Involvement of microRNA-133 and -29 in cardiac disturbances in diabetic ovariectomized rats | ||
| Iranian Journal of Basic Medical Sciences | ||
| مقاله 5، دوره 19، شماره 11، بهمن 2016، صفحه 1177-1185 اصل مقاله (1.26 M) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22038/ijbms.2016.7817 | ||
| نویسندگان | ||
| Parisa Habibi1؛ Alireza Alihemmati2؛ Mohammadreza Nasirzadeh3؛ Hadi Yousefi1؛ Mohammadrasoul Habibi4؛ Nasser Ahmadiasl* 5 | ||
| 1Department of Physiology, Tabriz University of Medical Sciences, Tabriz, Iran | ||
| 2Department of Histology & Embryology, Tabriz University of Medical Sciences, Tabriz, Iran | ||
| 3Department of Physiology, Tabriz Branch, Islamic Azad University, Tabriz, Iran | ||
| 4Tabriz University of Medical Sciences, Tabriz, Iran | ||
| 5Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran | ||
| چکیده | ||
| Objective(s): Menopause and diabetes obviously increase the risk of cardiovascular disease in women. The aims of the present study were to evaluate the effects of ovariectomy in type 2 diabetes on the histology and expression of miRNA-29, miRNA-133, IGF-1 and Bcl-2 genes and Bcl-2 protein and caspase 3 activity in the hearts of female rats. Materials and Methods: Forty Female Wistar rats were divided into four groups: control, sham, ovariectomized (OVX), and ovariectomized with type 2 diabetes (OVX.D). After the 8-week experiment, the histological evaluation of the heart tissue was performed using H&E staining and PAS analysis, and cardiac expression of miRNA-29, miRNA-133, IGF-1, and Bcl-2 were evaluated using real-time PCR, and Bcl-2 protein and caspase 3 activity were evaluated using Western blot and ELISA. Results: Ovariectomy significantly decreased miRNA-29, miRNA-133, IGF-1, and BCL-2 expression and Bcl-2 protein and increased caspase 3 activity in the heart compared to sham animals group (P<0.05). Type 2 diabetes in ovariectomized rats markedly decreased expression of miRNA-29, miRNA-133, IGF-1, BCL-2 genes, and Bcl-2 protein, and increased caspase 3 activity and reduced collagen and fibroblast tissue and glycogen granule deposition in relation to OVX group (P<0.05). Conclusion: Our findings suggest that type 2 diabetes and menopause synergically could enhance the cardiac fibrosis through dysregulation of miRNA-29, miRNA-133, IGF-1, and Bcl-2 genes expression and Bcl-2 protein and upregulation of caspase 3 activity. | ||
| کلیدواژهها | ||
| Cardiac fibrosis؛ Diabetes؛ Menopause؛ MicroRNA | ||
| مراجع | ||
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