Detection of TEM, SHV and PER Type Extended-Spectrum ß-Lactamase Genes among Clinical Strains of Pseudomonas aeruginosa Isolated from Burnt Patients at Shafa-Hospital, Kerman, Iran | ||
| Iranian Journal of Basic Medical Sciences | ||
| مقاله 8، دوره 11، شماره 2، تیر 2008، صفحه 104-111 اصل مقاله (1.29 M) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22038/ijbms.2008.5220 | ||
| نویسندگان | ||
| Mohammad Reza Shakibaie* 1؛ Fereshteh Shahcheraghi2؛ Ali Hashemi1؛ N Saeed Adeli1 | ||
| 1Department of Mirobiology, Kerman University of Medical Sciences, Kerman, Iran | ||
| 2Department of Bacteriology, Institute Pasteur of Iran, Tehran, Iran | ||
| چکیده | ||
| Objective(s) This study was carried out to evaluate the existence of the TEM, SHV and PER ESBL genes in ESBL producing strains of Pseudomonas aeruginosa isolated from burnt patients at Shafa-hospital, Kerman, Iran. Materials and Methods A total of 120 strains of P. aeruginosa were isolated from 245 patients in burn unit of Shafa-hospital during January 2006 to December 2007. MIC of antibiotics was measured using agar dilution test. ESBL producing strains were detected by double-disc synergy method containing amoxicillin and amoxicillin+clavulanic acid and phenotypic confirmatory test. All the clinical isolates resistant to imipenem (IMP) were screened for the production of MBL by E-test with IMP/IMP+EDTA strips. PCR and multiplex-PCR performed for the detection of different types of ESBL producing genes in ESBL positive isolates. Results Of 120 the isolates, 3-5% showed MIC greater than 16 µg/ml to IMP and meropenem, 66% showed MIC greater than 32 µg/ml to ceftazidime, 42% to azteronam and 60% of the isolates showed MIC greater than 64 µg/ml to cefotaxime, 41 (34%) confirmed as ESBL producers. Not any isolate could produce MBL (P≤0.05). The PCR assay of all ESBL producing isolates revealed that 6.6%, 4.1% and 2.5% of them were positive for SHV, PER and TEM genes, respectively. Conclusion Many ESBL producing strains of P. aeruginosa isolated from patients in burn unit of Shafa-hospital. However, none could produce MBL enzyme. The genes among ESBL producing strains were SHV, PER as well as TEM type of β-lacatamases. | ||
| کلیدواژهها | ||
| Antibiotic resistance؛ ESBL؛ MBL؛ Pseudomonas aeruginosa | ||
| مراجع | ||
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