Preventive effects of hydroalcoholic extract of saffron on hematological parameters of experimental asthmatic rats | ||
| Avicenna Journal of Phytomedicine | ||
| مقاله 10، دوره 3، شماره 3، شهریور 2013، صفحه 279-287 اصل مقاله (225.49 K) | ||
| نوع مقاله: Original Research Article | ||
| شناسه دیجیتال (DOI): 10.22038/ajp.2013.656 | ||
| نویسندگان | ||
| Somayyeh Vosooghi1؛ Maryam Mahmoudabady2؛ Ali Neamati* 3؛ Heydar Aghababa4 | ||
| 1Department of Biology, Payame Noor University, Boshruyeh, South Khorasan, I. R. Iran | ||
| 2Applied Physiology Research Centre and Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, I. R. Iran | ||
| 3Department of Biology, Faculty of Science, Mashhad Branch, Islamic Azad University, Mashhad, I. R. Iran | ||
| 4Department of Biology, Faculty of Science, Arsanjan Branch, Islamic Azad University, I. R. Iran | ||
| چکیده | ||
| Objective: Asthma is a chronic inflammatory disease of the respiratory airways distinguished by edema and infiltration of inflammatory immune cells. To test our hypothesis about the anti-inflammatory effect of saffron, we examined effects of Crocus sativus (C. sativus) extract as a prophylactic anti-inflammatory agent in sensitized rats. Materials and Methods: To induce experimental asthma, rats were sensitized with injection and inhalation of ovalbumin (OA). Forty male Wistar rats were divided into 5 groups (n=8 for each): control, sensitized (asthma), and sensitized and pretreated with three different concentrations of extract, 50, 100, and 200 mg/kg, 2 times a week (group asthma+50EX, group asthma+100EX, and group asthma+200EX). After 32 days, total white blood cells (WBC) counts, red blood cells (RBC), and platelet counts in blood were examined. Results: Total WBC number and eosinophil and neutrophil percentage in blood were increased, but lymphocyte decreased in sensitized animals compared with those of control group (pConclusion: Our findings indicated that the extract of C. sativus could be useful to prevent asthma as an anti-inflammatory treatment. | ||
| کلیدواژهها | ||
| Asthma؛ Crocus sativus؛ Inflammation؛ Rat؛ Sensitization؛ WBC | ||
| مراجع | ||
|
References
Adamko D, Odemuyiwa SO, Moqbel R. 2003. The eosinophil as a therapeutic target in asthma: beginning of the end, or end of the beginning? Curr Opin Pharmacol 3: 227-232.
Assimopoulou AN, Sinakos Z, Papageorgiou VP. 2005. Radical scavenging activity of Crocus sativus L. extract and its bioactive constituents. Phytother Res, 19: 997-1000.
Averill FJ, Hubbard WC, Proud D, Gleich GJ, Liu MC. 1992. Platelet activation in the lung after antigen challenge in a model of allergic asthma. Am Rev Respir Dis, 145: 571-576.
Barrett NA, Austen KF. 2009. Innate cells and T helper 2 cell immunity in airway inflammation. Immunity, 31: 425-437.
Bloemen K, Verstraelen S, Van Den Heuvel R, Witters H, Nelissen I, Schoeters G. 2007. The allergic cascade: review of the most important molecules in the asthmatic lung. Immunol Lett, 113: 6-18.
Boskabady MH, Adel-Kardan S. 1999. Increased muscarinic receptor blockade by atropine in tracheal chains of ovalbumin-sensitized guinea pigs. Pharmacology, 58: 300-308.
Boskabady MH, Aslani MR. 2006. Relaxant effect of Crocus sativus (saffron) on guinea-pig tracheal chains and its possible mechanisms. J Pharm Pharmacol, 58: 1385-1390.
Boskabady MH, Eslamizade MJ, Tabatabaei A, Nemati H, Mansouri F. 2010a. Effect of inhaled fluticasone on lung inflammation administered during and after guinea pig sensitization. Arch Bronconeumol, 46: 215-222.
Boskabady MH, Ghasemzadeh Rahbardar M, Nemati H, Esmaeilzadeh M. 2010b. Inhibitory effect of Crocus sativus (saffron) on histamine (H1) receptors of guinea pig tracheal chains. Pharmazie, 65, 300-305.
Boskabady MH, Tabatabaee A, Byrami G. 2012. The effect of the extract of Crocus sativus and its constituent safranal, on lung pathology and lung inflammation of ovalbumin sensitized guinea-pigs. Phytomedicine, 19: 904-911.
Boskabady MH, Ziaei T. 2003. Effect of ascorbic acid on airway responsiveness in ovalbumin sensitized guinea pigs. Respirology, 8: 473-478.
Flood-Page P, Menzies-Gow A, Phipps S, Ying S, Wangoo A, Ludwig MS, Barnes N, Robinson D, Kay AB. 2003. Anti-IL-5 treatment reduces deposition of ECM proteins in the bronchial subepithelial basement membrane of mild atopic asthmatics. J Clin Invest, 112: 1029-1036.
Gleich GJ. 2000. Mechanisms of eosinophil-associated inflammation. J Allergy Clin Immunol, 105: 651-663.
Hosseinzadeh H, Ghenaati J. 2006. Evaluation of the antitussive effect of stigma and petals of saffron (Crocus sativus) and its components, safranal and crocin in guinea pigs. Fitoterapia, 77: 446-448.
Hosseinzadeh H, Nassiri-Asl M. 2012. Avicenna's (Ibn Sina) the Canon of Medicine and Saffron (Crocus sativus): A Review. Phytother Res, 27:475-483.
Hosseinzadeh H, Younesi HM. 2002. Antinociceptive and anti-inflammatory effects of Crocus sativus L. stigma and petal extracts in mice. BMC Pharmacol, 2: 7.
Humbles AA, Lloyd CM, McMillan SJ, Friend DS, Xanthou G, McKenna EE, Ghiran S, Gerard NP, Yu C, Orkin SH, Gerard C. 2004. A critical role for eosinophils in allergic airways remodeling. Science, 305: 1776-1779.
Kim CK, Kim SW, Park CS, Kim BI, Kang H, Koh YY. 2003. Bronchoalveolar lavage cytokine profiles in acute asthma and acute bronchiolitis. J Allergy Clin Immunol, 112: 64-71.
Kowal K, Pampuch A, Kowal-Bielecka O, DuBuske LM, Bodzenta-Lukaszyk A. 2006. Platelet activation in allergic asthma patients during allergen challenge with Dermatophagoides pteronyssinus. Clin Exp Allergy, 36, 426-432.
Mousavi SZ, Bathaie SZ. 2011. Historical uses of saffron: Identifying potential new avenues for modern research Avicenna J Phytomed, 1: 57-66.
Nakagome K, Nagata M. 2011. Pathogenesis of airway inflammation in bronchial asthma. Auris Nasus Larynx, 38: 555-63.
Nemati H, Boskabady MH, Ahmadzadef Vostakolaei H. 2008. Stimulatory effect of Crocus sativus (saffron) on beta2-adrenoceptors of guinea pig tracheal chains. Phytomedicine, 15: 1038-1045.
Pearlman DS. 1999. Pathophysiology of the inflammatory response. J Allergy Clin Immunol, 104: S132-137.
Prussin C, Metcalfe DD. 2006. IgE, mast cells, basophils, and eosinophils. J Allergy Clin Immunol, 117: S450-456.
Rios JL, Recio MC, Giner RM, Manez S. 1996. An update review of saffron and its active constituents. Phytother Res 10, 189-193.
Tarantilis PA, Tsoupras G, Polissiou M. 1995. Determination of saffron (Crocus sativus L.) components in crude plant extract using high-performance liquid chromatography-UV-visible photodiode-array detection-mass spectrometry. J Chromatogr A, 699: 107-118.
| ||
|
آمار تعداد مشاهده مقاله: 32,252 تعداد دریافت فایل اصل مقاله: 6,478 |
||