Antinociceptive and anti-inflammatory effects of Urtica dioica leaf extract in animal models | ||
| Avicenna Journal of Phytomedicine | ||
| مقاله 11، دوره 3، شماره 2، خرداد 2013، صفحه 193-200 اصل مقاله (213.84 K) | ||
| نوع مقاله: Original Research Article | ||
| شناسه دیجیتال (DOI): 10.22038/ajp.2013.73 | ||
| نویسندگان | ||
| Valiollah Hajhashemi* ؛ Vahid Klooshani | ||
| Department of Pharmacology and Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, I. R. Iran | ||
| چکیده | ||
| Objective: This study was aimed to examine the antinociceptive and anti-inflammatory effects of Urtica dioica leaf extract in animal models. Materials and Methods: Hydroalcoholic extract of the plant leaves was prepared by percolation method. Male Swiss mice (25-35 g) and male Wistar rats (180-200 g) were randomly distributed in control, standard drug, and three experimental groups (n=6 in each group). Acetic acid-induced writhing, formalin test, and carrageenan-induced paw edema were used to assess the antinociceptive and anti-inflammatory effects. Results: The extract dose-dependently reduced acetic acid-induced abdominal twitches. In formalin test, the extract at any of applied doses (100, 200, and 400 mg/kg) could not suppress the licking behavior of first phase while doses of 200 and 400 mg/kg significantly inhibited the second phase of formalin test. In carrageenan test, the extract at a dose of 400 mg/kg significantly inhibited the paw edema by 26%. Conclusion: The results confirm the folkloric use of the plant extract in painful and inflammatory conditions. Further studies are needed to characterize the active constituents and the mechanism of action of the plant extract. | ||
| کلیدواژهها | ||
| Anti-inflammatory؛ Antinociceptive؛ Extract؛ Urtica dioica | ||
| مراجع | ||
|
Amresh G, Reddy GD, Rao C, Singh PN. 2007. Evaluation of anti-inflammatory activity of Cissampelos pareira root in rats. J Ethnopharmacol,110: 526-531.
Balch JF, Balch PA. 2000. Prescription for Nutritional Healing. p. 104,Garden City Park, Avery Publishing Group.
Bisser NG. 1994. Herbal Drugs and Polypharmaceuticals, pp. 505-509, Boca Raton, CRC Press.
Bors W, Saran M. 1987. Radical scavenging by flavonoid antioxidants. Free Radic Res Commun, 2: 289-294.
Chandler F. 2000. Herbs, Everyday Reference for Health Professionals, pp. 206-207, Canada, Canadian Pharmacists Association and Canadian Medical Association.
Chen YF, Tsai HY, Wu TS. 1995. Anti-inflammatory and analgesic activities from roots of Angelica pubescens. Planta Med, 61: 2-8.
Collier HO, Dinneen LC, Johnson CA, Schneider C. 1968. The abdominal constriction response and its suppression by analgesic drugs in the mouse. Br J Pharmacol Chemother, 32: 295-310.
Daher CF, Baroody KG, Baroody GM. 2006. Effect of Urtica dioica extract intake upon blood lipid profile in the rats. Fitoterapia77: 183-188.
Dar SA, Ganai FA, Yousuf AR, Balkhi MU, Bhat TM, Sharma P. 2012. Pharmacological and toxicological evaluation of Urtica dioica. Pharm Biol, [in press].
El Haouari M, Bnouham M, Bendahou M, Aziz M, Ziyyat A, Legssyer A, Mekhfi H. 2006. Inhibition of rat platelet aggregation by Urtica dioica leaves extracts. Phytother Res,20: 568-572.
Elisabetsky E, Amador TA, Albuquerque RR, Nunes DS, Carvalho AC. 1995. Analgesic activity of Psychotria colorata (Willd. ex R. & S.) Muell. Arg. alkaloids. J Ethnopharmacol, 48: 77-83.
Ghannadi A, Hajhashemi V, Jafarabadi H. 2005. An investigation of the analgesic and anti-inflammatory effects of Nigella sativa seed polyphenols. J Med Food, 8: 488-493.
Grieve M. 1971. A Modern Herbal, Vol 2. pp. 574-579, New York, Dover Publications.
Hajhashemi V, Ghannadi A, Sharif B. 2003. Anti-inflammatory and analgesic properties of the leaf extracts and essential oil of Lavandula angustifolia Mill. J Ethnopharmacol, 89: 67-71.
Hajhashemi V, Sajjadi SE, Heshmati M. 2009. Anti-inflammatory and analgesic properties of Heracleum persicum essential oil and hydroalcoholic extract in animal models. J Ethnopharmacol, 124: 475-480.
Hajhashemi V, Sajjadi SE, Zomorodkia M. 2011. Antinociceptive and anti-inflammatory activities of Bunium persicum essential oil, hydroalcoholic and polyphenolic extracts in animal models. Pharm Biol, 49: 146-151.
Hirano T, Homma M, Oka K. 1994. Effects of stinging nettle root extracts and their steroidal components on the Na+, K(+)-ATPase of the benign prostatic hyperplasia. Planta Med, 60: 30-33.
Hryb DJ, Khan MS, Romas NA, Rosner W. 1995. The effect of extracts of the roots of the stinging nettle (Urtica dioica) on the interaction of SHBG with its receptor on human prostatic membranes. Planta Med, 61: 31-32.
Hunskaar S, Hole K. 1987. The formalin test in mice: dissociation between inflammatory and non-inflammatory pain. Pain, 30: 103-114.
Iranian Herbal Pharmacopoea Committee. 2003. Iranian Herbal Pharmacopoea. pp. 680-686, Tehran, Ministry of Health and Medical Education Publishing
Koster R, anderson M, De Beer EJ. 1959. Acetic acid for analgesic screening . Fed Proc, 18: 417.
Marrassini C, Acevedo C, Miño J, Ferraro G, Gorzalczany S. 2010. Evaluation of antinociceptive, antinflammatory activities and phytochemical analysis of aerial parts of Urtica urens L. Phytother Res, 24: 1807-1812
Newall CA, Anderson LA, Phillipson JD. 1996. Herbal Medicines. pp. 201-202, London, The Pharmaceutical Press.
Obertreis B, Giller K, Teucher T, Behnke B, Schmitz H. 1996. Antiinflammatory effect of Urtica dioica folia extract in comparison to caffeic malic acid. Arzneimittelforschung, 46: 52-56.
Safarinejad MR. 2005. Urtica dioica for treatment of benign prostatic hyperplasia: prospective, randomized, double-blind, placebo-controlled, crossover study. J Herb Pharmacother, 5: 1-11.
Sajjadi SE, Movahedian-Atar AM, Yektaian A. 1998. Antihyperlipidemic effect of hydroalcoholic extract and polyphenolic fraction from Dracocephalum Kotschyi Boiss. Pharm Acta Helv, 73: 167-170.
Schottner M, Gansser D, Spiteller G. 1997. Lignans from the roots of Urtica dioica and their metabolites bind to human sex hormone binding globulin (SHBG). Planta Med, 63: 529-532.
Tjolsen A, Berge OG, Hunskaar S, Rosland JH, Hole K. 1992. The formalin test: an evaluation of the method. Pain, 51: 5-17.
Vogel HG, Vogel WH. 1997. Drug Discovery and Evaluation. pp. 382-383, Berlin, Springer.
| ||
|
آمار تعداد مشاهده مقاله: 28,198 تعداد دریافت فایل اصل مقاله: 6,265 |
||