Detection of Pseudomonas and Flavobacterium Species Harbouring Organophosphorus Degrading Elements from Environment | ||
| Iranian Journal of Basic Medical Sciences | ||
| مقاله 6، دوره 10، شماره 4، دی 2008، صفحه 239-244 اصل مقاله (859.96 K) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22038/ijbms.2008.5237 | ||
| نویسندگان | ||
| Aboalfazl Nazarian* 1؛ Bahram Amini2 | ||
| 1Department of Clinical Biochemistry, Faculty of medicine, Zanjan University of Medical Sciences, Zanjan, Iran | ||
| 2Department of Clinical Microbiology, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran | ||
| چکیده | ||
| Objective The extensive pollution of natural ecosystems has occurred by organophosphorus pesticides, being used for improvement of crop production in agriculture. Biodegradation is interested in application of multifunctional bacteria containing genetical elements which specially degrade organophosphorus compounds. In this paper the detection of two bacteria species, Pseudomonas and Flavobacterium, which harbouring organophosphorus degrading elements from environmental samples is reported. Materials and Methods Several resistant strains isolated from different cultures that previously were cultivated with related sources such as soils and waters. These bacteria had been isolated from organophosphorus enriched mineral solutions, and following inoculation, visible colonies were observed on mineral and MacConkey’s agar medium. The resistant bacteria were identified by conventional procedures and monitored with certain properties like stability, tolerance, resistance to organophosphorus compounds and different antibiotics. The genetical elements for organophosphate degradation were confirmed by adding acridine orange to culture of resistant strains which could delete these capabilities. Results Fifty strains were optimally grown in presence of three classes of organophophoruses like guthion, dimethoate and methyl parathion with concentrations of 2.5, 4, and 8 gl-1 respectively. The gram-negative bacteria were identified using conventional diagnostic procedures, as Pseudomonas and Flavobacterium species. These strains were harbouring organophosphorus degrading elements which were deleted by acridine orange as mutagen. Also, most of these bacteria were resistant to different antibiotics that used as biomarkers in discrimination of sensitive strains to organophosphoruses. Conclusion The genetical elements such as resistance to organophosphoruses and antibiotics were transferred to the sensitive bacteria by matting technique and cured as multifunctional organisms which had high capability for various organophosphoruses. The multi-degrading strains might be suggested as useful tools for detoxification of harmful organophosphoruses in agriculture. | ||
| کلیدواژهها | ||
| Biodegradation؛ Catalytic enzymes؛ Organophosphorus degrading elements؛ Resistant bacteria | ||
| مراجع | ||
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1. Mathur S C. The pesticides industry in India .In chemical weekly Annual Ed Raghavan R:.NewDelhi: Pesticide Association of India publication; 1992.185-192.
2. Al-Jughbir MT, Salhab AF, Hamarsheh F A. Dermal & Inhalation exhibition to dimethoate. Archives Environment Contam Toxicol 1992; 22:358-361.
3. Nazarian A. Utilization of Dimethoate by Wild Type Pseudomonas Putida from polluted Sites in Iran. Pak J Bio Sci 2007; 10(4): 664-667.
4. Munnecke DM. Hydrolysis of organophosphate insecticides by an immobilized-enzyme system. Biotechnol Bioenginee 1979; 21(12): 2247-2261.
5. Anonymous, Environmental health criteria 63, Organophosphorus Insecticides. WHO, Geneva, 2004. ISBN 9241542632.
6. Deshpande N M, Kanekar PP. Plasmid – mediated degradation in Pseudomonas aeroginosa MCMB-427. Letters in Applied Microbiology 2001; 33:275-279.
7. Hayatsu M,Hirano M,Tokuda S.Involvement of two Plasmids in Fenitrothion degradation by Burkhoderia sp strain NF100.Appl Environ Microbiol 2000; 54:288-293.
8. Finegold S M,Martin WJ. Diagnostic Microbiology, 6th Ed. London: Mosby; 1982. 249-279.
9. Ying Yong Sheng Tai Xne Bao. Isolation and determination of silver resistant bacterial plasmids 2007; 17(2):305-8. Article in Chinese. PMID: 16706059 [Pud Med – indexed for MEDLINE].
10. Spengler G, Molnar A, Schelz Z, Amaral L, Sharples D Monlar J. The mechanism of plasmid wring in bacteria. Curr. Drug Targets 2006; 7 (7): 823 – 41.
11. Bertanie I, Kojic M, Venturi V. Regulation of the p-hydroxybenzoic acid hydroxylase gene (pobA) in plant-growth-promoting Pseudomonas Putida WCS358. Microbiology 2001; 147: 1611-1620.
12. Chen Y,.Zhang X, Liu H, Wang Y, Xia X. Study on Pseudomonas sp. WBC-3 capable of complete degradation of methylparathion. Wei Sheng Wu Xue Bao, 2002; 42(4):490-7.
13. Van der Meer JR, De Vos WM ,Harayama S , Zehnder A J. Molecular mechanisms of genetic adaptation to xenobiotic compounds. Microbiol Rev 1992; 56(4):677-694.
14. Morales GJF, Linares A, Beloso JP, Albar JL, Martinez, Rojo F. The Pseudomonas Putida Crc. Global Regulator Controls the Expression of Genes from Several Chromosomal Catabolic Pathways for Aromatic Compounds. J Bacteriol 2004; 186(5): 1337 – 1344.
15. Chauddhry GR, Ali AN,Wheeler WB. Isolation of a methyl parathion-degrading Pseudomonas sp. That possesses DNA homologous to the opd gene from a Flavobacterium sp. Appl Environ Microbiol 1988; 54(2): 288-93.
16. Aislabie J G. Lloyd-Jones .A review of bacterial degradation of pesticides. Australian Journal of Soil Research 1995; 33(6): 925-942.
17. Eduardo D, Ferrandez A, Prieto M A, Garcia J L. Biodegradation of Aromatic Compounds by Escherichia coli. Microbiol Molecular Biol Reviews 2001; 65(4): 523-569.
18. Lewinson O, Bibi E.Evidence for simultaneous binding of dissimilar substrates by the Escherichia coli multidrug transporter MdfA. Biochemist 2001; 40(42):12612-8.
19. Lewinson O. The Escherichia coli multidrug transporter MdfA catalyzes both electrogenic and electroneutral transport reactions. Proc Natl Acad Sci USA 2003; 100(4):1667-72.
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