ijbms.2017.8231
Baghani, Ali Asghar, Soleimanpour, Saman, Farsiani, Hadi, Mosavat, Arman, Yousefi, Masoud, Meshkat, Zahra, Rezaee, Seyed Abdolrahim, Amel Jamehdar, Saeid, Akbari Eydgahi, Mohammad Reza, Sadeghian, Hamid, Ghazvini, Kiarash. CFP10: mFcγ2 as a novel tuberculosis vaccine candidate increases immune response in mouse. , 1395; 20(2): 122-130. doi: 10.22038/ijbms.2017.8231

CFP10: mFcγ2 as a novel tuberculosis vaccine candidate increases immune response in mouse

Iranian Journal of Basic Medical Sciences
مقاله 3، دوره 20، شماره 2، 2017، صفحه 122-130    اصل مقاله (1.15 M)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2017.8231
نویسندگان
Ali Asghar Baghani1، 2؛ Saman Soleimanpour1، 2؛ Hadi Farsiani1، 2؛ Arman Mosavat3؛ Masoud Yousefi1، 2؛ Zahra Meshkat1، 2؛ Seyed Abdolrahim Rezaee4؛ Saeid Amel Jamehdar1، 2؛ Mohammad Reza Akbari Eydgahi5؛ Hamid Sadeghian6؛ Kiarash Ghazvini* 1، 2
1Antimicrobial Resistance Research Center, Bu-Ali Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran
2Department of Microbiology and Virology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
3HIV/AIDS, HTLV and Viral Hepatitis Research Center, Iranian Academic Center for Education, Culture and Research (ACECR), Mashhad, Iran
4Immunology Research Center, Inflammation and Inflammatory Diseases Division, Medical school, Mashhad University of Medical Sciences, Mashhad, Iran
5Biotechnology Research Center, Semnan University of Medical Sciences, Semnan, Iran
6Organic Chemistry, Department of Laboratory Sciences, Mashhad University of Medical Sciences, Mashhad, Iran
چکیده
Objective(s): Despite treatment with antibiotics and vaccination with BCG, tuberculosis (TB) is still considered as one of the most important public health problems in the world. Therefore, designing and producing a more effective vaccine against TB seems urgently. In this study, immunogenicity of a fusion protein which consisting or comprising CFP-10 from Mycobacterium tuberculosis and the Fc-domain of  mouse IgG2a was evaluated as a novel subunit vaccine candidate against TB.
Materials and Methods: The genetic constructs were cloned in pPICZαA expression vector and recombinant vectors (pPICZαA-CFP-10: Fcγ2a and pPICZαA-CFP-10:His) were transformed into Pichia pastoris. To evaluate the expression of recombinant proteins, SDS-PAGE and immunoblotting were used. The immunogenicity of recombinant proteins, with and without BCG were assessed in BALB/c mice and specific cytokines against recombinant proteins (IFN-γ, IL-12, IL-4, IL-17 and TGF-β) were evaluated.
Results: The levels of IFN-γ and IL-12 in mice that received recombinant proteins was higher than the control groups (BCG and PBS). Thus, both recombinant proteins (CFP-10:Fcγ2a and CFP-10:His) could excite good response in Th1-cells. The Fc-tagged protein had a stronger Th1 response with low levels of IL-4, as compared to CFP-10:His. However, the highest level of Th1 response was observed in groups that were vaccinated with BCG (prime) and then received recombinant protein CFP-10: Fcγ2a (booster).
Conclusion: The results demonstrated that binding mice Fc-domain to CFP-10 protein can increase the immunogenicity of the subunit vaccine. Further studies, might be able to design and produce a new generation of subunit vaccines based on the Fc-fused immunogen.
کلیدواژه‌ها
CFP-10؛ Fcγ2a؛ Immunogenicity؛ Mycobacterium tuberculosis؛ Subunit vaccine
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