Evaluation of infection course in mice induced by L. major in presence of positively charged liposomes containing CpG ODN | ||
| Nanomedicine Journal | ||
| مقاله 4، دوره 1، شماره 1، دی 2013، صفحه 28-37 اصل مقاله (844.11 K) | ||
| نوع مقاله: Research Paper | ||
| شناسه دیجیتال (DOI): 10.7508/nmj.2013.01.004 | ||
| نویسندگان | ||
| Hesamoddin Hoseinjani1؛ Mahmoud Reza Jaafari1، 2؛ Ali Khamesipour3؛ Azam Abbasi1؛ Zahra Saberi1؛ Ali Badiee* 1 | ||
| 1Nanotechnology Research Center, School of Pharmacy, Mashhad University of Medical Science, Mashhad, Iran | ||
| 2Biotechnology Research Center, School of Pharmacy, Mashhad University of Medical Science, Mashhad, Iran | ||
| 3Center for Research and Training in Skin Diseases and Leprosy, Tehran University of Medical Sciences, Tehran, Iran | ||
| چکیده | ||
| An inoculation of virulent Leishmania major is known as leishmanization (LZ) which is proven to be the most effective control measure against Cutaneous Leishmaniasis (CL). However, using LZ is restricted due to various side effects such as uncontrolled lesion development. In the present research, the efficacy of cationic nanoliposomes containing CpG oligodeoxynucleotides (CpG ODN) as an improved adjuvant delivery system was studied to diminish the lesion development and infection course of L. major after inoculation into the mice. BALB/c mice were inoculated subcutaneously (SC) with L. major plus empty DSPC, DSPC (CpG ODN), DSPC (Non CpG ODN), empty DMPC, DMPC (CpG ODN), DMPC (Non CpG ODN) or HEPES buffer. The results showed that group of mice received DMPC (CpG ODN) nanoliposomes developed a significantly smaller lesion and showed minimum number of L. major in the spleen and draining lymph nodes. In addition, using DMPC (CpG ODN) liposomes resulted in a Th1 type of immune response with a preponderance of IgG2a isotype which is concurrent with the production of DMPC (CpG) induced IFN-γ in the spleen of the mice. Taken together, the results suggested that immune modulation using DMPC (CpG ODN) nanoliposomes might be a practical approach to improve the safety of LZ | ||
| کلیدواژهها | ||
| DMPC (CpG ODN) nanoliposomes؛ CpG ODN؛ L. major؛ Leishmanization؛ Immune response | ||
| مراجع | ||
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