CFP10: mFcγ2 as a novel tuberculosis vaccine candidate increases immune response in mouse | ||
| Iranian Journal of Basic Medical Sciences | ||
| مقاله 3، دوره 20، شماره 2، اردیبهشت 2017، صفحه 122-130 اصل مقاله (1.15 M) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22038/ijbms.2017.8231 | ||
| نویسندگان | ||
| Ali Asghar Baghani1، 2؛ Saman Soleimanpour1، 2؛ Hadi Farsiani1، 2؛ Arman Mosavat3؛ Masoud Yousefi1، 2؛ Zahra Meshkat1، 2؛ Seyed Abdolrahim Rezaee4؛ Saeid Amel Jamehdar1، 2؛ Mohammad Reza Akbari Eydgahi5؛ Hamid Sadeghian6؛ Kiarash Ghazvini* 1، 2 | ||
| 1Antimicrobial Resistance Research Center, Bu-Ali Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran | ||
| 2Department of Microbiology and Virology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran | ||
| 3HIV/AIDS, HTLV and Viral Hepatitis Research Center, Iranian Academic Center for Education, Culture and Research (ACECR), Mashhad, Iran | ||
| 4Immunology Research Center, Inflammation and Inflammatory Diseases Division, Medical school, Mashhad University of Medical Sciences, Mashhad, Iran | ||
| 5Biotechnology Research Center, Semnan University of Medical Sciences, Semnan, Iran | ||
| 6Organic Chemistry, Department of Laboratory Sciences, Mashhad University of Medical Sciences, Mashhad, Iran | ||
| چکیده | ||
| Objective(s): Despite treatment with antibiotics and vaccination with BCG, tuberculosis (TB) is still considered as one of the most important public health problems in the world. Therefore, designing and producing a more effective vaccine against TB seems urgently. In this study, immunogenicity of a fusion protein which consisting or comprising CFP-10 from Mycobacterium tuberculosis and the Fc-domain of mouse IgG2a was evaluated as a novel subunit vaccine candidate against TB. Materials and Methods: The genetic constructs were cloned in pPICZαA expression vector and recombinant vectors (pPICZαA-CFP-10: Fcγ2a and pPICZαA-CFP-10:His) were transformed into Pichia pastoris. To evaluate the expression of recombinant proteins, SDS-PAGE and immunoblotting were used. The immunogenicity of recombinant proteins, with and without BCG were assessed in BALB/c mice and specific cytokines against recombinant proteins (IFN-γ, IL-12, IL-4, IL-17 and TGF-β) were evaluated. Results: The levels of IFN-γ and IL-12 in mice that received recombinant proteins was higher than the control groups (BCG and PBS). Thus, both recombinant proteins (CFP-10:Fcγ2a and CFP-10:His) could excite good response in Th1-cells. The Fc-tagged protein had a stronger Th1 response with low levels of IL-4, as compared to CFP-10:His. However, the highest level of Th1 response was observed in groups that were vaccinated with BCG (prime) and then received recombinant protein CFP-10: Fcγ2a (booster). Conclusion: The results demonstrated that binding mice Fc-domain to CFP-10 protein can increase the immunogenicity of the subunit vaccine. Further studies, might be able to design and produce a new generation of subunit vaccines based on the Fc-fused immunogen. | ||
| کلیدواژهها | ||
| CFP-10؛ Fcγ2a؛ Immunogenicity؛ Mycobacterium tuberculosis؛ Subunit vaccine | ||
| مراجع | ||
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