Kelussia odoratissima potentiates cytotoxic effects of radiation in HeLa cancer cell line | ||
| Avicenna Journal of Phytomedicine | ||
| مقاله 6، دوره 7، شماره 2، خرداد و تیر 2017، صفحه 137-144 اصل مقاله (584.1 K) | ||
| نوع مقاله: Original Research Article | ||
| شناسه دیجیتال (DOI): 10.22038/ajp.2016.7759 | ||
| نویسندگان | ||
| Azar Hosseini1؛ Shima Saeidi Javadi2؛ Azar Fani Pakdel3؛ Seyed Hadi Mousavi* 2 | ||
| 1Pharmacological Research Center of Medicinal Plants, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran | ||
| 2Department of Pharmacology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran | ||
| 3Department of Oncology, Omid Hospitsal, Mashhad, Iran | ||
| چکیده | ||
Objective: Cervical cancer is the second most common cause of death from cancer in women throughout the world. The aim of this study was to evaluate the cytotoxic activity of Kelussia odoratissima (K. odoratissima) extract associated with radiotherapy in cervical cancer cells (HeLa cell line). Materials and Methods: Different concentration of the extract (25-500µg/ml) was tested in HeLa cell lines. Cell cytotoxicity of the extract and the effects of the extract on radiation (2Gy/min)-induced damages were assessed by MTT assay. Apoptosis was assessed using flow cytometric analysis. Result: K. odoratissima decreased cell viability in HeLa cell line in a concentration and time-dependent manner. When compared to the control,K. odoratissima induced a sub-G1 peak in the flow cytometry histogram of treated cells, indicating that apoptotic cell death is involved in K. odoratissima-induced toxicity. It was also shown that K. odoratissima sensitizes cells to radiation-induced toxicity. Conclusion: Our result showed the extract increased the radiation effect. This observation may be related to the presence of active compounds such as phthalides and ferulic acid. | ||
| کلیدواژهها | ||
| HeLa cell line؛ Kelussia odoratissima؛ Cytotoxicity؛ Radiotherapy؛ Apoptosis | ||
| مراجع | ||
|
Ahmadi F, Kadima M, Shahedi M. 2007. Antioxidant activity of Kelussia odoratissima Mozaff in model and food systems. Food chem, 105: 57–64.
Alexandre J, Batteux F, Nicco C. 2006. Accumulation of hydrogen peroxide is an early and crucial step for paclitaxel-induced cancer cell death both in vitro and in vivo. Int J Cancer, 119: 41-48.
Alexandre J, Hu Y, Lu W, Pelicano H, Huang P. 2007. Novel action of paclitaxel against cancer cells: bystander effect mediated by reactive oxygen species. Cancer Res, 67: 3512-3517.
Bandugula VR, Prasad R. 2013. 2-Deoxy-D-glucose and ferulic acid modulates radiation response signaling in non-small cell lung cancer cells. Tumor Biol, 34:251–259.
Bhoslea SM, Huilgola NG, Mishra KP. 2005. Enhancement of radiation-induced oxidative stress and cytotoxicity in tumor cells by ellagic acid. Clin Chim Acta, 359:89-100.
Bosch FX, Lorincz A, Munoz N. 2002. The casual relation between human papilloma virus and cervical cancer. J Clin Pathol, 55: 244-65.
Cragg GM, Newman DJ. 2005. Plants as a source of anti-cancer agents. J Ethnopharmacol, 100: 72-79.
Dal-Pizzol F, Ritter C, Klamt F, Andrades M, da Frota ML Jr, Diel C. 2003. Modulation of oxidative stress in response to γ-radiation in human glioma cell lines. J Neurooncol, 61:89-94.
Dewick PM. 2011. Medicinal Natural Products: A Biosynthetic Approach . West Sussex: pp: 539, John Wiley & Sons.
Dropcho EJ. 2011. The neurologic side effects of chemotherapeutic agents. Continuum, (Minneap Minn) 17: 95-112.
Dunne-Daly CF.1999. Principles of radiotherapy and radiobiology. Semin Oncol Nurs, 15:250-259.
Garg AK, Buchholz TA, Aggarwal BB. 2005. Chemosensitization and radiosensitization of tumors by plant polyphenols. Antioxid Redox Signal, 7:1630 – 1647.
Gorman A, McGowan A, Cotter TG.1997. Role of peroxide and superoxide anion during tumour cell apoptosis. FEBS Lett, 404: 27-33.
Lee KW, Lee HJ. 2006. The roles of polyphenols in cancer chemoprevention. Biofactors, 26:105 – 121.
Lopez-Lazaro M. 2008. Anticancer and carcinogenic properties of curcumin: considerations for its clinical development as a cancer chemopreventive and chemotherapeutic agent. Mol Nutr Food Res, 52 Suppl 1: S103-S127.
Lopez-Lazaro M. 2007. Dual role of hydrogen peroxide in cancer: Possible relevance to cancer chemoprevention and therapy. Cancer Lett, 252: 1-8.
Magné N, Fischel JL,Dubreuil A, Formento P, Marcié S, Lagrange JL, and Milano G. 2002. Sequence-dependent effects of ZD1839 (‘Iressa’) in combination with cytotoxic treatment in human head and neck cancer. Br J Cancer, 86: 819-827.
Monsuez J, Charniot JC, Vignat N, Artigou JY. 2010. Cardiac side-effects of cancer chemotherapy. Inter J Cardiol, 144: 3–15.
Mozaffarian V. 2003. Two new genera of Iranian Umbellifera. Bot Zhurn, 88: 88–94.
Nussbaumer S, Bonnabry P, Veuthey JL, Sandrine F. 2011. Analysis of anticancer drugs: A review. Talanta, 85: 2265-2289.
Olive PL.1998. The role of DNA single and double strand breaks in cell killing by ionizing radiation. Radiat Res, 150:S42-S51.
Pelicano H, Carney D, Huang P. 2004. ROS stress in cancer cells and therapeutic implications. Drug Resist Updat, 7: 97-110.
Qi F, Zhao L, Zhou A, Zhang B, Li A, Wang Z, Han J. 2015. The advantages of using traditional Chinese medicine as an adjunctive therapy in the whole course of cancer treatment instead of only terminal stage of cancer. BioScience Trends, 9:16-34.
Reddy BV, Prasad NR . 2011. 2-deoxy-D-glucose combined with ferulic acid enhances radiation response in non-small cell lung carcinoma cells. Cent Eur J Biol, 6:743 – 755.
Renschler MF. 2004. The emerging role of reactive oxygen species in cancer therapy. Eur J Cancer, 40: 1934-40.
Sajjadi SE, Shokoohinia Y, Moayedi NS. 2012. Isolation and Identification of Ferulic Acid From Aerial Parts of Kelussia odoratissima Mozaff. Jundishapur J Nat Pharm Prod, 7:159-162.
Schumacker PT. 2006. Reactive oxygen species in cancer cells: live by the sword, die by the sword. Cancer Cell, 10: 175-176.
She M, Pu F, Pan Z. 2005. "Apiaceae". Flora of China. Vol 14.Page1
Torres MA, Raju U, Molkentine D, Riesterer O, Milas L, Ang KK. 2011. AC480, formerly BMS-599626, a pan Her inhibitor, enhances radiosensitivity and radioresponse of head and neck squamous cell carcinoma cells in vitro and in vivo. Invest. New Drugs,29: 554-561.
Trachootham D, Alexandre J, Huang P. 2009. Targeting cancer cells by ROS-mediated mechanisms: a radical therapeutic approach? Nat Rev Drug Discov, 8:579 – 591.
Wardman P. 2007. Chemical Radiosensitizers for use in radiotherapy. Clin Oncol, 19:397-417.
Zoberi I, Bradbury CM, Curry HA. 2002. Radiosensitizing and antiproliferative effects of resveratrol in two human cervical tumor cell lines. Cancer Lett, 175:65-173. | ||
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