The protective effects of epigallocatechin gallate on lipopolysa ccharide-induced hepatotoxicity: an in vitro study on Hep3B cells | ||
| Iranian Journal of Basic Medical Sciences | ||
| مقاله 4، دوره 19، شماره 5، مرداد 2016، صفحه 483-489 اصل مقاله (977.71 K) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22038/ijbms.2016.6932 | ||
| نویسندگان | ||
| Murat Karamese* 1؛ Bulent Guvendi2؛ Selina Aksak Karamese3؛ Irfan Cinar4؛ Serpil Can5؛ Huseyin Serkan Erol6؛ Hakan Aydin7؛ Volkan Gelen8؛ Emre Karakus9 | ||
| 1Department of Medical Microbiology, Medical Faculty, Kafkas University, Kars, Turkey | ||
| 2Department of General Surgery, Medical Faculty, Kafkas University, Kars, Turkey | ||
| 3Department of Histology and Embryology, Medical Faculty, Kafkas University, Kars, Turkey | ||
| 4Department of Pharmacology, Medical Faculty, Ataturk University, Erzurum, Turkey | ||
| 5Department of Physiology, Medical Faculty, Kafkas University, Kars, Turkey | ||
| 6Department of Biochemistry, Veterinary Faculty, Ataturk University, Erzurum, Turkey | ||
| 7Department of Virology, Veterinary Faculty, Ataturk University, Erzurum, Turkey | ||
| 8Department of Physiology, Veterinary Faculty, Kafkas University, Kars, Turkey | ||
| 9Department of Pharmacology and Toxicology, Veterinary Faculty, Ataturk University, Erzurum, Turkey | ||
| چکیده | ||
| Objective(s): In the present study, our aim was to investigate the possible protective effects of epigallocatechin gallate (EGCG) on lipopolysaccharide (LPS)-induced hepatotoxicity by using Hep3B human hepatoma cells. Specifically, the study examines the role of some proinflammatory markers and oxidative damage as possible mechanisms of LPS-associated cytotoxicity. Consequently, the hepatocellular carcinoma cell line Hep3B was chosen as a model for investigation of LPS toxicity and the effect of EGCG on LPS-exposed cells. Materials and Methods:The Hep3B human hepatoma cells were used for this study. The cytotoxic effects of chemicals (EGCG and LPS), AST and ALT levels, SOD and CAT activities, GSH-Px level and TNF-alpha and IL-6 levels were detected by using different biochemical and molecular methods. LPS and EGCG were applied to cells at various times and doses. Results:The highest treatment dose of EGCG (400 µM) led to a dramatic decrease in SOD level and increase in CAT and GSH levels. Additionally, the highest dose of EGCG also led to a dramatic increase in TNF-alpha and IL-6 levels. On the other hand, effective doses of EGCG (200 and 100 µM) normalized all related parameters levels. Conclusion:LPS caused hepatotoxicity, but interestingly, a high dose of EGCG was found to be a cytotoxic agent in this study. However, other two doses of EGCG led to a decrease in both inflammatory cytokine levels and antioxidant enzyme levels. Further studies should examine the effect of EGCG on secondary cellular signaling pathways. | ||
| کلیدواژهها | ||
| Cytokines؛ Epigallocatechin gallate؛ Hep3B؛ Lipopolysaccharide Oxidative stress | ||
| مراجع | ||
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