Nikpou, Parisa, Mohammad Nejad, Daryoush, Shafaei, Hajar, Roshangar, Leila, Samadi, Nasser, Navali, Amir Mohammad, Sadegpour, Ali Reza, Shanehbandi, Dariush, Soleimani Rad, Jafar. (1395). Study of chondrogenic potential of stem cells in co-culture with chondrons. , 19(6), 638-645. doi: 10.22038/ijbms.2016.7132
Parisa Nikpou; Daryoush Mohammad Nejad; Hajar Shafaei; Leila Roshangar; Nasser Samadi; Amir Mohammad Navali; Ali Reza Sadegpour; Dariush Shanehbandi; Jafar Soleimani Rad. "Study of chondrogenic potential of stem cells in co-culture with chondrons". , 19, 6, 1395, 638-645. doi: 10.22038/ijbms.2016.7132
Nikpou, Parisa, Mohammad Nejad, Daryoush, Shafaei, Hajar, Roshangar, Leila, Samadi, Nasser, Navali, Amir Mohammad, Sadegpour, Ali Reza, Shanehbandi, Dariush, Soleimani Rad, Jafar. (1395). 'Study of chondrogenic potential of stem cells in co-culture with chondrons', , 19(6), pp. 638-645. doi: 10.22038/ijbms.2016.7132
Nikpou, Parisa, Mohammad Nejad, Daryoush, Shafaei, Hajar, Roshangar, Leila, Samadi, Nasser, Navali, Amir Mohammad, Sadegpour, Ali Reza, Shanehbandi, Dariush, Soleimani Rad, Jafar. Study of chondrogenic potential of stem cells in co-culture with chondrons. , 1395; 19(6): 638-645. doi: 10.22038/ijbms.2016.7132

Study of chondrogenic potential of stem cells in co-culture with chondrons

Iranian Journal of Basic Medical Sciences
مقاله 9، دوره 19، شماره 6، 2016، صفحه 638-645    اصل مقاله (1.1 M)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2016.7132
نویسندگان
Parisa Nikpou1؛ Daryoush Mohammad Nejad2؛ Hajar Shafaei1؛ Leila Roshangar1؛ Nasser Samadi3؛ Amir Mohammad Navali4؛ Ali Reza Sadegpour4؛ Dariush Shanehbandi5؛ Jafar Soleimani Rad* 1، 6
1Department of Anatomical Sciences, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
2Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
3Department of Biochemistry and Laboratory Sciences, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran
4Department of Orthopedy, Tabriz University of Medical Sciences, Tabriz, Iran
5Department of Immunolog, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
6Umblical Cord Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
چکیده
Objective(s): Three-dimensional biomimetic scaffolds have widespread applications in biomedical tissue engineering due to similarity of their nanofibrous architecture to native extracellular matrix. Co-culture system has stimulatory effect on chondrogenesis of adult mesenchymal stem cells. This work presents a co-culture strategy using human articular chondrons and adipose-derived stem cells (ASCs) from infrapatellar fat pad (IPFP) for cartilage tissue production.
Materials and Methods: Isolated stem cells were characterized by flowcytometry. Electrospun and polycaprolactone (PCL) scaffolds (900 nm fiber diameter) was obtained from Bon Yakhteh (Tehran- Iran) and human infrapatellar fat pad-derived stem cells (IPFP-ASCs) were seeded on them. IPFP- ASCs on scaffolds were co-cultured with articular chondrons using transwell. After 21 day, chondrogenic differentiation of stem cell was evaluated by determining the genes expression of collagen2, aggrecan and Indian hedgehog using real- time RT-PCR.
Results: Genes expression of collagen2, aggrecan by IPFP-ASCs did not alter significantly in comparison with control group. Howevers, expression of Indian hedgehog decreased significantly compared to control group (P˂ 0.05).
Conclusion: These findings indicate that chondrons obtained from osteoarthritic articular cartilage did not stimulate chondrogenic differentiation of IPFP-ASCs in co-culture.
کلیدواژه‌ها
Chondron؛ Co-culture؛ Nanofiber؛ Poly-e-caprolactone scaffold
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