Tracheal overexpression of IL-1β, IRAK-1 and TRAF-6 mRNA in obese-asthmatic male Wistar rats | ||
| Iranian Journal of Basic Medical Sciences | ||
| مقاله 2، دوره 19، شماره 4، تیر 2016، صفحه 350-357 اصل مقاله (329.39 K) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22038/ijbms.2016.6805 | ||
| نویسندگان | ||
| Mohammad Reza Aslani1؛ Rana Keyhanmanesh2؛ Amir Mehdi Khamaneh3؛ Mehran Mesgari Abbasi2؛ Maryam Fallahi4؛ Mohammad Reza Alipour* 1 | ||
| 1Tuberculosis and Lung Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran | ||
| 2Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran | ||
| 3School of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran | ||
| 4Department of Physiology, Tabriz University of Medical Sciences, Tabriz, Iran | ||
| چکیده | ||
| Objective(s): Human and animal studies have shown a close relationship between obesity and asthma severity. Here, we examined the effects of diet-induced obesity (DIO) on the expression levels of IL-1β, IRAK-1 and TRAF-6 mRNA in male Wistar rats tracheal after sensitization with ovalbumin (OVA). Materials and Methods:Twenty male Wistar rats divided to four groups, included, control group with normal diet (C+ND), OVA-sensitized group with normal diet (S+ND), control group with high-fat diet (C+HFD), and OVA-sensitized group with high-fat diet (S+HFD). All animals fed for 8 weeks with standard pelts or high-fat diet, and then were sensitized and challenged with OVA or saline for another 4 weeks with designed regimens. At the end of study, trachea isolated and examined for expression levels of IL-1β, IRAK-1 and TRAF-6 mRNA with RT-PCR method. Results:Diet-induced obesity groups developed increased weight, obesity indexes and lipid profiles (P<0.05 to P<0.001). The expression levels of IL-1β mRNA in OVA-sensitization groups (S+ND and S+HFD) showed a significantly increased when compared with control group. Also in S+HFD group, expression level of TRAF-6 mRNA was higher than other groups (P<0.001). IRAK-1 expression level was high in S+HFD compared with control group.IL-1β and TRAF-6 mRNA correlated positively with obesity indexes. Conclusion:The results showed that DIO causes overexpression of IL-1β, IRAK-1 and TRAF-6 mRNA in an experimental model of asthma. Our results suggested that in obese-asthmatic conditions locally production and activation of pro-inflammatory agents can be increased. These findings showed that possible mechanism for obesity-asthma relationships. | ||
| کلیدواژهها | ||
| Asthma؛ IL-1β؛ IRAK-1؛ Obesity؛ Tracheal؛ TRAF-6 | ||
| مراجع | ||
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