سامانه مدیریت نشریات علمی دانشگاه علوم پزشکی مشهد

Naghashpour, Mahshid, Amani, Reza, Sarkaki, Alireza, Ghadiri, Ata, Samarbafzadeh, Alireza, Jafarirad, Sima, Saki Malehi, Amal. (1395). Brain-derived neurotrophic and immunologic factors: beneficial effects of riboflavin on motor disability in murine model of multiple sclerosis. , 19(4), 439-448. doi: 10.22038/ijbms.2016.6817
Mahshid Naghashpour; Reza Amani; Alireza Sarkaki; Ata Ghadiri; Alireza Samarbafzadeh; Sima Jafarirad; Amal Saki Malehi. "Brain-derived neurotrophic and immunologic factors: beneficial effects of riboflavin on motor disability in murine model of multiple sclerosis". , 19, 4, 1395, 439-448. doi: 10.22038/ijbms.2016.6817
Naghashpour, Mahshid, Amani, Reza, Sarkaki, Alireza, Ghadiri, Ata, Samarbafzadeh, Alireza, Jafarirad, Sima, Saki Malehi, Amal. (1395). 'Brain-derived neurotrophic and immunologic factors: beneficial effects of riboflavin on motor disability in murine model of multiple sclerosis', , 19(4), pp. 439-448. doi: 10.22038/ijbms.2016.6817
Naghashpour, Mahshid, Amani, Reza, Sarkaki, Alireza, Ghadiri, Ata, Samarbafzadeh, Alireza, Jafarirad, Sima, Saki Malehi, Amal. Brain-derived neurotrophic and immunologic factors: beneficial effects of riboflavin on motor disability in murine model of multiple sclerosis. , 1395; 19(4): 439-448. doi: 10.22038/ijbms.2016.6817

Brain-derived neurotrophic and immunologic factors: beneficial effects of riboflavin on motor disability in murine model of multiple sclerosis

Iranian Journal of Basic Medical Sciences
مقاله 14، دوره 19، شماره 4، تیر 2016، صفحه 439-448    اصل مقاله (1.21 M)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2016.6817
نویسندگان
Mahshid Naghashpour1، 2؛ Reza Amani3، 2؛ Alireza Sarkaki* 4، 5؛ Ata Ghadiri6، 7؛ Alireza Samarbafzadeh8، 9؛ Sima Jafarirad1، 2؛ Amal Saki Malehi10
1Nutrition and Metabolic Diseases Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Khuzestan, Iran
2Department of Nutrition, Faculty of Para-Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Khuzestan, Iran
3Health Research Institute, Diabetes Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Khuzestan, Iran
4Physiology Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Khuzestan, Iran
5Department of Physiology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Khuzestan, Iran
6Cell and Molecular Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Khuzestan, Iran
7Department of Immunology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Khuzestan, Iran
8Infectious and Tropical Disease Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Khuzestan, Iran
9Department of Virology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Khuzestan, Iran
10Department of Vital Statistics, Faculty of Health, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Khuzestan, Iran
چکیده
Objective(s): In the present study, C57BL/6 female mice (n=56) were used to explore the neuroprotective effects of riboflavin in motor disability of experimental autoimmune encephalomyelitis (EAE) as a model of multiple sclerosis.
Materials and Methods: The animals were assigned into 7 groups: sham-operated 1 (SO1), healthy mice receiving PBS (phosphate buffer saline); sham-operated 2 (SO2), healthy mice receiving PBS and riboflavin; sham treatment 1 (ST1), EAE mice receiving water; sham treatment 2 (ST2), EAE mice receiving sodium acetate buffer; treatment 1 (T1), EAE mice receiving interferon beta-1a (INFβ-1a); treatment 2 (T2), EAE mice receiving riboflavin; treatment 3 (T3), EAE mice receiving INFβ-1a and riboflavin. After EAE induction, scoring was performed based on clinical signs. Upon detecting score 0.5, riboflavin at 10 mg/kg of body weight and/or INFβ-1a at 150 IU/g of body weight administration was started for two weeks. The brain and spinal cord levels of brain-derived neurotrophic factor (BDNF), interleukin-6 (IL-6), and interleukin-17A (IL-17A) were studied using real-time PCR and ELISA methods.
Results: BDNF expression and protein levels were increased in the brain and spinal cord of the T3 group compared with the other groups (P<0.01). IL-6 and IL-17A expressions were increased in the brains of the T3 and T1 groups, respectively, compared to the other groups (P<0.01). The daily clinical score was reduced significantly by riboflavin in both effector and chronic phases of the disease compared with that of the controls (P<0.05).
Conclusion: Our findings showed that riboflavin is capable of suppressing the neurological disability mediated by BDNF and IL-6.
کلیدواژه‌ها
Brain-derived neurotrophic -factor؛ Experimental autoimmune -encephalomyelitis؛ Interleukin-17A؛ Interleukin-6؛ Motor disability؛ Riboflavin
مراجع
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