Effect of Shark Liver Oil on Peritoneal Murine Macrophages in Responses to Killed-Candida albicans | ||
| Iranian Journal of Basic Medical Sciences | ||
| مقاله 9، دوره 12، شماره 3، مهر 2009، صفحه 179-183 اصل مقاله (100.5 K) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22038/ijbms.2009.5180 | ||
| نویسندگان | ||
| Monire Hajimoradi* 1؛ Saeed Daneshmandi1؛ Zuhair Mohammad Hassan1؛ Maryam Roudbary2 | ||
| 1Department of Immunology, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran | ||
| 2Department of Mycology, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran | ||
| چکیده | ||
| Objective(s) Shark Liver Oil (SLO) is an immunomodulator. Macrophages play a key role in host defense against pathogens like fungi. Candida albicans have mechanisms to escape immune system. We determined the effect of killed-Candida on the in vitro viability of macrophages and the effect of SLO on augmentation of this potency. Materials and Methods Peritoneal macrophages were separated and cultured (3*105/well). At first, the effect of killed-Candida (200 cells/well) on macrophage viability was evaluated, using MTT test. Then, MTT was performed on macrophages stimulated with killed-Candida in the presence of SLO. Results Killed-Candida suppressed the ability of MTT reduction and hence macrophages viability (P=0.026), but addition of SLO (100 mg/ml) significantly enhanced cell viability (P=0.00). So, SLO could neutralize the inhibitory effect of Candida. Conclusion Simultaneous with cytotoxic effect of killed-Candida cells on macrophages viability, SLO augment macrophages viability. So, it can be applied in candidiasis as a complement. | ||
| کلیدواژهها | ||
| Candida albicans؛ Macrophage؛ Shark؛ Liver Oil | ||
| مراجع | ||
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