In vitro assesment of anti-inflammatory activities of coumarin and Indonesian cassia extract in RAW264.7 murine macrophage cell line | ||
| Iranian Journal of Basic Medical Sciences | ||
| مقاله 15، دوره 20، شماره 1، فروردین 2017، صفحه 99-106 اصل مقاله (1.04 M) | ||
| نوع مقاله: Short Communication | ||
| شناسه دیجیتال (DOI): 10.22038/ijbms.2017.8102 | ||
| نویسندگان | ||
| Ni Made Dwi Sandhiutami1؛ Moordiani Moordiani1؛ Dian Ratih Laksmitawati1؛ Nurul Fauziah2؛ Maesaroh Maesaroh2؛ Wahyu Widowati* 3 | ||
| 1Faculty of Pharmacy, University of Pancasila, Jl. Srenseng Sawah, Jagakarsa, Jakarta 12640, Indonesia | ||
| 2Bimolecular and Biomedical Research Center, Aretha Medika Utama,Jl. Babakan Jeruk 2 no 9, Bandung 40163, Indonesia | ||
| 3Medical Research Center, Faculty of Medicine, Maranatha Christian University, Jl. Prof. drg. Suria Sumantri no 65 Bandung 40164, Indonesia | ||
| چکیده | ||
| Objective(s): Inflammation is an immune response toward injuries. Although inflammation is healing response, but in some condition it will lead to chronic disease such as rheumatoid arthritis, inflammatory bowel disease, atherosclerosis, Alzheimer’s and various cancer. Indonesian cassia (Cinnamomum burmanni C. Nees & T. Ness) known to contain coumarin, is widely used for alternative medicine especially as an antiinflammator.This study was conducted to determine the anti-inflammatory properties of coumarin and Indonesian cassia extract (ICE) in LPS-induced RAW264.7 cell line. Materials and Methods: The cytotoxic assay of coumarin and ICE against RAW264.7 cells was conducted using MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium). The anti-inflammatory potential was determined using LPS-induced RAW 267.4 macrophages cells to measure inhibitory activity of both compounds on production of nitric oxide (NO), prostaglandin E2 (PGE2), and also cytokines such as interleukin-6 (IL-6), interleukin-1β (IL-1β) and TNF-α. Results: Coumarin 10 µM and ICE 10 µg/ml were nontoxic to the RAW264.7 cells. Both of coumarin and ICE were capable to reduce the PGE2, TNF-α, NO, IL-6, and IL-β level in LPS-induced RAW264.7 cells. Coumarin had higher activity to decrease PGE2 and TNF-α, whilst ICE had higher activity to inhibit NO, IL-6, and IL-β levels. Conclusion: Coumarin and ICE possess anti-inflammatory properties through inhibition of PGE2 and NO along with pro-inflammatory cytokines TNF-α, IL-6, IL-1β production. | ||
| کلیدواژهها | ||
| Coumarin؛ Cytokines؛ Indonesian cassia extract؛ Inflammation؛ RAW264.7 | ||
| مراجع | ||
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