Survival, proliferation, and migration of human meningioma stem-like cells in a nanopeptide scaffold | ||
| Iranian Journal of Basic Medical Sciences | ||
| مقاله 2، دوره 19، شماره 12، اسفند 2016، صفحه 1271-1278 اصل مقاله (1.55 M) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22038/ijbms.2016.7907 | ||
| نویسندگان | ||
| Sajad Sahab Negah1، 2؛ Hadi Aligholi3؛ Zabihollah Khaksar1؛ Hadi Kazemi4؛ Sayed Mostafa Modarres Mousavi2؛ Maryam Safahani2، 5؛ Parastoo Barati Dowom2؛ Ali Gorji* 2، 6، 7، 8 | ||
| 1Histology and Embryology group, Basic Science Department, Faculty of Veterinary Medicine, Shiraz University, Shiraz, Iran | ||
| 2Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran | ||
| 3Department of Neuroscience, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran | ||
| 4Pediatric Department, Medical Faculty, Shahed University, Tehran, Iran | ||
| 5Department of Community Nutrition, School of Nutritional Sciences and Dietetics, Tehran University of Medical Sciences, Tehran, Iran | ||
| 6Epilepsy Research Center, Westfälische Wilhelms-Universität Münster, Germany | ||
| 7Department of Neurology and Department of Neurosurgery, Westfälische Wilhelms-Universität Münster, Germany | ||
| 8Department of Neuroscience, Mashhad University of Medical Sciences, Mashhad, Iran | ||
| چکیده | ||
| Objective(s): In order to grow cells in a three-dimensional (3D) microenvironment, self-assembling peptides, such as PuraMatrix, have emerged with potential to mimic the extracellular matrix. The aim of the present study was to investigate the influence of the self-assembling peptide on the morphology, survival, proliferation rate, migration potential, and differentiation of human meningioma stem-like cells (hMgSCs). Materials and Methods: The efficacy of a novel method for placing hMgSCs in PuraMatrix (the injection approach) was compared to the encapsulation and surface plating methods. In addition, we designed a new method for measurement of migration distance in 3D cultivation of hMgSCs in PuraMatrix. Results: Our results revealed that hMgSCs have the ability to form spheres in stem cell culture condition. These meningioma cells expressed GFAP, CD133, vimentin, and nestin. Using the injection method, a higher proliferation rate of the hMgSCs was observed after seven days of culture. Furthermore, the novel migration assay was able to measure the migration of a single cell alone in 3D environment. Conclusion: The results indicate the injection method as an efficient technique for culturing hMgSCs in PuraMatrix. Furthermore, the novel migration assay enables us to evaluate the migration of hMgSCs. | ||
| کلیدواژهها | ||
| brain tumor؛ Cell culture؛ Cell migration؛ RADA16-I؛ Stem cells | ||
| مراجع | ||
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