Antioxidant and antiapoptotic effects of erdosteine in a rat model of ovarian ischemia-reperfusion injury | ||
| Iranian Journal of Basic Medical Sciences | ||
| مقاله 9، دوره 20، شماره 1، فروردین 2017، صفحه 53-58 اصل مقاله (1.4 M) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22038/ijbms.2017.8093 | ||
| نویسندگان | ||
| Vedat Ugurel1؛ Ahmet Cagatay Cicek2؛ Mustafa Cemek3؛ Selim Demirtas2؛ A Tuba Kocaman3؛ Turan Karaca* 2 | ||
| 1University of Trakya, Faculty of Medicine, Department of Obstetrics and Gynecology, 22030, Balkan Campus, Edirne, Turkey | ||
| 2University of Trakya, Faculty of Medicine, Department of Histology and Embryology, 22030, Balkan Campus, Edirne, Turkey | ||
| 3Yildiz Technical University, Biochemistry Division, Department of Bioengineering, Faculty of Chemical and Metallurgical Engineering, Istanbul, Turkey | ||
| چکیده | ||
| Objective(s): To evaluate the protective effect of erdosteine, an antiapoptotic and antioxidant agent, on torsion–detorsion evoked histopathological changes in experimental ovarian ischemia-reperfusion (IR) injury. Materials and Methods: Eighteen female Wistar albino rats were used in control, IR, and IR+Edosteine (IR-E) groups, (n=6 in each). The IR-E group received the erdosteine for seven days before the induction of torsion/retorsion, (10 mg/kg/days). The IR and IR-E groups were exposed to right unilateral adnexal torsion for 3 hr. Three hours later, re-laparotomy was performed, and the right ovaries were surgically excised. Oxidant and antioxidants levels were determined in serum. The ovarian tissue samples were received and fixed with 10% neutral buffered formalin. The sections were stained with H&E, anti-PCNA, and TUNEL. Results: The IR group were showed severe acute inflammation, polynuclear leukocytes and macrophages, stromal oedema and haemorrhage. Treatment with erdosteine in rats significantly retained degenerative changes in the ovaryPCNA (+) cell numbers were significantly decreased in the IR and IR-E groups unlike the control group. However, its numbers were significantly increased in the IR-E group unlike the IR group. TUNEL (+) cell numbers were significantly increased in the IR group unlike the control and the IR-E groups. In erdosteine treated group, TUNEL (+) cells were detected significantly less than the IR group (P<0.05). Conclusion: In conclusion, erdosteine maybe a protective agent for ovarian damage and decreasing lipid peroxidation products and leukocytes aggregation after adnexal torsion in animals. | ||
| کلیدواژهها | ||
| Antioxidant؛ Erdosteine؛ Ischemia-reperfusion Oxidant؛ Ovary؛ Rat | ||
| مراجع | ||
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