Gene therapy based on interleukin-12 loaded chitosan nanoparticles in a mouse model of fibrosarcoma | ||
| Iranian Journal of Basic Medical Sciences | ||
| مقاله 13، دوره 19، شماره 11، بهمن 2016، صفحه 1238-1244 اصل مقاله (1.07 M) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22038/ijbms.2016.7825 | ||
| نویسندگان | ||
| Saiedeh Razi Soofiyani1؛ Somayeh Hallaj-Nezhadi2، 3؛ Farzaneh Lotfipour2، 3؛ Akbar Mohammad Hosseini1؛ Behzad Baradaran* 1 | ||
| 1Immonuology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran | ||
| 2Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran | ||
| 3Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran | ||
| چکیده | ||
| Objective(s): Interleukin-12 (IL-12) as a cytokine has been proved to have a critical role in stimulating the immune system and has been used as immunotherapeutic agents in cancer gene therapy. Chitosan as a polymer, with high ability of binding to nucleic acids is a good candidate for gene delivery since it is biodegradable, biocompatible and non-allergenic polysaccharide. The objective of the present study was to investigate the effects of cells transfected with IL-12 loaded chitosan nanoparticles on the regression of fibrosarcoma tumor cells (WEHI-164) in vivo. Materials and Methods: WEHI-164 tumor cells were transfected with IL-12 loaded chitosan nanoparticles and then were injected subcutaneously to inoculate tumor in BALB/c mice. Tumor volumes were determined and subsequently extracted after mice sacrifice. The immunohistochemistry staining was performed for analysis of Ki-67 expression (a tumor proliferation marker) in tumor masses. The expression of IL-12 and IFN-γ were studied using real-time polymerase chain reaction and immunoblotting. Results: The group treated with IL-12 loaded chitosan nanoparticles indicated decreasing of tumor mass volume (P<0.001). The results of western blotting and real-time PCR showed that the IL-12 expression was increased in the group. Immunohistochemistry staining indicated that the Ki-67expression was reduced in the group treated with IL-12 loaded chitosan nanoparticles. Conclusion: IL-12 gene therapy using chitosan nanoparticles has therapeutic effects on the regression of tumor masses in fibrosarcoma mouse model. | ||
| کلیدواژهها | ||
| Chitosan؛ Gene Therapy؛ IL-12؛ In vivo؛ Nanoparticle؛ Tumor | ||
| مراجع | ||
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