Combination of Stem Cell Mobilized by Granulocyte-Colony Stimulating Factor and Human Umbilical Cord Matrix Stem Cell: Therapy of Traumatic Brain Injury in Rats | ||
| Iranian Journal of Basic Medical Sciences | ||
| مقاله 4، دوره 14، شماره 4، مهر 2011، صفحه 327-339 اصل مقاله (624.65 K) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22038/ijbms.2011.5021 | ||
| نویسندگان | ||
| Mehrdad Bakhtiary1، 2؛ Mohsen Marzban* 1، 2؛ Mehdi Mehdizadeh1، 2؛ Mohammad Taghi Joghataei1، 2؛ Samideh Khoei1، 3؛ Mahdi Tondar2؛ Vahid Pirhajati Mahabadi2؛ Bahareh Laribi4؛ Asghar Ebrahimi5؛ Seyed Jafar Hashemian2؛ Navid Modiry2؛ Soraya Mehrabi6 | ||
| 1Cellular & Molecular Research Center, Tehran University of Medical Sciences, Tehran, Iran | ||
| 2Department of Anatomy, Tehran University of Medical Science, Tehran, Iran | ||
| 3Department of Medical Physics, Tehran University of Medical Sciences, Tehran, Iran | ||
| 4Department of Immunology, Tehran University of Medical Sciences, Tehran, Iran | ||
| 5Department of Biotechnology and Bioscience, Milano-Bicocca University, Milan, Italy | ||
| 6Department of physiology, Tehran University of Medical Sciences, Tehran, Iran | ||
| چکیده | ||
| Objective(s) Clinical studies of treating traumatic brain injury (TBI) with autologous adult stem cells led us to examine the impression of a combination therapy. This was performed by intravenous injection of human umbilical cord matrix stem cell (hUCMSC-Wharton,s jelly stem cell) with bone marrow cell mobilized by granulocytecolony stimulating factor (G-CSF) in rats injured with cortical compact device. Materials and Methods Adult male Wistar rats (n= 50) were injured with controlled cortical impact device and divided into five groups. All injections were performed 1 day after injury into the tail veins of rats. Neurological functional evaluation of animals was performed before and after injury using modified neurological severity scores (mNSS). Animals were sacrificed 42 days after TBI and brain sections were stained by Brdu immunohistochemistry. Results Statistically significant improvement in functional outcome was observed in treatment groups when compared with control (P< 0.01). mNSS showed no significant differences among the hUCMSC and G-CSF treated groups at any time point (end of trial). Rats with hUCMSC + G-CSF treatment had a significant improvement on mNSS at 5 and 6 week compared to other treatment group (P< 0.01). Conclusion Histological analysis in G-CSF+ hUCMSC treated traumatic rats exhibited significant increase in numbers of Brdu immunoreactive cells in their traumatic core compared with other labeled group. | ||
| کلیدواژهها | ||
| Combine Therapy؛ G-CSF؛ Stem cell؛ TBI؛ Whartons jelly | ||
| مراجع | ||
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