Hepatoprotective effect of Rosa canina fruit extract against carbon tetrachloride induced hepatotoxicity in rat | ||
| Avicenna Journal of Phytomedicine | ||
| مقاله 5، دوره 6، شماره 2، خرداد و تیر 2016، صفحه 181-188 اصل مقاله (892.3 K) | ||
| نوع مقاله: Original Research Article | ||
| شناسه دیجیتال (DOI): 10.22038/ajp.2016.5481 | ||
| نویسندگان | ||
| Heibatollah Sadeghi1؛ Abdol Saleh Hosseinzadeh2؛ Mehdi Akbartabar Touri3؛ Mehdi Ghavamzadeh2؛ Mehrzad Jafari Barmak4؛ Hossein Sadeghi* 5؛ Moslem sayahi2 | ||
| 1Medicinal Plants Research Center, Yasuj University of Medical Sciences, Yasuj, Iran | ||
| 2Student Research Committee, Yasuj University of Medical Sciences, Yasuj, Iran | ||
| 3Social Determinants of Health Research Center, Yasuj University of Medical Sciences, Yasuj, Iran | ||
| 4Cellular and Molecular Research Center,Yasuj University of Medical Sciences, Yasuj, Iran | ||
| 5Department of Pharmacology, Faculty of Medicine, Yasuj University of Medical Sciences, Yasuj, Iran | ||
| چکیده | ||
| Objective: The present study was conducted to investigate the hepatoprotective activity of hydro-ethanolic fruit extract of Rosa canina (R. canina) against carbon tetrachloride (CCl4)-induced hepatotoxicity in rats. Methods: Male Wistar albino rats were randomly divided into six groups of 8 animals of each, including control, toxic (CCl4), R. canina 250, 500, and 750 mg/kg + CCl4 and R. canina 750 mg/kg alone. R. canina (p.o., daily) and CCl4 (1 ml/kg twice a week, 50% v/v in olive oil, i.p.) were administered to animals for six weeks. Serum analysis was performed to assay the levels of aspartate aminotransferase (AST), alanine amino transaminase (ALT), alkaline phosphatase (ALP), albumin (ALB), total protein (TP) and malondialdehyde (MDA). Biochemical observations were also supplemented with histopathological examination (haematoxylin and eosin staining) of liver section. Results: Hepatotoxicity was evidenced by considerable increase in serum levels of AST, ALT, ALP, and lipid peroxidation (MDA) and decrease in levels of ALB and TP. Injection of CCL4 also induced congestion in central vein, and lymphocyte infiltration. Treatment with hydro-alcoholic fruit extract of R. canina at doses of 500 and 750 mg/kg significantly reduced CCl4-elevated levels of ALT, AST, ALP and MDA (p<01). The extract also increased the serum levels of ALB and TP compared to CCl4 group (p<01) at the indicated dose Histopathological studies supported the biochemical finding. Conclusion: our finding indicated hepatoprotective effects of the hydro-alcoholic fruit extract of R. canina on CCl4-induced hepatic damage in rats and suggested that theses effect may be produced through reducing oxidative stress. | ||
| کلیدواژهها | ||
| Hepatoprotective؛ Tetrachloride؛ Rosa canina fruit؛ Rats | ||
| مراجع | ||
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Akbartabar Toori M, Joodi B, Sadeghi H, Sadeghi H, Jafari M, Talebianpoor MS, Mehraban F, Mostafazadeh M, Ghavamizadeh M. 2015. Hepatoprotective activity of aerial parts of Otostegiapersica against carbon tetrachloride-induced liver damage in rats. Avicenna J Phytomed, 5:238-246.
Akindele AJ, Ezenwanebe KO, Anunobi CC, Adeyemi OO. 2010.Hepatoprotective and in vivo antioxidant effects of Byrsocarpus coccineus Schum. and Thonn. (Connaraceae), J Ethnopharmacol, 129: 46-52.
Amat N, Upur H, Blazeković B. 2010. In vivo hepatoprotective activity of the aqueous extract of Artemisia absinthium L. against chemically and immunologically induced liver injuries in mice. J Ethnopharmacol, 131: 478-484.
Mahmud Z, Bachar S, Qais N. 2012. Antioxidant and Hepatoprotective Activities of Ethanolic Extracts of Leaves of Premna esculenta Roxb. against Carbon Tetrachloride-Induced Liver Damage in Rats. J Young Pharm, 4: 228-234.
Buege, J. and Aust S.1978. The thiobarbuturic acid assay. Methods Enzymol, 52: 306-307.
Campos-Vega R, Loarca-Piña G, Oomah BD. 2010. Minor components of pulses and their potential impact on human health. Food Res Int, 43: 461-482.
Cho BO, Ryu HW, So Y, Jin CH, Baek JY, Park KH, Byun EH, Jeong IY. 2013. Hepatoprotective effect of 2,3-dehydrosilybin on carbon tetrachloride-induced liver injury in rats. Food Chem, 138: 107-115.
Chrubasik C, Roufogalis BD, Müller-Ladner U, Chrubasik S.2008. A systematic review on the Rosa canina effect and efficacy profiles. PhytotherRes, 22: 725-733.
Daels-Rakotoarison DA, Gressier B, Trotin F, Brunet C, Luyckx M, Dine T, Bailleul F, Cazin M, Cazin JC. 2002. Effects of Rosa canina fruit extract on neutrophil respiratory burst. Phytother Res, 16: 157-161.
Demir, F. and Özcan M. 2001. Chemical and technological properties of rose (Rosa canina L.) fruits grown wild in Turkey. J Food Eng, 47: 333-336.
Deliorman Orhan D, Hartevioğlu A, Küpeli E, Yesilada E. 2007. In vivo anti-inflammatory and antinociceptive activity of the crude extract and fractions from Rosa canina L. fruits.J Ethnopharmacol, 112:394-400.
Fattahi, S, Jamei, R, Hosseini Sarghein S. 2012. Antioxidant and antiradical activities of Rosa canina and Rosa pimpinellifolia fruits from West Azerbaijan. IJPP, 2: 523-529.
Fecka, I. 2009. Qualitative and quantitative determination of hydrolysable tannins and other polyphenols in herbal products from meadowsweet and dog rose. Phytochem Anal, 20: 177-190.
GhiorghitaG, MaftelDE, Nicuta D, RatiI V.2012. Study of several parameters in Rosa canina L. genotypes from native habitats in Romania and the in vitro response of this species. Acad Roman Sci Ann - Series Biol Sci, 1:91-109.
Huang GJ, Deng JS, Huang SS, Lee CY, Hou WC, Wang SY, Sung PJ, Kuo YH. 2013. Hepatoprotective effects of eburicoic acid and dehydroeburicoic acid from Antrodia camphorata in a mouse model of acute hepatic injury. Food Chem, 141: 3020-3027.
Hvattum E. 2002. Determination of phenolic compounds in rose hip (Rosa canina) using liquid chromatography coupled to electrospray ionisation tandem mass spectrometry and diode‐array detection. RCM, 16: 655-662.
Jaishree V and Badami S. 2010.Antioxidant and hepatoprotective effect of swertiamarin from Enicostemma axillare against d-galactosamine induced acute liver damage in rats. J Ethnopharmacol, 130: 103-106.
Kale I, Asif Khan M, Irfan Y, Veerana GA.2012. Hepatoprotective potential of ethanolic and aqueous extract of flowers of Sesbania grandiflora (Linn) induced by CCl4. Asian Pac J Trop Biomed, 2: S670-S679.
Kepekçi RA, Polat S, Çelik A, Bayat N, Saygideger SD. 2013. Protective effect of Spirulina platensis enriched in phenolic compounds against hepatotoxicity induced by CCl4. Food Chem, 141: 1972-1979.
Kilicgun H, and Altiner D. 2010. Correlation between antioxidant effect mechanisms and polyphenol content of Rosa canina. Pharmacogn Mag, 6: 238.
Lattanzio F, Greco E, Carretta D, Cervellati R, Govoni P, Speroni E. 2011. In vivo anti-inflammatory effect of Rosa canina L. extract. J Ethnopharmacol, 137: 880-885.
M, Siddiqui HH, Hussain A, Hussain MS .2013. Hepatoprotective effects of Adenanthera pavonina (Linn.) against anti-tubercular drugs-induced hepatotoxicity in rats. Phcog J, 5: 286-290.
Murat Bilgin H, Atmaca M, Deniz Obay B, Ozekinci S, Taşdemir E, Ketani A. 2011. Protective effects of coumarin and coumarin derivatives against carbon tetrachloride-induced acute hepatotoxicity in rats. Exp Toxicol Pathol, 63: 325-330.
Montazeri N, BaherE, Mirzajani F, Barami Z,YousefianS. 2011. Phytochemical contents and biological activities of Rosa Canina fruit from Iran. J Med Plant Res, 5: 4584-4589.
Deliorman Orhan D, Hartevioğlu A, Küpeli E, Yesilada E. 2007. In vivo anti-inflammatory and antinociceptive activity of the crude extract and fractions from Rosa canina L. fruits. J Ethnopharmacol, 112: 394-400.
Ottu OJ,Atawodi SE, Onyike E.2013. Antioxidant, hepatoprotective and hypolipidemic effects of methanolic root extract of Cassia singueana in rats following acute and chronic carbon tetrachloride intoxication. Asian Pac J Trop Med, 6: 609-615.
Pareek A, Godavarthi A, Issarani R, Nagori BP. 2013. Antioxidant and hepatoprotective activity of Fagonia schweinfurthii (Hadidi) Hadidi extract in carbon tetrachloride induced hepatotoxicity in HepG2 cell line and rats. J Ethnopharmacol, 150: 973-981.
Ravikumar S and Gnanadesigan M. 2012. Hepatoprotective and Antioxidant Properties of Rhizophora mucronata Mangrove Plant in CCl4 Intoxicated Rats. J Exp Clin Med, 4: 66-72.
Roman I, Stănilă A, Stănilă S. 2013. Bioactive compounds and antioxidant activity of Rosa canina L. biotypes from spontaneous flora of Transylvania. Chem Cent J, 7: 73.
Sadeghi H, Zarezade V, Sadeghi H, Akbartabar Toori M, Jafari Barmak M, Azizi A, Ghavamizadeh M, Mostafazadeh M. 2014. Anti-inflammatory Activity of Stachys Pilifera Benth. Iran Red Crescent Med J, 16:9.
Sathesh Kumar S1, Ravi Kumar B, Krishna Mohan G. 2009. Hepatoprotective effect of Trichosanthes cucumerina Var cucumerina L. on carbon tetrachloride induced liver damage in rats. J Ethnopharmacol, 123: 347-350.
Serteser A, Kargioğlu M, Gök V, Bağci Y, Ozcan MM, Arslan D.2009. Antioxidant properties of some plants growing wild in Turkey. Int J Food Sci Nutr, 60: 147-154.
Sharma JB, Sharma A, Bahadur A, Vimala N, Satyam A, Mittal S. 2006. Oxidative stress markers and antioxidant levels in normal pregnancy and pre-eclampsia. Int J Gynaecol Obstet, 94: 23-27.
Srivastava A and Shivanandappa T. 2006. Hepatoprotective effect of the aqueous extract of the roots of Decalepis hamiltonii against ethanol-induced oxidative stress in rats. Hepatol Res, 35: 267-275.
Swarnalatha L and Reddy P.N. 2012. Hepatoprotective activity of Sphaeranthus amaranthoides on D–galactosamine induced hepatitis in albino rats. Asian Pac J Trop Biomed 2: S1900-S1905.
Wenzig EM, Widowitz U, Kunert O, Chrubasik S, Bucar F, Knauder E, Bauer R. 2008. Phytochemical composition and in vitro pharmacological activity of two rose hip (Rosa canina L.) preparations. Phytomedicine, 15: 826-835.
Wu Y, Yang L, Wang F, Wu X, Zhou C, Shi S, Mo J, Zhao Y. 2007. Hepatoprotective and antioxidative effects of total phenolics from Laggera pterodonta on chemical-induced injury in primary cultured neonatal rat hepatocytes. Food Chem Toxicol, 45: 1349-1355.
Yang L, Wang CZ, Ye JZ, Li HT. 2011. Hepatoprotective effects of polyprenols from Ginkgo biloba L. leaves on CCl4-induced hepatotoxicity in rats. Fitoterapia, 82: 834-840.
Zdarilová A, Svobodová A, Simánek V, Ulrichová J. 2009. Prunella vulgaris extract and rosmarinic acid suppress lipopolysaccharide-induced alteration in human gingival fibroblasts. Toxicol In Vitro, 23: 386-392.
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