Alteration in cardiac uncoupling proteins and eNOS gene expression following high-intensity interval training in favor of increasing mechanical efficiency | ||
| Iranian Journal of Basic Medical Sciences | ||
| مقاله 6، دوره 19، شماره 3، خرداد 2016، صفحه 258-264 اصل مقاله (1.01 M) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22038/ijbms.2016.6644 | ||
| نویسندگان | ||
| Ali Asghar Fallahi1؛ Shahnaz Shekarfroush* 2؛ Mostafa Rahimi3؛ Amirhossain Jalali4؛ Ali Khoshbaten5 | ||
| 1Faculty of Education and Psychology, Shiraz University, Shiraz, Iran | ||
| 2Department of Physiology, Arsanjan Branch, Islamic Azad University, Fars, Iran | ||
| 3Department of Physical Education, University of Kashan, Iran | ||
| 4Institute of Biotechnology and Bioengineering, Isfahan University of Technology, Isfahan, Iran | ||
| 5Sport Physiology Research Center, Baghiyatallah University of Medical Sciences, Tehran, Iran | ||
| چکیده | ||
| Objective(s):High-intensity interval training (HIIT) increases energy expenditure and mechanical energy efficiency. Although both uncoupling proteins (UCPs) and endothelial nitric oxide synthase (eNOS) affect the mechanical efficiency and antioxidant capacity, their effects are inverse. The aim of this study was to determine whether the alterations of cardiac UCP2, UCP3, and eNOS mRNA expression following HIIT are in favor of increased mechanical efficiency or decreased oxidative stress. Materials and Methods: Wistar rats were divided into five groups: control group (n=12), HIIT for an acute bout (AT1), short term HIIT for 3 and 5 sessions (ST3 and ST5), long-term training for 8 weeks (LT) (6 in each group). The rats of the training groups were made to run on a treadmill for 60 min in three stages: 6 min running for warm-up, 7 intervals of 7 min running on treadmill with a slope of 5° to 20° (4 min with an intensity of 80-110% VO2max and 3 min at 50-60% VO2max), and 5-min running for cool-down. The control group did not participate in any exercise program. Rats were sacrificed and the hearts were extracted to analyze the levels of UCP2, UCP3 and eNOS mRNA by RT-PCR. Results:UCP3 expression was increased significantly following an acute training bout. Repeated HIIT for 8 weeks resulted in a significant decrease in UCPs mRNA and a significant increase in eNOS expression in cardiac muscle. Conclusion:This study indicates that Long term HIIT through decreasing UCPs mRNA and increasing eNOS mRNA expression may enhance energy efficiency and physical performance. | ||
| کلیدواژهها | ||
| eNOS؛ High-intensity interval training؛ UCP2؛ UCP3 | ||
| مراجع | ||
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