MicroRNA-297a regulates vascular calcification by targeting fibroblast growth factor 23 | ||
| Iranian Journal of Basic Medical Sciences | ||
| مقاله 10، دوره 19، شماره 12، اسفند 2016، صفحه 1331-1336 اصل مقاله (1.1 M) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22038/ijbms.2016.7920 | ||
| نویسندگان | ||
| Shouhua Zheng؛ Shuijun Zhang* ؛ Yan Song؛ Wenzhi Guo؛ Wenlong Zhai؛ Xinguang Qiu؛ Jianhua Li | ||
| Department of General Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, 450052 China | ||
| چکیده | ||
| Objective(s): Vascular calcification is one the major characteristics in patients with various types of chronic inflammatory disorders. MiRNAs have been shown to be involved in many normal biological functions as well as diseases; however, their role in vascular calcification has not received much attention. Materials and Methods: In the current study, we built a vascular calcification rat model using vitamin D3 plus nicotine and analyzed miRNA expression profile by miRNA chip assay. Potential target of one selected miRNA with sharpest variation in expression were predicted by both PicTar and TargetScan. The impact of the selected miRNA on the expression of the potential target on both mRNA and protein levels were measured by RT-PCR and Western blot, respectively. Results: Our results identified 16 dysregulated miRNAs, among which miR-297a showed the sharpest variation. Further analysis focusing on miR-297a revealed that fibroblast growth factor 23 (FGF23) was a potential target of miR297a. Measurement of FGF23 and its regulator Klotho on both mRNA and protein levels demonstrated that FGF23 was significantly increased while Klotho was decreased in rats with vascular calcification. Conclusion: Our results indicated that FGF23 was target of miR-297a and decreased miR-297a in vascular calcification lead to the increase of FGF23, which together with Klotho might enhance vascular calcification. The findings of this study could provide valuable information for the understanding of mechanisms underlying miR-dependent vascular calcification as well as potential treatment target for the disease. | ||
| کلیدواژهها | ||
| Fibroblast growth factor 23؛ MicroRNA-297a؛ Chronic inflammatory disorder؛ Vascular calcification؛ Klotho | ||
| مراجع | ||
|
1. Demer LL, Tintut Y. Vascular calcification: pathobiology of a multifaceted disease. Circulation 2008; 117:2938-2948. 2. Zhu D, Mackenzie NC, Farquharson C, MacRae VE. Mechanisms and clinical consequences of vascular calcification. Front Endocrinol 2012; 6;3:95. 3. Komori T. Regulation of bone development and extracellular matrix protein genes by RUNX2. Cell Tissue Res 2010; 339:189-195. 4. Thompson B, Towler DA. Arterial calcification and bone physiology: role of the bone–vascular axis. Nat Rev Endocrinol 2012; 8:529-543. 5. Danilevicius C, Lopes J, Pereira R. Bone metabolism and vascular calcification. Braz J Med Biol Res 2007; 40:435-442. 6. Ambros V. The functions of animal microRNAs. Nature 2004; 431:350-355. 7. Bartel DP. MicroRNAs: target recognition and regulatory functions. Cell 2009; 136:215-233. 8. Small EM, Olson EN. Pervasive roles of microRNAs in cardiovascular biology. Nature 2011; 469:336-342. 9. Goretti E, Vausort M, Wagner DR, Devaux Y. Association between circulating microRNAs, cardiovascular risk factors and outcome in patients with acute myocardial infarction. Int J Cardiol 2013; 168:4548-4550. 10. Zimmerman AL, Wu S. MicroRNAs, cancer and cancer stem cells. Cancer Lett 2011; 300:10-19. 11. Esquela-Kerscher A, Slack FJ. Oncomirs—microRNAs with a role in cancer. Nat Rev Cancer 2006; 6:259-269. 12. Pauley KM, Cha S, Chan EK. MicroRNA in autoimmunity and autoimmune diseases. J Autoimmunity 2009; 32:189-194. 13.Dai R, Ahmed SA. MicroRNA, a new paradigm for understanding immunoregulation, inflammation, and autoimmune diseases. Transl Res 2011; 157:163-179. 14. Goettsch C, Rauner M, Pacyna N, Hempel U, Bornstein SR, Hofbauer LC. miR-125b regulates calcification of vascular smooth muscle cells. Am J Pathol 2011; 179:1594-1600. 15. Cui RR, Li SJ, Liu LJ, Yi L, Liang QH, Zhu X, et al. MicroRNA-204 regulates vascular smooth muscle cell calcification in vitro and in vivo. Cardiovasc Res 2012: 96):320-329. 16. Niederhoffer N, Bobryshev YV, Lartaud-Idjouadiene I, Giummelly P, Atkinson J. Aortic calcification produced by vitamin D3 plus nicotine. J Vasc Res 1997; 34:386-398. 17. Zhang J, Chang JR, Duan XH, Yu YR, Zhang BH. Thyroid hormone attenuates vascular calcification induced by vitamin D3 plus nicotine in rats. Calcif Tissue Int 2015; 96:80-87. 18., Murer H, Hernando N, Forster I, Biber J. Regulation of Na/Pi transporter in the proximal tubule. Ann Rev Physiol 2003; 65:531-542. 19. Urakawa I, Yamazaki Y, Shimada T, Iijima K, Hasegawa H, Okawa K, et al. Klotho converts canonical FGF receptor into a specific receptor for FGF23. Nature 2006; 444:770-774. 20. Hunsberger JG, Fessler EB, Wang Z, Elkahloun AG, Chuang DM. Post-insult valproic acid-regulated microRNAs: potential targets for cerebral ischemia. Am J Transl Res 2012; 4:316-332. 21. Han H, Peng J, Hong Y, Zhang M, Han Y, Fu Z, et al. Comparison of the differential expression miRNAs in Wistar rats before and 10 days after S. japonicum infection. Parasit Vectors 2013; 6:120. 22. Izzotti A, Balansky R, D’Agostini F, Longobardi M, Cartiglia C, La Maestra S, et al. Relationships between pulmonary micro-RNA and proteome profiles, systemic cytogenetic damage and lung tumors in cigarette smoke-exposed mice treated with chemopreventive agents. Carcinogenesis 2013; 34:2322-2329. 23. Fukumoto S. Vascular calcification-pathological mechanism and clinical application-. regulation of mineral metabolism and mineralization by FGF23. Clin Calcium 2015; 25:687-691. 24. Razzaque MS. The FGF23–Klotho axis: endocrine regulation of phosphate homeostasis. Nat Rev Endocrinol 2009; 5:611-619. 25. Hu MC, Shi M, Zhang J, Quiñones H, Griffith C, Kuroo M, et al. Klotho deficiency causes vascular calcification in chronic kidney disease. J Am Soc Nephrol 2011; 22:124-136. 26. Lindberg K, Olauson H, Amin R, Ponnusamy A, Goetz R, Taylor RF, et al. Arterial klotho expression and FGF23 effects on vascular calcification and function. PloS One 2013; 8:e60658. 27. Cui W, Li Q, Feng L, Ding W. MiR-126-3p regulates progesterone receptors and involves development and lactation of mouse mammary gland. Mol Cell Biochem 2011; 355:17-25. 28. Cheng Y, Ji R, Yue J, Yang J, Liu X, Chen H, et al. MicroRNAs are aberrantly expressed in hypertrophic heart: do they play a role in cardiac hypertrophy? Am J Pathol 2007; 170:1831-1840. 29. Zhu S, Pan W, Song X, Liu Y, Shao X, Tang Y, et al. The microRNA miR-23b suppresses IL-17-associated autoimmune inflammation by targeting TAB2, TAB3 and IKK-[alpha]. Nat Med 2012; 18:1077-1086. 30. Zhang H, Hao Y, Yang J, Zhou Y, Li J, Yin S, et al. Genome-wide functional screening of miR-23b as a pleiotropic modulator suppressing cancer metastasis. Nat Commun 2011; 2:554. 31. Banerjee S, Cui H, Xie N, Tan Z, Yang S, Icyuz M, et al. miR-125a-5p regulates differential activation of macrophages and inflammation. J Biol Chem 2013; 32. Manfe V, Biskup E, Willumsgaard A, Skov AG, Palmieri D, Gasparini P, et al. cMyc/miR-125b-5p signalling determines sensitivity to bortezomib in preclinical model of cutaneous T-cell lymphomas. PloS One 2013; 8:e59390. | ||
|
آمار تعداد مشاهده مقاله: 1,851 تعداد دریافت فایل اصل مقاله: 1,546 |
||