Adriamycin-induced oxidative stress is prevented by mixed hydro-alcoholic extract of Nigella sativa and Curcuma longa in rat kidney | ||
| Avicenna Journal of Phytomedicine | ||
| مقاله 9، دوره 6، شماره 1، فروردین و اردیبهشت 2016، صفحه 86-94 اصل مقاله (493.2 K) | ||
| نوع مقاله: Original Research Article | ||
| شناسه دیجیتال (DOI): 10.22038/ajp.2016.5486 | ||
| نویسندگان | ||
| Reza Mohebbati1؛ Mohammad Naser Shafei2؛ Mohammad Soukhtanloo3؛ Noema Mohammadian Roshan4؛ Abolfazl Khajavi Rad* 5؛ Akbar Anaeigoudari6؛ Sara Hosseinian1؛ Sareh Karimi1؛ Farimah Beheshti1 | ||
| 1Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran | ||
| 2Neurocognitive Research Center and department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran | ||
| 3Department of Biochemistry, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran | ||
| 4Department of Pathology, Qaem Hospital, Mashhad University of Medical Sciences, Mashhad, Iran | ||
| 5Neurogenic Inflammation Research Center, Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran | ||
| 6Department of Physiology, School of Medicine, Jiroft University of Medical Sciences, Jiroft, Iran | ||
| چکیده | ||
| Objective: Inflammation and oxidative stress is considered to have a crucial role in induction of nephropathy. Curcuma longa (C. longa) and Nigella sativa (N. sativa) have anti-inflammatory and antioxidant effects. This study was designed to investigate the effect of mixed hydro-alcoholic extract of N.sativa and C. longa on the oxidative stress induced by Adriamycin (ADR) in rat kidney. Material and Method: The animals were divided into 6 groups: control (CO), ADR, Adriamycin+ Vitamin C (ADR+VIT C), C. longa extract+ Adriamycin (C.LE+ADR), N. sativa extract+ Adriamycin (N.SE+ADR) and C. longa extract+ N. sativa extract + Adriamycin (N.S+C.L+ADR). ADR (5mg/kg) was injected intravenously, whereas VITC (100mg/kg) and extract of C. longa (1000mg/kg) and N. sativa (200mg/kg) were administrated orally. Finally, the renal tissue, urine and blood samples were collected and submitted to measure of redox markers, osmolarity and renal index. Results: The renal content of total thiol and superoxide dismutase (SOD) activity significantly decreased and Malondialdehyde (MDA) concentration increased in Adriamycin group compared to control group. The renal content of total thiol and SOD activity significantly enhanced and MDA concentration reduced in treated-mixed extract of C. longa and N. sativa along with ADR group compared to ADR group. The mixed extract did not restore increased renal index percentage induced by ADR. There also was no significant difference in urine and serum osmolarity between the groups. Conclusion: hydro-alcoholic extracts of N.sativa and C.longa led to an improvement in ADR-induced oxidative stress and mixed administration of the extracts enhanced the aforementioned therapeutic effect. | ||
| کلیدواژهها | ||
| Adriamycin؛ Nigella Sativa؛ Curcuma longa؛ Oxidative stress | ||
| مراجع | ||
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