Effect of ischemic preconditioning on the expression of c-myb in the CA1 region of the gerbil hippocampus after ischemia/reperfusion injury | ||
| Iranian Journal of Basic Medical Sciences | ||
| مقاله 7، دوره 19، شماره 6، شهریور 2016، صفحه 624-631 اصل مقاله (1.39 M) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22038/ijbms.2016.7130 | ||
| نویسندگان | ||
| Hui Young Lee1؛ Hyun-Jin Tae2؛ Geum-Sil Cho3؛ In Hye Kim4؛ Jeong Hwi Cho4؛ Joon Ha Park4؛ Ji Hyeon Ahn2؛ Bai Hui Chen5؛ Bich-Na Shin5؛ Moo-Ho Won4؛ Chan Woo Park6؛ Jun Hwi Cho6؛ Jeong Yeol Seo7؛ Jae-Chul Lee* 4 | ||
| 1Department of Internal Medicine, School of Medicine, Kangwon National University, Chuncheon 200-701, South Korea | ||
| 2Department of Biomedical Science, Research Institute of Bioscience and Biotechnology, Hallym University, Chuncheon 200-702, South Korea | ||
| 3Pharmacology & Toxicology Department, Shinpoong Pharmaceutical Co., Ltd., Ansan 425-100, South Korea | ||
| 4Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon 200-701, South Korea | ||
| 5Department of Physiology, College of Medicine, Hallym University, Chuncheon 200-702, South Korea | ||
| 6Department of Emergency Medicine, School of Medicine, Kangwon National University, Chuncheon 200-701, South Korea | ||
| 7Department of Emergency Medicine, Chuncheon Sacred Heart Hospital, College of Medicine, Hallym University, Chuncheon 200-702, South Korea | ||
| چکیده | ||
| Objective(s): In the present study, we investigated the effect of ischemic preconditioning (IPC) on c-myb immunoreactivity as well as neuronal damage/death after a subsequent lethal transient ischemia in gerbils. Materials and Methods: IPC was subjected to a 2 min sublethal ischemia and a lethal transient ischemia was given 5 min transient ischemia. The animals in all of the groups were given recovery times of 1 day, 2 days and 5 days and we examined change in c-myb immunoreactivity as well as neuronal damage/death in the hippocampus induced by a lethal transient ischemia. Results:A lethal transient ischemia induced a significant loss of cells in the stratum pyramidale (SP) of the hippocampal CA1 region at 5 days post-ischemia, and this insult showed that c-myb immunoreactivity in cells of the SP of the CA1 region was significantly decreased at 2 days post-ischemia and disappeared at 5 days post-ischemia. However, IPC effectively prevented the neuronal loss in the SP and showed that c-myb immunoreactivity was constitutively maintained in the SP after a lethal transient ischemia. Conclusion: Our results show that a lethal transient ischemia significantly decreased c-myb immunoreactivity in the SP of the CA1 region and that IPC well preserved c-myb immunoreactivity in the SP of the CA1 region. We suggest that the maintenance of c-myb might be related with IPC-mediated neuroprotection after a lethal ischemic insult. | ||
| کلیدواژهها | ||
| c-myb؛ Cells in stratum pyramidale؛ Delayed neuronal death؛ Ischemic preconditioning؛ Ischemia-reperfusion؛ protection | ||
| مراجع | ||
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