Effect of ischemic preconditioning on the expression of c-myb in the CA1 region of the gerbil hippocampus after ischemia/reperfusion injury

Young Lee, Hui; Tae, Hyun-Jin; Cho, Geum-Sil; Kim, In Hye; Hwi Cho, Jeong; Ha Park, Joon; Hyeon Ahn, Ji; Hui Chen, Bai; Shin, Bich-Na; Won, Moo-Ho; Woo Park, Chan; Hwi Cho, Jun; Yeol Seo, Jeong; Lee, Jae-Chul

doi:10.22038/ijbms.2016.7130

	Effect of ischemic preconditioning on the expression of c-myb in the CA1 region of the gerbil hippocampus after ischemia/reperfusion injury
Iranian Journal of Basic Medical Sciences
مقاله 7، دوره 19، شماره 6، 2016، صفحه 624-631 اصل مقاله (1.39 M)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2016.7130
نویسندگان
Hui Young Lee¹؛ Hyun-Jin Tae²؛ Geum-Sil Cho³؛ In Hye Kim⁴؛ Jeong Hwi Cho⁴؛ Joon Ha Park⁴؛ Ji Hyeon Ahn²؛ Bai Hui Chen⁵؛ Bich-Na Shin⁵؛ Moo-Ho Won⁴؛ Chan Woo Park⁶؛ Jun Hwi Cho⁶؛ Jeong Yeol Seo⁷؛ Jae-Chul Lee^* ⁴
¹Department of Internal Medicine, School of Medicine, Kangwon National University, Chuncheon 200-701, South Korea
²Department of Biomedical Science, Research Institute of Bioscience and Biotechnology, Hallym University, Chuncheon 200-702, South Korea
³Pharmacology & Toxicology Department, Shinpoong Pharmaceutical Co., Ltd., Ansan 425-100, South Korea
⁴Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon 200-701, South Korea
⁵Department of Physiology, College of Medicine, Hallym University, Chuncheon 200-702, South Korea
⁶Department of Emergency Medicine, School of Medicine, Kangwon National University, Chuncheon 200-701, South Korea
⁷Department of Emergency Medicine, Chuncheon Sacred Heart Hospital, College of Medicine, Hallym University, Chuncheon 200-702, South Korea
چکیده
*Objective(s):* In the present study, we investigated the effect of ischemic preconditioning (IPC) on c-myb immunoreactivity as well as neuronal damage/death after a subsequent lethal transient ischemia in gerbils. *Materials and Methods:* IPC was subjected to a 2 min sublethal ischemia and a lethal transient ischemia was given 5 min transient ischemia. The animals in all of the groups were given recovery times of 1 day, 2 days and 5 days and we examined change in c-myb immunoreactivity as well as neuronal damage/death in the hippocampus induced by a lethal transient ischemia. *Results:A lethal transient ischemia induced a significant loss of cells in the stratum pyramidale (SP) of the hippocampal CA1 region at 5 days post-ischemia, and this insult showed that c-myb immunoreactivity in cells of the SP of the CA1 region was significantly decreased at 2 days post-ischemia and disappeared at 5 days post-ischemia. However, IPC effectively prevented the neuronal loss in the SP and showed that c-myb immunoreactivity was constitutively maintained in the SP after a lethal transient ischemia. Conclusion:* Our results show that a lethal transient ischemia significantly decreased c-myb immunoreactivity in the SP of the CA1 region and that IPC well preserved c-myb immunoreactivity in the SP of the CA1 region. We suggest that the maintenance of c-myb might be related with IPC-mediated neuroprotection after a lethal ischemic insult.
کلیدواژه‌ها
c-myb؛ Cells in stratum pyramidale؛ Delayed neuronal death؛ Ischemic preconditioning؛ Ischemia-reperfusion؛ protection
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