G-CSF for mobilizing transplanted bone marrow stem cells in rat model of Parkinson's disease | ||
| Iranian Journal of Basic Medical Sciences | ||
| مقاله 8، دوره 19، شماره 12، اسفند 2016، صفحه 1318-1324 اصل مقاله (951.1 K) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22038/ijbms.2016.7918 | ||
| نویسندگان | ||
| Manouchehr Safari1؛ Behnaz Jafari* 1؛ Sam Zarbakhsh1؛ Hamidreza Sameni1؛ Abbas Ali Vafaei2؛ Nasrin Khan Mohammadi1؛ Laya Ghahari3 | ||
| 1Research Center of Nervous System Stem Cells, Department of Anatomy, Semnan University of Medical Sciences, Semnan, Iran | ||
| 2Department of Physiology, Semnan University of Medical Sciences, Semnan, Iran | ||
| 3Department of Anatomy, AJA University of Medical Sciences, Tehran, Iran | ||
| چکیده | ||
| Objective(s): Granulocyte-colony stimulating factor (G-CSF) is used in clinical practice for the treatment of neutropenia and to stimulate generation of hematopoietic stem cells in bone marrow donors. In the present study, the ability of G-CSF in mobilizing exogenous bone marrow stem cells (BMSCs) from peripheral blood into the brain was tested. We for the first time injected a small amount of BMSCs through the tail vein. Materials and Methods: We choose 25 male Wistar rats (200–250 g) were lesioned by 6-OHDA injected into the left substantia nigra, pars compacta (SNpc). G-CSF (70 µg/kg/day) was given from the 7th day after lesion for five days. The BMSCs (2×105) were injected through the dorsal tail vein on the 7th day after lesion. Results:The number of rotations was significantly lower in the stem cell therapy group than in the control group. In the third test in the received G-CSF and G-CSF+stem cells groups, animals displayed significant behavioral recovery compared with the control group (P<0.05). There was a significant difference in the average of dopaminergic neurons in SNpc between the control group and G-CSF and G-CS+stem cells groups. We didn't detect any labeling stem cells in SNpc. Conclusion: G-CSF can't mobilize low amounts of exogenous BMSCs from the blood stream to injured SNpc. But G-CSF (70 µg/kg) is more neuroprotective than BMSCs (2×105 number of BMSCs). Results of our study suggest that G-CSF alone is more neuroprotective than BMSCs. | ||
| کلیدواژهها | ||
| Bone marrow stem cells؛ Dopaminergic neurons؛ Granulocyte colony stimulating factor؛ Parkinson | ||
| مراجع | ||
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