Histomorphometric evaluation of treatment of rat azoosper-mic seminiferous tubules by allotransplantation of bone marrow-derived mesenchymal stem cells | ||
| Iranian Journal of Basic Medical Sciences | ||
| مقاله 11، دوره 19، شماره 6، شهریور 2016، صفحه 653-661 اصل مقاله (1.29 M) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22038/ijbms.2016.7134 | ||
| نویسندگان | ||
| Farhad Rahmanifar1؛ Amin Tamadon2؛ Davood Mehrabani* 2؛ Shahrokh Zare2؛ Sorush Abasi2؛ Saeideh Keshavarz2؛ Mehdi Dianatpour2، 3؛ Zahra Khodabandeh2؛ Iman Raze ghian Jahromi4؛ Omid Koohi-Hoseinabadi5 | ||
| 1Department of Basic Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran | ||
| 2Stem Cell and Transgenic Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran | ||
| 3Department of Medical Genetics, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran | ||
| 4Cardiovascular Research Center, Shiraz University of Medical Sciences, Shiraz, Iran | ||
| 5Laboratory Animal Center, Shiraz University of Medical Sciences, Shiraz, Iran | ||
| چکیده | ||
| Objective(s): Bone marrow-derived mesenchymal stem cells (BM-MSCs) potentials make them appropriate for cell therapy including ability of differentiation and release of anti-inflammatory cytokines and growth factors secreta. For treatment of azoospermia to induce proliferation and differentiation of germ cells, MSCs transplantation has been introduced. The aim of the present experimental case-control study was to histomorphometric evaluation of the germinal cells in seminiferous tubules of azoospermic rats before and after BM-MSCs allotransplantation. Materials and Methods: In the present study, BM-MSCs were isolated from six male rats and confirmed. Their testes also served as intact negative controls. The recipient rats (n=6) were received two doses of 10 mg/kg of busulfan with 21 days interval to induce azoospermia. After cessation of spermatogenesis, the rats were allotransplanted with the BM-MSCs into efferent duct of right testes. Thirty-five days later, the right cell-treated testes were compared to left azoospermic ones. Results: Histomorphometric analyses showed that the seminiferous tubules treated with BM-MSCs had normal morphology in comparison with azoospermic testes, which were without germinal layer. In most BM-MSCs-treated seminiferous tubules, spermatogenesis was observed. Conclusion: The allotransplanted BM-MSCs could induce spermatogenesis in seminiferous tubules of azoospermic rats. | ||
| کلیدواژهها | ||
| Azoospermia؛ Bone marrow mesenchymal- stem cell؛ Busulfan؛ Cell therapy؛ Rat | ||
| مراجع | ||
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