Anti-carcinogenic and anti-angiogenic properties of the extracts of Acorus calamus on gastric cancer cells | ||
| Avicenna Journal of Phytomedicine | ||
| مقاله 7، دوره 7، شماره 2، خرداد و تیر 2017، صفحه 145-156 اصل مقاله (635.2 K) | ||
| نوع مقاله: Original Research Article | ||
| شناسه دیجیتال (DOI): 10.22038/ajp.2016.7485 | ||
| نویسندگان | ||
| Samaneh Rahamoz Haghighi1؛ Malek Hossein Asadi* 2؛ Hassan Akrami3؛ Amin Baghizadeh2 | ||
| 1Department of Plant Breeding, Faculty of New Sciences and Technologies, Graduate University of Advanced Technology, Kerman, Iran | ||
| 2Department of Biotechnology, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran | ||
| 3Department of Biology, Faculty of Basic Science, Razi University, Kermanshah, Iran | ||
| چکیده | ||
Objective: Acorus calamus (A. calamus) has been used as a medicinal plant in Asia for its effects on digestive system for the last 2000 years. To investigate the anti-cancer activity of rhizome of A. calamus, the ethanolic and methanolic extracts and essential oil of the rhizome were prepared and their effects were assessed on human gastric cancer cell line (AGS). Materials and Methods: The viability of cells which were treated with the extracts and the essential oil was assessed by MTT assay. To evaluate the anti-angiogenic property of the extracts, in vitro tube formation assay was done. Cell cycle distribution and the expression of Oct4 and Nucleostemin, after treatments, were checked by flowcytometry and quantitative RT-PCR, respectively. Furthermore, analysis of essential oil from A.calamus was done by GC-MS. Results: Our results showed that the growth of AGS cells was inhibited by the extracts and essential oil and the extracts inhibited the angiogenesis in HUVEC cells. Our data revealed that the extracts and essential oil of A. calamus caused G1 arrest in AGS cells and downregulation of Oct4 and NS after treatment. By GC-MS analysis, we found new compoundssuch as epiprezizaene, valencene and isocyclocitral in essential oil of A. Conclusions: All together, our results showed that the extracts of A. calamus have anti-proliferative and anti-angiogenic effects on cancer cells. | ||
| کلیدواژهها | ||
| Acorus calamus؛ Gastric cancer؛ Anti-proliferative؛ Angiogenesis؛ Oct4؛ Nucleostemin | ||
| مراجع | ||
|
Agarwal SL, Arora RB, Dandiya PC, Singh KP. 1956. A note on the preliminary studies of certain pharmacological actions of Acorus calamus L. J Am Pharm Assoc Am Pharm Assoc, 45:655-656.
Asadi MH, Mowla SJ, Fathi F, Aleyasin A, Asadzadeh J, Atlasi Y. 2011. OCT4B1, a novel spliced variant of OCT4, is highly expressed in gastric cancer and acts as an antiapoptotic factor. Int J Cancer, 128:2645-2652.
Balakumbahan R, Rajamani K, Kumanan K. 2010. Acorus calamus: An overview. J Med Plants Res, 4:2740-2745.
Choi S, Park MS, Lee YR, Lee YC, Kim TW, Do SG, Kim DS, Jeon BH. 2013. A standardized bamboo leaf extract inhibits monocyte adhesion to endothelial cells by modulating vascular cell adhesion protein-1. Nutr Res Pract, 7:9-14.
Dahiru D, Sini JM, John-Africa L. 2006. Antidiarrhoeal activity of Ziziphus mauritiana root extract in rodents. Afr J Biotechnol, 5:941-945.
Devi SA, Ganjewala D. 2011. Antioxidant activities of methanolic extracts of sweet-flag (Acorus calamus) leaves and Rhizomes. J Herbs Spices Med Plants, 17:1-11.
Dorai T, Aggarwal BB. 2004. Role of chemopreventive agents in cancer therapy. Cancer Lett, 215:129-140.
Gaidhani SN, Lavekar GS, Juvekar AS, Sen S, Singh A, Kumari S. 2009. In vitro anticancer activity of standard extracts used in ayurveda. Phcog Mag, 5:425-429.
Gali-Muhtasib H, Bakkar N. 2002. Modulating cell cycle: current applications and prospects for future drug development. Curr Cancer Drug Targets, 2:309-336.
Gharaei R, Akrami H, Heidari S, Asadi MH, Jalili A. 2013. The suppression effect of Ferula gummosa Boiss. extracts on cell proliferation through apoptosis induction in gastric cancer cell line. Eur J Integr Med, 5:241–247.
Jemal A, Bray F, Center MM, Ferlay J, Ward E, Forman D. 2011. Global cancer statistics. CA Cancer J Clin, 61:69-90.
Kim KH, Moon E, Kim HK, Oh JY, Kim SY, Choi SU, Lee KR. 2012. Phenolic constituents from the rhizomes of Acorus gramineus and their biological evaluation on antitumor and anti-inflammatory activities. Bioorg Med Chem Lett, 22:6155-6159.
Kumar A, Kumar P, Kumar V, Kumar M. 2014. Traditional uses of wetland medicinal plant Acorus calamus: review and perspectives. Int J Referred Online Res, 17:37-67.
Malekzadeh R, Derakhshan MH, Malekzadeh Z. 2009. Gastric cancer in Iran: epidemiology and risk factors. Arch Iran Med, 12:576-583.
Marcy J, Balunas AA, Douglas KB. 2005. Drug discovery from medicinal plants. Life Sci, 78:431-441.
Meena AK, Rao MM, Singh A, Kumari S. 2010. Phytochemical and preliminary phytochemical studies on the rhizome of Acorus calamus Linn. Int J Pharm Pharm Sci, 2:130-131.
Mehrotra S, Mishra KP, Maurya R, Srimal RC, Yadav VS, Pandey R, Singh VK. 2003. Anticellular and immunosuppressive properties of ethanolic extract of Acorus calamus rhizome. Int Immunopharmacol, 3:53-61.
Mukherjee PK, Kumar V, Mal M, Houghton PJ. 2007. Acorus calamus: Scientific validation of ayurvedic tradition from natural resources. Pharm Biol, 45:651–666.
Nishiyama N, Zhou Y, Saito H. 1994. Ameliorative effects of chronic treatment using DX-9386, a traditional Chinese prescription, on learning performance and lipid peroxide content in senescence accelerated mouse. Biol Pharm Bull, 17:1481-1484.
Palani S, Raja S, Praveen kumar R, Venkadesan D, Devi K, Sivaraj A, Senthilkumar B. 2009. Therapeutic efficacy of antihepatotoxic and antioxidant activities of Acorus calamus on acetaminophen induced toxicity in rat. Int J Int Biol, 7:39-44.
Powis G. 1983. Dose-dependent metabolism, therapeutic effect, and toxicity of anticancer drugs in man. Drug Metab Rev, 14:1145-1163.
Rajkumar V, Gunjan G, Ashok KR, Lazar M. 2009. Evaluation of Cytotoxic Potential of Acorus calamus Rhizome. Ethnobotanical Leaflets, 13:832-839.
Rajput SB, Karuppayil SM. 2013. beta-Asarone, an active principle of Acorus calamus rhizome, inhibits morphogenesis, biofilm formation and ergosterol biosynthesis in Candida albicans. Phytomedicine, 20:139-142.
Samaneh Rahamoz- Haghighi MHA, Ali Riahi- madvar, Amin Baghizadeh. 2014. Antibacterial effect of Acorus calamus extractions against gram positive and negative bacteria. J Ethno-Pharma products, 1:1-7.
Singh R, Sharma PK, Malviya R. 2011. Pharmacological Properties and Ayurvedic Value of Indian Buch Plant (Acorus calamus): A Short Review Adv Biol Res, 5:145-154.
Tiwari N, Chaudhary A, Mishra A, Bhatt G. 2010. Anti-inflammatory effects of the saponins obtained from the leaves of Acorus Calamus. Pharmacologyonline, 2:395-400.
Zhang Y, Takashina K, Saito H, Nishiyama N. 1994. Anti-aging effect of DX-9386 in senescence accelerated mouse. Biol Pharm Bull, 17:866-868.
| ||
|
آمار تعداد مشاهده مقاله: 16,312 تعداد دریافت فایل اصل مقاله: 6,935 |
||