Protective Effect of Diosgenin and Exercise Training on Biochemical and ECG Alteration in Isoproterenol- Induced Myocardial Infarction in Rats | ||
| Iranian Journal of Basic Medical Sciences | ||
| مقاله 10، دوره 14، شماره 3، مرداد 2011، صفحه 264-274 اصل مقاله (147.07 K) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22038/ijbms.2011.5004 | ||
| نویسندگان | ||
| Afshin Salimeh؛ Mustafa Mohammadi* ؛ Gisou Mohaddes؛ Reza Badalzadeh | ||
| Department of Physiology, Drug Applied Research Center, Tabriz University of Medical Sciences,Tabriz, Iran | ||
| چکیده | ||
| Objective(s) Several studies have reported improved response of exercised hearts to myocardial infarction (MI). This study was aimed to evaluate the preventive role of treadmill exercise and diosgenin on cardiac marker enzymes, thiobarbituric acid reactive substances (TBARS), total antioxidant status (TAS), lipids, and electrocardiographic (ECG) patterns in isoproterenol (ISO)-induced myocardial infarction (MI) in male Wistar rats. Materials and Methods One hundred Wistar rats were divided into ten groups: Control rats (C), saline (S), L-cremephor (LC), exercise (E), diosgenin dissolved in L-cremephor (15 mg/kg/day) (D), exercise+ diosgenin (E+D), ISO injected (150 mg/kg) (ISO), exercise + ISO (E+ISO), diosgenin + ISO (D+ISO) and exercise+ diosgenin+ ISO (E+D+ISO). At the end of the experiment all animals anesthetized and blood samples were collected for biochemical estimation and also the ECG patterns were recorded. Results Exercise and diosgenin pretreatment significantly decreased the lactate dehydrogenase (LDH) and TBARS level in ISO injected animals. Exercise and diosgenin pretreatment significantly decreased serum total cholesterol and increased high density lipoprotein (HDL-C). ISO-treated rats showed pathological Q waves along with elevated ST segments. The altered electrocardiograms (ECG) of ISO-treated rats were also restored to near normal by diosgenin and exercise, but exercise and diosgenin had synergistic effects. Conclusion The present investigation demonstrates that combination of diosgenin and exercise exhibited significant protection against ISO induced electrocardiographical and biochemical changes. The cadioprotective mechanism(s) appear to be through changing lipid metabolism. | ||
| کلیدواژهها | ||
| Diosgenin؛ Isoproterenol؛ Myocardial infarction؛ Treadmill | ||
| مراجع | ||
|
1. Grundy SM. Cholesterol and coronary heart disease. A new era. JAMA 2004; 256:2849-2858.
2. Carlson LA, Böttiger LE. Risk factors for ischaemic heart disease in men and women. Results of the 19- year follow-up of the Stockholm Prospective Study. Acta Med Scand 1985; 218; 207-211.
3. Sushamakumari S, Jayadeep A, Kumar JS, Menon VP. Effect of carnitine on malondialdehyde, taurine and glutathione levels in heart of rats subjected to myocardial stress by isoproterenol. Indian J Exp Biol 1989; 27: 134-7.
4. Ithayarasi AP, Devi CS. Effect of alpha-tocopherol on lipid peroxidation in isoproterenol induced myocardial infarction in rats. Indian J Physiol Pharmacol 1997; 41:369-376.
5. Yeager JC, Iams SG.The hemodynamics of isoproterenol-induced cardiac failure in rats. Circ Shock 1981; 8:151–163.
6. Bloom S, Davis DL. Calcium as a mediator of isoproterenol-induced myocardial necrosis. Am J Pathol 1972; 69:459–470.
7. Singal PK, Kapur N, Dhillon KS, Beamis RE, Dhalla NS. Role of free radicals in catecholamine-induced cardiomyopathy. Can J Physiol Pharmacol 1983; 60:1390-1397.
8. Manjula TS, Devi CS. Effect of aspirin on isoproterenol induced changes in lipid metabolism in rats. Indian J Med Res 1993; 98:30-33.
9. Au AL, Kwok CC, Lee AT, Kwan YW, Lee MM, Zhang RZ, et al. Activation of iberiotoxin-sensitive, Ca2+- activated K+ channels of porcine isolated left anterior descending coronary artery by diosgenin. Eur J Pharmacol 2004; 502:123-33.
10. Son IS, Kim JH, Sohn HY, Son KH, Kim JS, Kwon CS. Antioxidative and hypolipidemic effects of diosgenin, a steroidal saponin of yam (Dioscorea spp.), on high-cholesterol fed rats. Biosci Biotechnol Biochem 2007; 71:3063-3071.
11. Yamashita N, Baxter GF, Yellon DM.Exercise directly enhances myocardial tolerance to ischaemia-reperfusion injury in the rat through a protein kinase C mediated mechanism. Heart. 2001; 85:331-336.
12. Brown DA, Chicco AJ, Jew KN, Johnson MS, Lynch JM, Watson PA, et al. Cardioprotection afforded by chronic exercise is mediated by the sarcolemmal, and not the mitochondrial, isoform of the KATP channel in the rat. J Physiol 2005; 569:913-924.
13. Darrah MI, Engen RL. Beneficial effects of exercise on l-isoproterenol induced myocardial infarction in male rats. Med Sci Sports Exerc 1982; 14:76-80.
14. Jen CJ, Liu YF, Chen HI.Short-term exercise training improves vascular function in hypercholesterolemic rabbit femoral artery. Chin J Physiol 2005; 48:79-85.
15. Yang AL,Chen HI. Chronic exercise reduces adhesion molecules/iNOS expression and partially reverses vascular responsiveness in hypercholesterolemic rabbit aortae. Atherosclerosis 2003; 169:11-7.
16. Weise SD, Grandjean PW, Rohack JJ, Womack JW, Crouse SF. Acute changes in blood lipids and enzymes in postmenopausal women after exercise. J Appl Physiol 2005; 99:609-15.
17. Kim KS, Paik IY, Woo JH, Kang BY. The effect of training type on oxidative DNA damage and antioxidant capacity during three-dimensional space exercise. Med Princ Pract 2010; 19:133-41.
18. Aubertin-Leheudre M, Lord C, Khalil A, Dionne IJ. Effect of 6 months of exercise and isoflavone supplementation on clinical cardiovascular risk factors in obese postmenopausal women: a randomized, double- blind study. Menopause 2007;14(4):624-9
19. Matsuda H, Pongpiriyadacha Y, Morikawa T, Kishi A, Kataoka S, Yoshikawa M. Protective effects of steroid saponins from Paris polyphylla var. yunnanensis on ethanol- or indomethacin-induced gastric mucosal lesions in rats: structural requirement for activity and mode of action. Bioorg Med Chem Lett 2003; 13:1101-1106.
20. Hayward R, Ruangthai R, Schneider CM, Hyslop RM, Strange R, Westerlind KC. Training enhances vascular relaxation after chemotherapy-induced vasoconstriction. Med Sci Sports Exerc 2004; 36:428-434.
21. Rajadurai M, Stanely P. Preventive effect of naringin on cardiac markers, electrocardiographic patterns and lysosomal hydrolases in normal and isoproterenol-induced myocardial infarction in Wistar rats. Toxicology 2007; 230:178-188.
22. Rothermel B, Vega RB, Yang J, Wu H, Bassel-Duby R, Williams RS. A protein encoded within the Down syndrome critical region is enriched in striated muscles and inhibits calcineurin signaling. J Biol Chem 2000; 275:8719–8725.
23- Drapr HH, Hadley M. Malondialdehyde determination as index of lipid peroxidation. Methods Enzymol 1990;186:421-31
24. Miller NJ, Rice-Evans C, Davies MJ, Gopinathan V, Milner A. A novel method for measuring antioxidant capacity and itsapplication to monitoring the antioxidant status in premature neonates. Clin Sci 1993; 84:407- 412.
25. Mathew S, Menon PV, Kurup PA. Effect of administration of vitamin A, ascorbic acid and nicotinamide adenine dinucleotide + flavin adenine dinucleotide on severity of myocardial infarction induced by isoproterenol in rats. Indian J Exp Biol 1985; 23:500-504.
26. Sumitra M, Manikandan P, Kumar DA, Arutselvan N, Balakrishna, K, Manohar BM, et al. Experimental myocardial necrosis in rats: role of arjunolic acid on platelet aggregation, coagulation and antioxidant status. Mol Cell Biochem 2001; 224:135-42.
27. Venditti P, Di Meo S. Antioxidants, tissue damage, and endurance in trained and untrained young male rats. Arch Biochem Biophys 1996; 31:63–68.
28. Radák Z, Sasvári M, Nyakas C, Pucsok J, Nakamoto H, Goto S. Exercise preconditioning against hydrogen peroxide-induced oxidative damage in proteins of rat myocardium. Arch Biochem Biophys 2000; 376: 248–251.
29. Radák Z, Kaneko T, Tahara S, Nakamoto H, Ohno H, Sasvári M, et al. The effect of exercise training on oxidative damage of lipids, proteins, and DNA in rat skeletal muscle: evidence for beneficial outcomes. Free Radic Biol Med 1999; 27:69–74.
30. Navarro A, Gomez C, López-Cepero JM, Boveris A. Beneficial effects of moderate exercise on mice aging: survival, behavior, oxidative stress, and mitochondrial electron transfer. Am J Physiol Regul Integr Comp Physiol 2004; 286:505–511.
31.Chan YC, Hsu CK,Wang MF,Su TY. A diet containing yam reduces the cognitive deterioration and brain lipid peroxidation in mice with senescence accelerated. Int J Food Sci Technol 2004; 39: 99-107.
32. Araghiniknam M, Chung S, Nelson-White T, Eskelson C, Watson RR. Antioxidant activity of dioscorea and dehydroepiandrosterone (DHEA) in older humans. Life Sci. 1996; 59: 147-57.
33. McAnuf MA, Omoruyi FO, Morrison EY, Asemota HN. Changes in some liver enzymes in streptozotocin- induced diabetic rats fed sapogenin extract from bitter yam (Dioscorea polygonoides) or commercial diosgenin. West Indian Med J 2005; 4:97-101.
34. Siu PM, Donley DA, Bryner RW, Always SE. Myogenin and oxidative enzyme gene expression levels are elevated in rat soleus muscles after endurance training. J Appl Physiol 2004; 97:277-285
35. Taylor RP, Ciccolo JT, Starnes JW. Effect of exercise training on the ability of the rat heart to tolerate hydrogen peroxide. Cardiovasc Res 2003; 58:575-581.
36. Su QS, Tian Y, Zhang JG, Zhang H. Effects of allicin supplementation on plasma markers of exercise-induced muscle damage, IL-6 and antioxidant capacity. Eur J Appl Physiol 2008; 103:275-283.
37. Alcerczyk A, Bartosz G. Thiols are main determinants of total antioxidant capacity of cellular homogenates. Free Radic Res 2003; 37:537-541.
38. Iborra RT, Ribeiro IC, Neves MQ, Charf AM, Lottenberg SA, Negrão CE, et al. Aerobic exercise training improves the role of high-density lipoprotein antioxidant and reduces plasma lipid peroxidation in type 2 diabetes mellitus. Scand J Med Sci Sports 2008; 18:742-750.
39. González D, Marquina R, Rondón N, Rodriguez-Malaver AJ, Reyes R. Effects of aerobic exercise on uric acid, total antioxidant activity, oxidative stress, and nitric oxide in human saliva. Res Sports Med 2008; 16:128-137.
40. Paritha IA, Devi CS. Effect of alpha-tocopherol on isoproterenol-induced changes in lipid and lipoprotein profile in rats. Indian J Pharmacol 1997; 29:399.
41. Leroith D. Dyslipidemia and glucose dysregulation in overweight and obese patients. Clin Cornerstone 2007; 8:38-52.
42. Laguna J, Gomez A, Pena A, Guzman-Garcia J. Effect of diosgenin on cholesterol metabolism. J Atheroscler Res 1962; 2:459-470.
43. Ulloa N, Nervi F. Mechanism and kinetic characteristics of the uncoupling by plant steroids of biliary cholesterol from bile salt output. Biochim Biophys Acta 1985; 837:181-189.
44. Choe MA, An GJ, Lee YK, Im JH, Choi-Kwon S, Heitkemper M. Effect of early low-intensity exercise on rat hind-limb muscles following acute ischemic stroke. Biol Res Nurs 2006; 7:163-174.
45. Majchrzak KM, Pupim LB, Flakoll PJ, Ikizler TA. Resistance exercise augments the acute anabolic effects of intradialytic oral nutritional supplementation. Nephrol Dial Transplant 2008; 23:1362-1369.
46. Uchida K, Takase H, Nomura Y, Takeda K, Takeuchi N, Ishikawa Y. Changes in biliary and fecal bile acids in mice after treatments with diosgenin and beta-sitosterol. J Lipid Res 1984; 25:236-45.
47. Timmis AD. Early diagnosis of acute myocardial infarction. British Medical Journall990 27;301(6758):941-2.
48. Holland RP, Brooks H.TQ-ST segment mapping: critical review and analysis of current concepts. Am J Cardia! 1977; 40 110-129.
49. Nariander NN, Dhawan S. Isoproterenol induced myocardial necrosis and biogenic amine levels in rats. Indian J Exp Bioll979; 17292-294.
50. Kela AK, Reddy LP, Thombre DP. ECG findings in normal rats and after administration of isoproterenol. Indian JPhysiol Phannacoll980; 24:84-90.
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