The hedgehog/Gli-1 signaling pathways is involved in the inhibitory effect of resveratrol on human colorectal cancer HCT116 cells | ||
| Iranian Journal of Basic Medical Sciences | ||
| مقاله 4، دوره 19، شماره 11، بهمن 2016، صفحه 1171-1176 اصل مقاله (1.21 M) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22038/ijbms.2016.7816 | ||
| نویسندگان | ||
| Zhou Du1؛ Feng Zhou1؛ Zengrong Jia1؛ Beishi Zheng1؛ Shaoliang Han1؛ Jun Cheng1؛ Guanbao Zhu1؛ Ping Huang* 2 | ||
| 1Department of Gastrointestinal Surgery, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325035, Zhejiang Province, China | ||
| 2Office of Development and Planning, Wenzhou Medical University, Wenzhou 325035, Zhejiang Province, China | ||
| چکیده | ||
| Objective(s): The study aimed to investigate the effects of resveratrol on colorectal cancer HCT116 cells, including cell viability, apoptosis, and migration, and the partial mechanisms focused on hedgehog/gli-1 signaling pathways. Materials and Methods: We chose the appropriate time and concentration of recombinant human Sonic hedgehog (Shh) stimulation by cell viability. The proportion of cell apoptosis was detected by flow cytometry; HCT116 cell migration was measured by scratch test; the expression of Ptch, Smo, and Gli-1 was measured by Western blot analysis. Results: Shh signaling increased HCT116 cell viability and migration, inhibited cell apoptosis, and upregulated the expression of Ptch, Smo, and Gli-1. Resveratrol obviously inhibited HCT116 cell viability and migration, promoted cell apoptosis, and suppressed the protein of Ptch, Smo, and Gli-1. Furthermore, the effects of resveratrol and Shh on human colorectal cancer HCT116 cells were in a dose- and time-dependent manner. Conclusion: The inhibitory effect of resveratrol on HCT116 cells may be mediated by hedgehog/gli-1 signaling pathways. | ||
| کلیدواژهها | ||
| Colorectal cancer؛ Hedgehog/gli-1؛ Resveratrol؛ Signaling pathways | ||
| مراجع | ||
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