A Functional Polymorphism of the Granulocyte Macrophage Colony Stimulating Factor is not Associated with the Outcome of HTLV-I Infection | ||
| Iranian Journal of Basic Medical Sciences | ||
| مقاله 7، دوره 13، شماره 2، تیر 2010، صفحه 40-47 اصل مقاله (253.63 K) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22038/ijbms.2010.5082 | ||
| نویسندگان | ||
| Abbas Shirdel* 1؛ Houshang Rafatpanah2، 3؛ Hassan Rahimi1؛ Abdol Rahim Rezaee2؛ Mahmoud Reza Azarpajooh4؛ Akram Beyk yazdi1؛ Ian V Hutchinson5 | ||
| 1Internal Medicine Department, Ghaem Hospital, Mashhad University of Medical Sciences, Mashhad, Iran | ||
| 2Immunology Research Centre, BuAli Reserch Institute, Mashhad University of Medical Sciences, Mashhad, Iran | ||
| 3Centre for Integrated Genomic Medical Research (CIGMR), the University of Manchester, Manchester, UK | ||
| 4Neurology Department, Ghaem Hospital, Mashhad University of Medical Sciences, Mashhad, Iran | ||
| 5Immunology Research Group, Faculty of Life Sciences, the University of Manchester, UK | ||
| چکیده | ||
| Introduction Genetic background has known to be associated with the outcome of human T cell lymphotropic virus (HTLV) type I infection. In The present study we investigate the association between GM-CSF gene polymorphisms with the outcome of HTLV-I infection. Materials and Methods We analyzed 3 single-nucleotide polymorphisms in the promter region of granulocyte macrophage colony stimulating factor (GM-CSF) at positions -677*A/C, -1440*A/G and -1916*T/C in 68 patients with HTLV- I-associated myelopathy/tropical spastic paraparesis (HAM/TSP) and 77 HTLV-I-seropositive asymptomatic carriers and 175 healthy controls from an area in Iran, Mashhad, where HTLV-I is endemic. Results No significant differences were observed in the distribution of GM-CSF polymorphisms between HAM/TSP patients, HTLV-I carriers and healthy controls (P> 0.05). The -677*A/C polymorphism fall within the transcriptional enhancer factor-2 (TEF-2) binding site, so an electrophoretic mobility shift assay (EMSA) was performed to determine the effects of polymorphisms on protein binding to the GM-CSF promoter. The result showed a significantly higher binding efficiency of nuclear protein to the A allele compared with the C allele. Conclusion Our study suggests that polymorphisms in the GM-CSF promoter is not associated with the outcome of HTLV-I infection, however, GM-CSF polymorphism at position -677 could indeed influence gene expression. | ||
| کلیدواژهها | ||
| Electrophoretic Mobility Shift Assay؛ Gene Polymorphisms؛ Granulocyte-Macrophage Colony-Stimulating Factor؛ Human T-lymphotropic virus 1 | ||
| مراجع | ||
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